查询词典 U.C.

Recombinant ptotein made up 39.2%(pET-20b-bglA) and 33.5%(pET-28a-bglA) of the total proteins in the intracellular fraction, the solubility proportion of the enzyme up to 32.8%(pET-20b-bglA) and 40.1%(pET-28a-bglA), the activities of the enzyme were 66.8 (pET-20b-bglA) and 71.2 U/mg (pET-28a-bglA). These results showed that E. coli BL21-CodonPlus (DE3)-RIL with argU tRNA, ileY tRNA and leuW tRNA genes helped to improve expression of the enzyme through enhanced identification of the rare codons AGA/AGG, AUA and CUA. Further optimized the conditions for inducing, the solubility proportion of the enzyme was 70.6% at 16 °C and 1.2 times higher than 37 °C. The solubility proportion of the enzyme increased from 12.3% to 32.8% when IPTG concentrations dropped from 1000 μM to 25 μM. The recombinant enzyme was purified by heat treatment, DEAE Sepharose anion-exchange chromatography and TOYOPEARL HW-55F.

maritima MSB8 的-葡萄糖苷酶基因 bglA克隆至表达载体 pET-20b 和 pET-28a，构建重组质粒 pET-20b-bglA 和 pET-28a-bglA，然后转化不同大肠杆菌 E.coli 宿主，Tm-SIGlA 在 E.coli BL21-CodonPlus(DE3)-RIL 中获得高效表达，重组蛋白的表达量为 33.5%(pET-28a-bglA)和 39.2%(pET-20b-bglA)，可溶性比例为 32.8%(pET-20b-bglA)和 40.1%(pET-28a-bglA)，比酶活达 66.8 (pET-20b-bglA)和 71.2 U/mg (pET-28a-bglA)，这些结果表明，E.coli BL21-CodonPlus(DE3)-RIL 宿主带有的 argU tRNA、ileY tRNA 和 leuW tRNA 基因，分别增强对稀有密码子 AGA/AGG、AUA 和 CUA 的识别，有助于提高该酶在 E.coli 中的表达；进一步优化诱导条件，重组 E.coli BL21-CodonPlus(DE3)-RIL/pET-20b-bglA 在 37 ℃下诱导培养，IPTG 浓度由 1000 μM 降至 25 μM，目的蛋白可溶性表达由 12.3%增至 32.8%，提高 1.7 倍，16 ℃下低温诱导实现目的蛋白 70.6%的可溶性表达，较 37 ℃下诱导培养提高 1.2 倍。

The Meingitis C vaccine is designed to protect against bacterial disease caused by the C strain of the meningococcus pathogen—just 20 percent of all cases in the U.S. and 40 percent in the U.K.

C型脑膜炎疫苗是设计用来预防C类流脑病原体引起的细菌疾病—所有病例中这种病例在美国只占20%，英国只占40%。

You can also use the /U command-line option to unprotect the primary file, and the /C option to clear the keys for that project.

你也可以使用/U命令行选项取消主要文件的保护，/C选项清除该工程的密匙。

Present fluidized bed designs do not consider the effect of biofilm, but the fluidization of bioparticles is different to that of smooth carriers, thus terminal free-fall velocity u in Richardson-Zaki equation and drag coefficient C should be modified.

目前流化床设计一般没有考虑生物膜的影响，但生物颗粒的流化行为有别於光滑载体，因此需要对曳力系数C和膨胀特性方程中的u进行必要的修正。

In 2004 Zhang Zhongfu presented the concept ofadjacent vertex distinguishing total coloring whose content is: Let G be a connectedsimple graph with order at least 2, k be a positive integer and f be a mappling fromV∪E to {1,2,...,k}. Let C={f}∪{f|uv∈E,v∈V}for all u∈V. If (1) for any uv, vw∈E,u≠w, we have f≠f;(2) for any uv∈E, we have f≠f, f≠f, f≠f,then f is called a k-proper total coloring of graph G.

第三章和第四章主要研究了两种联图的邻点可区别全染色，邻点可区别全染色的定义是张忠辅在2004年提出来的，其内容为：设G是阶至少为2的简单连通图，k是正整数，f是V∪E到{1，2，…，k}的映射，对任意u∈V，记C={f}∪{f|uv∈E，v∈V}。

Next,similar to the studies of Friedland-Hersonsky inequalities in norm form and Martininequalities for discrete groups of 〓,we establish 〓 type inequalitiesfor subgroups of unitary transformation group U(1,n,C)in complex hyperbolicspace 〓 and we also give several 〓 type inequalities in the form of fgfg〓for the norm algebra 〓 in C.

其次，我们对复双曲空间〓上的酉变换群U(1，n，C)的子群建立了〓型不等式，推广了Martin的相应结果。最后，我们对复数域C上的范数代数〓建立了形如〓的〓型不等式，推广了Friedland-Hersonsky不等式。

BMSCs were isolated from adult rats by modified bone marrow culture method. BMSCs were inoculated in basic medium containing 10%fetal bovine serum, high glucose DMEM, 100 U/L penicillin, 100 U/L streptomycin and 2 mmol/L glutamine in the control group. BMSCs were inoculated in conditioned medium (basic medium, 10 mmol/L β-glycerophosphoric sodium, 10-7 mol/L dexamethasone, 50 mg/L vitamin C).

通过改良的骨髓培养法分离成年大鼠骨髓间充质干细胞，对照组以基础培养液(10%胎牛血清+高糖DMEM+100U/L青霉素+100 U/L链霉素+2 mmol/L谷氨酰胺)培养，实验组用条件培养液(基础培养液，10 mmol/Lβ-甘油磷酸纳，10-7 mol/L地塞米松，50 mg/L维生素C)进行诱导培养。

In this study, bound residues of [C ring-U-14 C] ZJ0273 in three kinds of soil and dynamics distribution in humus fulvic acid, humin and humic acid were studied by using 14C isotope tracer technique.

利用14C同位素示踪技术研究了［C环－U-14C］丙酯草醚(ZJ0273)在3种不同类型好氧土壤中的结合残留及其在富啡酸、胡敏酸和胡敏素中的动态分布。

Methods:All the 40 Winstar rats were divided into four groups:group A:the rats were fed with normal diet,group B:the rats were fed with only high|cholesterol diet(no vitamin D3),group C:the rats were treated with one intramuscularly injection of vitamin D3(300 000 U/kg) and were denudated of endothelium,followed by high|cholesterol diet,group D:the rats were treated with 5% chitosan added into the diet besides treatment as group C.Ninety days later,the formation of atherosclerosis plaques and the activities of nitric oxide synthase in aortas in the four group rats were investigated.

40只Wistar大鼠均分为：A组；B组：饲以高脂饲料（不含维生素D3）；C组：一次性给予大鼠维生素D3（30万U/kg体重）肌肉注射，以球囊损伤主动脉内皮和饲以含维生素D3（1.25×106U/kg）的高脂饲料；D组在C组的基础上饲料中加入5%壳聚糖。90 d后检测主动脉动脉粥样硬化斑块形成及NOS的活性。

The 4th left rib was cut and a model of left coronary artery occlusion/release was carried outo Rats were divided randomly into 4 groups: control group without LCA occlusion (group A, n=6);I/R group(group B, n=6); ketamine(5mg.kg-1)+I/R group (group C, n=6); ketamine(10mg.kg-1)+I/R group(group D,n=6)o All rats in groups B, C , D were subjected to 30 minutes of LCA occlusion followed by 120minutes of reperfusion. Rats in group C and D were injected with 5mg.kg-1 and 10mg.kg-1 of ketamine before reperfusion, respectively. Significant electrocardiogram and color changes at the area at risk were considered indicative of successful coronary occlusion and reperfusionc Serum was exampled from left jugular vein at 30 minutes and 120 minutes during reperfusion to measure IL-6 and TNF- level by ELISA measurements. After reperfusion of 120 minutes, the heart was removed and the cardiac apex was exampled with snap-frozen in liquid nitrogen and stored at -70 C .

采用左冠状动脉前降支结扎开放建立心肌缺血／再灌注模型，健康SD大鼠24只，随机分为心包打开假手术组(A组，n=6)，缺血／再灌注对照组(B组，n=6)，5 mg·kg~(-1)氯胺酮+缺血／再灌注组C组，n=6，缺血30min后右腹股静脉注入5 mg·kg~(-1氯胺酮，10mg·kg~(-1)KTM+I／R组D组，n=6，缺血30min后右腹股静脉注入10 mg·kg~(-1氯胺酮。10％水合氯醛40 mg·kg~(-1)腹腔注射麻醉，气管切开，连接多功能监护仪记录心电图；小动物呼吸机人工呼吸，呼吸频率60次／分，潮气量2ml／100g，于左胸第四肋间打开胸腔暴露心脏，在左心耳下1mm左冠状动脉处，用丝线，眼科外用不锈钢小圆针穿过心肌浅层，稳定10min后将U型含有铜丝的胶管置于冠状动脉表面一起结扎(A组不结扎，B、C、D组结扎)；结扎开始左心室心尖部即由红色变暗，30 min后呈暗红色，心电图中出现S-T段抬高，说明缺血形成。

I can still vividly recall the impressive scenes you gave. You were among my friends and relatives who accompanied me from my house to the seaport and when the porters were slow to show up, you obligingly carried my suitcase for me.

你对我的种种感人情意，使我记忆犹新，那天你随着我的亲友从家里送我上轮船，搬运工迟迟不来，你毅然替我提着皮箱上船。

St. Nicholas became the patron saint of Russia, where he was famous for his red cape, flowing white beard, and bishop's mitre.

圣尼古拉成了俄罗斯人的守护神，在那里，他以身披红斗篷，留着飘逸的白胡须，戴着主教法冠而闻名。