查询词典 Theileria

Two phylogenetic trees was inferred based on 18srRNA sequence of Hebei, Liaoning province, Lintan, Ningxian in Gansu and the other species of Babesia and Theileria available in GenBank.

结果发现自河北、辽宁、临潭、宁县四个地区分离株建立的进化树上，临潭与宁县的虫株亲缘关系较近，同源性是91.4％；而辽宁与河北的同源性是95.8％。

To evaluate the specificity of the PCR, genomic DNA of Theileria annulata,Babesia bovis,Toxoplasma gondii,Leishmania donovani and standard strain of N. caninum were used as a template in the PCR. For determining the detection limit of amplification procedure, PCR was run on a dilution series of genomic DNA from N. caninum（1.562 5-200 ng/ml）. Brain tissue samples of 32 aborted fetuses were detected by PCR-based assay, and 23 blood samples from mothers were tested by ELISA. Results The amplified DNA fragment （350 bp）had a high identity of 98% with the Nc-5 gene sequence of N.

以环形泰勒虫、牛巴贝斯虫、刚地弓形虫、杜氏利什曼原虫以及犬新孢子虫标准株DNA为模板进行扩增以验证PCR的特异性，采用紫外分光光度计测定犬新孢子虫标准株DNA浓度和纯度，取高纯度的DNA样品用灭菌水稀释，分别取不同量的DNA进行PCR扩增，确定PCR方法的敏感性；利用该方法对32份奶牛流产的胎牛脑组织样品进行检测，同时，对其中23份流产的母牛血样进行ELISA血清学检测，以评价犬新孢子虫PCR方法的检测效果。

According to the Tams1 gene of bovine Theileria annulata published on GenBank, two pairs of primers were successfully designed to develop nested PCR assay for detecting the disease caused by bovine Theileria annulata.

根据GenBank上发表的环形泰勒虫Tams1基因序列设计2对巢式引物，成功建立了环形泰勒虫病的巢式PCR快速检测方法。

In order to analyze gene sequence of the bovine Theileria annulata in Xinjiang, a pair of primers was designed, Tamsl gene fragment was amplified by PCR, sequence of positive clones was showed that the gene fragment has a total length of 846 bp, which encoded 281 amine acids.

采用PCR技术，从新疆焦虫病疫区感染牛的全血中扩增到Tamsl基因，该基因长度为846 bp，编码281个氨基酸。

The subsequent larval and nymphal progeny were applied onto the calves infected with Theileria annulata in order to investigate whether Hy. a. anatolicum were infected by both Theileria annulata

结果显示：巴贝斯虫未定种首先在蟀的肠道内发育，经一系列未知的变化后，在蟀饱血脱落5天内形 2 摘要成运动体，随后，几乎同时可在肠道、血淋巴、唾液腺及卵巢内发现大裂殖体

Theileria annulate; Tams1 Gene; Optimization of Expression; Immunological Reactivity

国家自然科学；牛环形泰勒虫； Tams1基因；表达条件优化；免疫反应

This article reviewed the development and application of the method by taking an example of developing of an LAMP method for detection of Theileria uilenbergi.

作者结合研制尤氏泰勒虫核酸检测实践对该方法的原理，用途和应用中的优缺点进行了综述。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。