查询词典 Tilletia

Bunt A seed-borne disease of wheat and other grasses, caused by the basidiomycote Tilletia.

腥黑穗病：由担子菌 Tilletia 引起的小麦或其他草类的种子疾病。

The internal reference was established by the universal primer pair CQUTC6/CQUTC7 for Tilletia genera, which could be used to determinate the reality of PCR system and exclude both false positive and false negative.

以腥黑穗菌属通用引物对CQUTC6/CQUTC7为内置对照，可以确定被检样品是否含PCR抑制物质进而判断检测体系是否正确，同时有效地排除样品检测结果的假阳性和假阴性。

The lab is capable of quarantine hazardous biology such as : Phytophthora sojea , Tilletia controversa , Bursaphelenchus xylophilus , Sorghum halepense , Acanthoscelides obtectus , Bean Pod mottle virus , and practising diagnosis akabane disease , leukaemia bovum,paratuberculosis and other hazardous animal disease;detection of listeria monocytogenes , staphylococcus aureus and salmonella etc in food;examination of transgenic products such as soybean , rapeseed , corn etc and detection of bovine ,sheep and goat -derived for preventing bovine spongiferm encephalopathy .

检测的项目主要有：大豆疫病、小麦矮腥黑穗病菌、松材线虫、假高梁、菜豆象、菜豆英斑驳病毒等植物危险性有害生物，赤羽病、白血病、副结核等动物危险性有害生物，单增李斯特氏菌，金黄色葡萄球菌，沙门氏菌等致病性食品微生物，大豆、油菜籽、玉米等转基因产品，以及预防疯牛病的牛羊源成分检测等。

The plant Quarantine Lab had intercepted more than 200 quarantine pests such as : Tilletia indica , Tilletia controversa , Bursaphelenchus xylophilus , sorghum halepense , Acanthoscelides obtectus , Sternochetes mangiferae , Bactrocera dorsalis , Coptoermes curvignathus for nearly 5000 batches ; The Animal Quanantine Lab had intercepted about 20 cow which had some pathogens evidence of infectious disease such as : bovine infectiouse rhinotracheitis , leukaemia bovum , paratuberculosis and so on ; The food microorganism lab had detected over 300 unqualified products such as Salm-Sury , Listeria monocytogenes , Mycobacterium paratuberculosis etc .

多年来，分中心各实验室切实履行严格把关的职责，植物检疫共截获大豆疫霉病菌、小麦印度腥黑穗病菌、小麦矮腥黑穗病菌、松材线虫、假高梁、菜豆象、芒果果核象甲、桔小实蝇、大家白蚁等国家禁止进境的危险性有害生物220 多种近10000 批次；动物检疫发现感染牛传染性鼻气管炎、牛白血病、副结核等传染病的疫牛20 多头；食品微生物检测共检测沙门、单增、副结核的不合格商品300 多批；分子生物学实验室为国内企业出具非转基因证书和不含有牛羊源成分证书50 余份，使相关的产品能够顺利出口到欧盟、韩国和日本等国。

Result The detection limit of the two systems were 0.1fg, equal to 2.31×10^4 copies, which was 10^2-10^3 fold higher than conventional PCR. By the constructed detection systems, the TCK and Tilletia cariesTul could be distinguished. The teliospore and mycelium of TCK in the infected wheat plant tissue also could be identified accurately and rapidly.

建立的两套定量PCR检测体系的检测下限相当，可达到0.1fg，对应的拷贝数为2.31×10^4个，检测灵敏度比常规PCR高2~3个数量级，均可成功鉴别出TCK与小麦网腥黑穗病菌Tilletia caries (DC Tul， TCT，并可快速准确检测小麦矮腥黑穗菌冬孢子和检测罹病小麦植株体内的侵染菌丝体。

Infection occurs during the flowering stage of the host plant, when its developing ovary comes into contact with infectious sporidia, a stage in the lifecycle of the pathogen Tilletia indica.

寄主植物在开花期受侵染，病原菌Tilletia.indica生活史某一阶段产生的孢子侵入发育的子房。

Examples of smuts are Tilletia and Ustilago.

例如腥黑粉菌属 Tilletia 和黑粉菌属 Ustilago 。

Tilletia controversa Kühn, one of the important international quarantine fungal pathogens, causes wheat dwarf bunt disease which distribute in America, Europ, Northern Africa and West Asia, especially in 7 Northwest states of USA.

小麦矮腥黑穗病(Wheat dwarf bunt disease， DB)是麦类黑穗病中危害最大、极难防治的国内外检疫性病害之一，是由小麦矮腥黑穗病菌(Tilletia controversa Kühn， TCK)引起的。主要分布在美洲、欧洲、北非和西亚，尤其是美国西北部7州。

To detection of Tilletia controversa Kühn sensitively and accurately, real-time PCR systems were developed. The species-specific primer pair CQUTCK4/CQUTCK5 and probe CQUP1 were designed based on a selected specific fragment (1322 bp) specific for TCK, and the SYBR Green I and TaqMan quantitative PCR detection systems were established with optimized reaction conditions.

建立荧光定量PCR体系以准确灵敏的鉴定小麦黑穗菌(Tilletia controversa Kühn， TCK)根据筛选的TCK独有差异基因片段(1322bp)设计特异性引物对CQUTCK4/CQUTCK5和TaqMan探针CQUP1，建立SYBR GreenⅠ荧光染料法和Taq Man水解探针法定量PCR检测体系，并对体系进行优化。

Fungal isolates which belong to 28 or more genera, were identified by standard blotter method. The most common fungi isolated were Aspergillus (11.66% of all fungal isolates), Tilletia horrida (10.54%), Fusarium (9.36%), Rhizoctonia solani (8.07%), Penicillium (7.54%), Alternaria (4.95%), Rhizopus (4.59%), Ustilagiiwidea virens (4.18%), Sclerotium (4.12%), Mucor (3.36%) species.

这些真菌菌株分属于至少27个属，其中以Aspergillus（占总菌株的11.66%）、Tilletia horrida (10.54%)、Fusarium (9.36%)、Rhizoctonia solani (8.07%)、Penicillium (7.54%)、Alternaria (4.95%)、Rhizopus (4.59%)、Ustilaginoidea virens (4.18%)、Sclerotium (4.12%)、Mucor (3.36%)这10个属种为优势菌群，合计占总菌株数的68.37%。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。