查询词典 The Thaw

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

The effects of concentration, temperatrue, rotation rate, pH and salt on the paste property of the colocasia esculenta schott starch were studied. The clarity freeze-thaw, retrogradation and anti-mycotic characteristics were determinated.

研究了糊浓度、温度、回转速度、pH和盐等因素对香芋淀粉糊粘度的影响，测定了香芋淀粉糊的透光率、冻融性、凝沉性和抗霉菌能力等性质。

The diluent contain magnesium chloride, potassium citrate, sodium glutamate, dipotassium hydrogen phosphate, monopotassium phosphate and fructose, semen vitality is hightest after frozen.The average value is 0.52,highter than other 5 diluent significantly (P﹤0.01).So chose this diluent as semen cryopreservation diluent on chicken. Definite cryoprotectant, equilibrium time and thaw temperature:On the base of test one,research freeze sheltering effect about glycerin, DMSO ,DMA and DMF, each with 4 consistency.The result showed that 10%DMSO sheltering effect is the best, the next is 4%DMA,semen vitality are 0.58 and 0.55 after frozen.

冷冻保护剂、平衡时间和解冻温度的确定在试验一的基础上，研究甘油、二甲基亚砜、二甲基乙酰胺、二甲基甲酰胺4种冷冻保护剂在4种浓度下对鸡精子的冷冻保护作用，结果表明，10%DMSO保护效果最好，其次为4%浓度的DMA，精液冻后活力分别为0.58和0.55，两者之间差异不显著(P﹥0.05)。

Compared with the conventionalemulsion polymerization system in the presence of sodium dodecyl benzene sulfonate, the latices prepared by emulsifier-free emulsion polymerization have much more narrow distribution of latex particle sizes (0.2um in diameter), better resistance to electrolytes, pH, freeze-thaw, and better mechanical stability. Not only the tensile strength of the copolymers prepared by this method is obviously increased, but also the water resistance and adhesion capacity are significantly enhanced. On the other hand, the TG results indicate the copolymer has lower thermal stability than conventional copolymer.

实验结果表明，与传统乳液聚合体系相比，所获得的无皂乳液的乳胶粒子大小均一，粒径在0.20um左右；乳液具有优异的稳定性，不受酸碱度、电解质和低温冷冻的影响；附着力和粘接强度明显提高；成膜后显示较高的耐水性；但TG表明乳胶膜热稳定性有所下降。

The fabrics and crystals of one-year ice in the Bohai Sea show that the noncontiguous ice growth rate makes an ice sheet have level layers with different amount of air bubbles in lower part of the ice sheet; typical grain ice and columnar ice occur in the gray ice which grows in stable water; thaw-refrozen ice and rafted ice have their crystal characteristics.

作者中文名：李志军；康建成；蒲毅彬摘要：渤海一年冰的组构和晶体结构表现出的冰的非连续生长速率使得冰层底部具有气泡含量不同的许多分层；静水生长的灰冰具有典型的粒状冰和柱状冰；融化-重冻结冰和重叠冰具有特定的晶体结构。

At present there are mostly Hydrostatic Pressure Theory and Penetrative Pressure Theory with regard to concrete frostbiting principle after setting. But they are not the last word, because the pressure can not be measured directly or calculated exactly. The two theories can triumphantly explain much phenomenon about concrete in the freeze-thaw process.

混凝土硬化后的冻害机理目前主要有静水压学说和渗透压学说，由于不能直接测定或进行准确计算，所以还没有定论，但它们可以成功地解释混凝土冻融过程中的很多现象。

Following is the factors ideating with the surface of old concrete in different way ( including brushing surface , chiseling surface , cleaving surface ), adherence agent ( including cement slurry , cement sand slurry , cement slurry with the u expansion agent ), the freeze - thaw circulation times and air - entraining .

主要考察了老混凝土表面不同处理方式(刷毛面，凿毛面，劈裂面)、界面粘结剂类型(水泥净浆，水泥砂浆， u型膨胀剂水泥浆)、冻融循环次数等对新老混凝土粘结面劈拉强度的影响，以及冻融作用下新混凝土加气对粘结面劈拉强度的影响。

The relation of the water stability of asphalt mixture with the interaction of granite and asphalt was verified by freeze-thaw split test. It is found that there is a good corresponding relationship between the rheological property of granite asphalt mastic and the water stability of the granite asphalt mixture.

并通过冻融劈裂试验检验沥青混合料水稳定性与沥青和石粉交互作用能力之间的关系，发现花岗岩沥青胶浆的流变学性质与花岗岩沥青混合料水稳定性之间存在良好的对应关系。

Sui November 2003 "Shanghai amendment to the Town Planning Ordinance," the beginning of the control center and the Shanghai City Real Estate Development "double double-cut increase" policy measures adopted, Shanghai (Pudong-region) in the time before some of over 200 residential projects were suspended due to planning constraints to development issues thaw, the overall volume of these projects are expected in more than 10 million square meters, but mostly concentrated in Link, Link particularly within the vicinity, consider those items listed preparation period (covering 844,000) is estimated at two years, so the project is expected by the end of 2005 and in early 2006 will begin to enter a large number of Shanghai market, when Shanghai residential market downtown areas, the central area Yishoufang supply will show a big increase.

随2003年11月《上海市城市规划条例修正案》和上海开始控制中心城区房地产开发的&双增双减&政策措施的出台，上海市在此时间段之前约200多个住宅项目，由于规划限制被暂缓开发的问题得以解冻，预计这些项目的总体量在1000万平方米以上，而且大多集中在环线以内尤其是内环线附近，考虑这些项目的上市准备期估计在2年左右，故上述项目预计在2005年底及2006年初将开始大批进入上海市场，届时，上海住宅市场的中心区、次中心区一手房供应量会有较大幅度提升。

While, in order to analyze and assess the change of frozen soil environment, the classification of the thermal thaw-erosion sensibility, the thermal stability and the surface landscape stability of frozen soil is also proposed, which is coordinated with the mean annual ground temperature and freezing-thawing hazards analysis.

为便于评价和分析冻土环境的变化，本文还提出了冻土热融蚀敏感性、热稳定性和地表景观稳定性的划分方法，并使之与冻土年平均地温及冻融灾害相适应。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。