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The idiotype TCR Vα1-pIRES-TCR Vβ8 eukaryotic vectors were developed successfully, it would be provided the basic data for the study on specific TCR gene modified T cells.

成功构建了独特型TCR Vα1-pIRES-TCR Vβ8双基因表达载体，为利用特异性TCR基因修饰T细胞的研究提供了基础资料。

Two idiotype TCR Vα1-pIRES-TCR Vβ8 recombinant eukaryotic vectors weredeveloped successfully, and the expression of both idiotypic TCR mRNA and proteincould be detected in transferred A549、K562 and Molt4 cells.

2成功构建了两组TCR Vα1-pIRES-TCR Vβ8表达载体，该表达载体转染A549、K562和Molt4细胞后，可表达相应的mRNA和蛋白。

The rearranged idiotypic CDR3 fragments coding TCR Vβ8 of the Jurkat cell strain and TCR Vβ2 of the Molt4 cell strain were amplified using RT-PCR, and then were cloned into pIRES vector respectively.

用RT-PCR方法扩增特异性重排的Jurkat细胞TCR Vβ8 CDR3序列和Molt4细胞TCR Vβ2 CDR3序列，分别克隆到真核表达载体pIRES中。

The results showed that under 200 W power, in the present of various alkalis, e.g. Na2CO3, NaF, KF, NaHCO3, and DMF-H2O (V∶V=5∶3) used as solvent, PVC-TETA-Pd could catalyze sodium tetraphenylborate to react quantificationally with iodobenzene in 20 min without inert gas protection, and all the phenyl g...

实验发现：在微波功率为200W时，在Na2CO3，NaF，KF，NaHCO3等多种碱的存在下，以DMF的水溶液(V∶V=5∶3)为溶剂，在无惰性气体保护下，PVC-TETA-Pd催化四苯硼钠与碘代苯在20min内即可定量反应，与常规加热方式相比，节省了大量时间；同时也证明了四苯硼钠的四个苯基均可参与Suzuki偶联反应；该催化剂回收再利用3次后，仍具有较高的催化活性。

In order to study the mechanism of V-type CMS wheat, a set of near isogenic lines of restoring gene to wheat V-CMS were used as materials, the mtRNAs of male sterile line, maintainer line and F1 hybrid were compared by cDNA-RAPD method.

为了研究V型细胞质雄性不育小麦的不育机理，以V型细胞质雄性不育小麦的一套近等基因系为材料，利用cDNA-RAPD方法对不育系、保持系和杂种F1线粒体RNA进行了比较分析。

LaNi〓 catalyst was metallurgically combined with V、Nb and TiFe by ball milling, and then V-LaNi〓、Nb-LaNi〓 and TiFe-LaNi〓 nano-compound materials formed.

利用机械球磨方法使LaNi〓催化剂有机地与室温难活化储氢金属V、Nb、TiFe合金结合，形成V-LaNi〓、Nb-LaNi〓、TiFe-LaNi〓纳米复合储氢材料。

The analytes were extracted with 0.2% metaphosphoric acid/methanol (6:4, v/v) then cleaned up by three solid-phase extraction cartridges (Oasis HLB, Oasis MAX and Cl8), and had better results from Oasis HLB cartridges. The recoveries for oleandomycin and erythromycin analysis by developed method were

萃取净化之方法，经测试以0.2%偏磷酸与甲醇(6：4， v/v)之混合液，比较三种固相萃取匣（Oasis HLB， Oasis MAX及C18），结果以利用Oasis HLB固相萃取匣萃取及分析，可得到最佳之回收率及净化效果，且具有良好之再现性。

The HPLC condition consisted of C18 column(250 mm×4.6 mm,5 μm),mobile phase of 0.1 mol/L monopotassium phosphate-methanol(70 : 30,v/v) at the flow rate of 1.0 mL/min and at detection wavelength 280 nm.

本法采用C18色谱柱，流动相为0.1mol/L磷酸二氢钾溶液(用磷酸调节pH值至2.5)-甲醇的混合溶液(按v∶v=70∶30配制)，检测波长为280nm，流速为1.0mL/min。

Degree sum conditions of IM-extendable graphs are researched. The main results are as follows:(1) Let G be a graph with 2n vertices. If for each pair of nonadjacent vertices u and v in G,, then G is IM-extendable.(2) Let G be a claw-free graph with 2n vertices. If for each pair of nonadjacent vertices u and v in G,d+d≥2n+3, then G is IM-extendable.

研究导出匹配可扩图的度和条件，主要结果如下：(1)若图G有2n个顶点，且对于G中每一对不相邻的顶点u和v，，则G是导出匹配可扩的；(2)若G是一个有2n个顶点的无爪图，且对于G中每一对不相邻的顶点u和v，d+d≥2n+3，则G是导出匹配可扩的。

Degree sum conditions of IM-extendable graphs are researched. The main results are as follows 1 Let G be a graph with 2n vertices. If for each pair of nonadjacent vertices u and v in G,, then G is IM-extendable. 2 Let G be a claw-free graph with 2n vertices. If for each pair of nonadjacent vertices u and v in G,du+dv≥2n+3, then G is IM-extendable.

研究导出匹配可扩图的度和条件，主要结果如下 1若图G有2n个顶点，且对于G中每一对不相邻的顶点u和v，，则G是导出匹配可扩的 2若G是一个有2n个顶点的无爪图，且对于G中每一对不相邻的顶点u和v，du+dv≥2n+3，则G是导出匹配可扩的。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。