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MicroRNAs primers were arrayed on plates according to the Tm of each primer. PCR were carried out at different annealing temperatures using a gradient real-time PCR instrument. The relative expression level of each microRNAs was calculated using the most stable reference gene as normalizer which was selected by geNorm and Normfinder software.

通过geNorm软件和Normfinder软件分析选择最佳的内参照基因，将每种microRNAs按其引物Tm值排成阵列，用梯度实时PCR仪在不同的退火温度扩增，并计算每种相对于microRNAs内参照基因的表达量。

Using both hands, mould noseclip to the shape of the lower part of the nose, do not pinch the nosepiece using only one hand as this may result in less effective respirator performance.

用两只手，把鼻夹变成鼻子下部的形状；不要只用一只手去捏管口，这样可能会导致呼吸器效力减轻。

You can connect your computers either by using a null modem serial cable or by using Home networking.

您可以用假调制解调器串行电缆或家庭联网来连接计算机。

This past year, spammers were seen using attachments as well as using more advanced identity obfuscation techniques.

过去这一年，垃圾邮件发送者被认为是使用附件，以及使用更先进的身份模糊手法。

This paper chose Carbendazim, Daconil, Imidacloprid and Methomyl as experiment objects, determined their influence to soil respiration of field, shed and school using obturate direct absorb method; discussed not only the variety of soil respiration under different pesticide's sorts, concentration and soil conditions but also the relationship between soil respiration and the variety of bacteria number in soil; confirmed the toxicity grade and dangerous index; provided an scientific basis for environment safety evaluation of pesticide's using.

因此，本论文选取多菌灵、百菌清、吡虫啉、万灵为受试农药，用密闭直接吸收法测定了农药对三种土壤（农田土、大棚土和校园土）呼吸作用的影响；讨论了农药种类、浓度、土壤条件的改变引起的土壤呼吸作用强度的变化及土壤呼吸量与土壤中细菌数量变化的关系；确定了农药的毒性等级和危害系数，为农药使用的环境安全性评价提供科学依据。

In this system, the combined orientation model of DTWADGPS is established using the WADGPS, odograph, speedometer and roscope, etc. And the information of the multi-sensor is processed using the method of data fusion, which is used to resolve the accuracy and blind area problems of train position Combining the technology of GIS and GSM-R and the Expert System, the system of intellectualized train dispatch and management is established based on train positions and velocities acquired accurately.

系统采用WADGPS、里程表、车速仪、陀螺仪等构建分布式逆向WADGPS组合定位模型并对多传感器信息进行融合处理，解决列车定位的精度和盲区问题；在精确获取列车位置和速度的基础上，结合GIS和GSM-R技术和专家系统构建列车智能调度管理系统。

So far as current China is concerned, the leading value outlook sho uld be socialism with Chinese characteristic, and it should be developed in t he whole society by using the value yardstick of integrating the productivity pr ogress and individuals development, by using the value criteria of laying equal emphsis on scientific and humanism spirit.

对于现阶段的中国，主导价值观应是有中国特色的社会主义价值观。在全社会培育这种价值观，要坚持生产力进步与个人全面发展相统一的价值尺度，要坚持科学精神与人文精神并重的价值标准。

Nepetalactone, the primary component of catnip oil, were extracted by the using method of simultaneous distillation-extraction and separated by using silica gel chromatography column. To compare the repellency of nepetalactone and DEET on mosquitoes, topical bioassay method on the skin and a cage olfactometer were employed to assess the effective protection time and the spatial repellency.

采用同时蒸馏萃取及硅胶柱层析法从猫薄荷Nepeta cataria植物中提取、分离制得驱蚊有效成分假荆芥内酯，并采用个体涂肤有效保护时间测定法及风洞空间驱避效果测试法，对假荆芥内酯与避蚊胺的驱蚊效果进行了对比试验。

Hook. F., behavioral tests using four-armed olfactometer and elecroantennograms responses using EAG recording technique in Liriomyza sativae, were all studied.

生物测定结果表明，每棵豆苗施用1～5 μL挥发油，大部分处理的落卵量明显低于对照，其中5 μL剂量效果最好，干扰作用指数和驱避率分别为0.289、55.14﹪。

Following that, we implement the algorithms of FOC, space vector pulse-width modulation, and speed control using the digital signal processor. Regarding to the influence of controller parameters on dynamic response of the motor speed, this thesis proposes the speed control of PMSM using self-tuning fuzzy technique. By the self-tuning technique, the on-line fuzzy logic controller output can give better speed response under the load disturbances or variable speed commands.

本文首先使用数位讯号处理器完成磁场导向控制、空间向量脉波宽度调变及速度控制之策略，针对控制器参数对系统动态响应之影响，提出采用自调式模糊控制器於速度控制回路，藉由自调式机制，线上调整模糊控制器之参数，使PMSM在变动负载及速度命令下有较佳之动态响应，达到提升系统响应及强健性之目的。

The objective is to subjugate and discourage the people, because that allows the elite to continue to rule unopposed.

其目的是压制和打击人民的积极性，因为这可以让实权派继续统治不会沦为反对派。

GOD,this is the second time you vanquished me!

天啊，这是第二次你打败了我！