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Total genomic DNA was extracted from four marine fishes (Verasper variegates, Cleisthenes herzensteini, Turbot Scophthalmus maximus, red seabream Pagrosomus major) using a proteinase K and phenol-chloroform procedure. Long mtDNA fragments were amplified by long and accurate PCR using primers designed based on the mtDNA sequenses of Thunnus thynnus thynnus, flounder Paralichthys olivaceus and other related fishes.

用蛋白酶k酚氯抽提法从圆斑星鲽、高眼蝶、大菱鲆和真鲷4种海水鱼的肌肉组织中提取基因组DNA，根据金枪鱼、牙鲆及相关鱼类的线粒体DNA序列保守片断设计出扩增引物，扩增从16SrRNA到ND4之间约9400bp的mtDNA长片段。

In this paper, about how to realize the wired television bi-directional HFC network technical problems of " the collecting disturbance" and "collecting equalization" were presented because of using the co-axial cable as transmission medium of the branch and going-to-some place by distribution have been solved, and beginning with the noise disturbance analysis, some methods and countermeasures of reforming and settling the problem have been put forward using part of experimental data and theoretic calculation .

本文就如何实现有线电视双向HFC网，解决因以同轴电缆为传输媒质的支线和分配入户网中，存在着"汇集干扰"，"汇集均衡"等技术问题，从噪声干扰分析入手，引用部分实验数据和理论计算，提出了改造解决的一些方法与对策。

A 1172 bp fragment was detected for Wx-7A gene using dominant marker primer from the Wx-A1 normal wheat, a 593 bp and a 574 bp fragment were detected using codominant marker primer from the Wx-A1α normal and Wx-A1 null wheat, respectively.

STS标记结果中，Wx-7A位点的显性标记引物在野生型中扩增出一条1172bp的特异带，即Wx-A1α基因出现；Wx-7A位点的共显性标记引物在野生型中扩增出一条593bp的特异带，在缺失类型中扩增出一条574bp的特异带。

Guglielmo Marconi, recognizing the possibility of using these waves for a wireless communication system, gave a demonstration (1895) of the wireless telegraph, using Hertz's spark coil as a transmitter and Edouard Branly's coherer (a radio detector in which the conductance between two conductors is improved by the passage of a high-frequency current) as the first radio receiver.

Guglielmo Marconi，认可使用这些波浪的可能性为一个无线通信系统，给了一次示范(1895)无线通信机，使用赫兹的火花卷因为发射机和Edouard Branly 的coherer (导率在二个指挥之间被高频率潮流段落改进)的一台无线电探测器如同第一无线电接收机。

A rapid, efficient protocol to detect the toxicity of Cu2+ stress using wheat coleoptile, which is simple, uniformal in structure and sensitive, was established. By using this model, the mechanism of Cu2+ stress leading to the inhibition of cell elongation was also studied.

本文以小麦为材料，研究不同浓度Cu2+对小麦种子萌发和幼苗生长的影响及机理；利用小麦胚芽鞘结构简单、反应敏感的特点，探索建立小麦胚芽鞘快速检测铜离子毒害模式，并利用此模式研究了铜离子胁迫对细胞伸长抑制作用的机理。

It was difficult to estimate the model parameters by using conventional methods due to the model collinearity, so a new compensation method using the finite impulse response model was proposed.

鉴于模型参数之间存在很强的复共线性，提出了基于有限脉冲响应模型飞机磁场补偿方法。

This paper puts forward to a new way of generating colored noise using a novel all-filter phase digital filter. Compared to traditional FIR filter, the colored noise generated by using this filter has fewer spectrum leak, higher stop band attenuation.

本文提出用全相位数字滤波器这种新型滤波器滤波白噪声产生有色噪声的方法，这种方法比用传统FIR滤波器产生的有色噪声频谱泄漏少，阻带衰减功率大等优点。

The second option is to extract the track directly from the aerogel in a "keystone", using an extraction technique that we developed here at Berkeley specifically for Stardust and have been using successfully for several months on the cometary collector.

第二种方案是直接从气凝胶中提取出含有轨迹的楔形块，用的是我们在伯克利特别为 Stardust 开发并在数月前成功用在彗星收集器上的提取技术。

PbWO4 and CeF3 which are newly developed inorganic scinti1latr in our country have been studied. Photo-electricity detector systemes which have high γ sensitivity and relative insensitive to neutron and fast time response were assembled by using CeF3 and photomultiplier tube. The ratio of signal-to-noise for γ ray detection can be up to more than 10 times in close distance n,γ commix pulse radiation field by using this detector system.

对PbWO4和CeF3等近年国内新研制的无机闪烁体进行了系列研究，使用CeF3分别配光电倍增管和光电管，组合出了对γ辐射灵敏度高，对中子相对不灵敏，同时脉冲响应也快的光电探测系统，应用以此为基础的探测技术在近距离n，γ混合脉冲辐射场中，可将脉冲γ辐射探测信噪比提高一个量级以上。

You do this by using a general name, called a noun or common noun , or by using a specific name called a proper noun .

你通过使用一个通用的名称来提起某事，这种通用的名称被叫做"名词"或者"普通名词"，你也许还会使用一个特殊的名字来提起某事，这种特殊的名字叫做"专有名词"。

The X-ray diffract was used to analyze the crystal structure of the composite. Results show that after the talc was treated with blend coupling agent, the crystal structure of talc changed, and effect Intercalation phenomenon.

通过X射线衍射分析可知，用混合偶联剂对滑石粉进行预处理后，滑石粉晶体结构发生改变，产生了插层现象，使PP／滑石粉复合材料为纳米复合材料。

An authentic Maui wedding might require the services of a 'kahu', which is a Hawaiian minister.

毛伊可能需要一个真实婚礼服务'kahu'，这是夏威夷部长。