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The results demonstrated that the measured molecular weight of phycocyanin was 37.715Kd, and consisted of two peptides which were similar with their molecular weight and amino acid sequences.4 The comformation of phycocyaninPhycocyanin high structure was investigated, as the result of circular dichroism spectrum and Fourier transformation infared spectrum shown, the secondary structure of NSPC was mainlyα-helix structure and the content ofβ-shee or coil was very small.

根据氨基酸自动分析仪测定的氨基酸组成和精确分子质量计算出NSPC分子总共有331个氨基酸残基，分别是由40个Asp、22个Thr、18个Ser、22个Glu、1个Gly、31个Ala、27个Cys、17个Val、13个Ile、22个Leu、5个Tyr、7个Phe、7个Lys、24个His、51个Arg、14个Pro、1个Met和9个Trp组成。胰蛋白水解酶分别酶解NSPC-1和NSPC-2后，分别采用MALDI-TOF-MS分析，其肽指纹谱图显示胰蛋白酶可将NSPC-1和NSPC-2水解成大小不等的多条肽段，质量数在800以上的NSPC-2有11段，即水解的肽质量分别为899.54、1454.77、1544.9、1683.87、1803.93、1811.91、1987.92、2003.90、2536.26、2915.42和3492.72。

Several coterminous bands could be observed by PAGE of natural Eisenin Ⅰ and several coterminous peaks of Eisenin Ⅰ were also detected with MALDI-TOF-MS when the molecular weight of three main peaks are 24, 645, 25, 052 and 25, 281, separately. The amino acid composition of Eisenin Ⅰ(pI. 8) is specilized with high content of acidic amino acids and low content of alkaline amino acids; At the same time, the content of Ser and Thr are quite high and that of Lys, Met, Phe and Trp are quite low. Eisenin Ⅰ is highly homologous with serine proteases. Then, by fibrin plate assay, Eisenin Ⅰ was identified to be a plasmin and also a plasminogen activator, and the fibrinolytic activity was inhibited by PMSF (an inhibitor of serine proteases).

赤子素Ⅰ氨基酸组成特点为高的酸性氨基酸和低的碱性氨基酸含量，且Ser、Thr含量高而Lys、Met、Phe和Trp含量很低；等电聚焦电泳测得其等电点pI.8.N端序列比较结果显示赤子素Ⅰ与丝氨酸蛋白酶类高度同源，纤维蛋白平板法测得赤子素Ⅰ同时具有纤溶酶和纤溶酶激酶活性；通过PMSF对赤子素Ⅰ纤溶酶活性的抑制实验证明，赤子素Ⅰ属于丝氨酸蛋白酶类。

Methods Proteins and peptides in CSF were separated by two-dimensional gel electrophoresis（2DE） and ultra-performance liquid chromatographer and identified by matrix assisted laser desorption ionization time of flight mass spectrometry（MALDI-TOF-MS） and electrospray ionization to study differential proteomic expression in the cerebrospinal fluid between patients with Demy- elinative disease and with other neurological diseases.

方法以中枢神经系统脱髓鞘疾病患者的脑脊液为研究对象，分别采用固相pH梯度双向凝胶电泳分离总蛋白质，凝胶经银染显色后，用图像分析软件进行比较分析、识别差异表达的蛋白质，离线应用基质辅助激光解析电离飞行时间质谱得到相应的肽指纹图谱；同时用超高压液相色谱分离酶解肽段，在线采用电喷雾四极杆飞行时间质谱鉴定肽段氨基酸序列；搜索数据库鉴定部分蛋白质。

Results Of total 11 patients, 10 cases were mirror-image dextrocardia, 1 case was dextroversion. Among 10 patients were diagnosed as mirror-image dextrocardia, 5 patients were found to have concomitant cardiovascular malformations, the major anomalies were VSD,TOF, DORV. 1 case of dextroversion with complex cardiac anomalies: CECD+SA+DORV+PS. Conclusion Congenital dextrocardia may present with complex anomalies.

结果：11例先天性右位心患者中，镜像右位心10例，右旋心1例。10例镜像右位心中5例合并心内畸形，主要畸形为：室间隔缺损2例，法洛四联症2例，右室双出口1例；超声漏诊2例合并双上腔静脉。1例右旋心合并复杂畸形：完全型心内膜垫缺损、单心房、右室双出口、肺动脉瓣重度狭窄。

They were named respectively as muramidase-released protein; succinate dehydrogenase/fumarate reductase flavoprotein subunit (SDHFp/FRDFP, extracellular); bacterial trigger factor; elongation factor G (EF-G, extracellular); PTS system sorbose subfamily I (PTS/MAN-ⅡAB, cytoplasmic); pyruvate kinase; phosphofructokinase, PK.

经MALDI-TOF-MS鉴定，这8个蛋白分别为溶菌酶释放蛋白、琥珀脱氢酶／延胡索酸还原酶黄素蛋白亚单位(SDHFp/FRDFp)、触发因子、延长因子G、细菌糖磷酸转移酶系统甘露糖特异ⅡAB亚单位、丙酮酸激酶、6-磷酸果糖激酶(6-PFK)、色氨酸－tRNA合成酶。

Centrifuge blood at 4℃and take supernatant,which was preserved in frigidaire at-70℃.(2)Serum protein mass peak were detected by SELDI-TOF-MS and weak canonic chips.

研究方法1。检测宫颈癌早期和中晚期差异蛋白质的表达(1)样品收集：宫颈癌患者共44例，临床分期为早期26例，中晚期18例。

MR angiograms of 204 cases were analyzed retrospectively to sum up the variation ratios of cerebral arteries, their differences in gender and sides, in which the artery not displayed was regarded as absence and the artery 1/2 diameter less than the symmetrical one was regard as hypogenesis.

三、回顾分析204例正常3D-TOF技术MRA三维图象，以MRA未显示为缺如，以成对动脉中一侧直径小于对侧1/2为发育不良，分别统计各动脉的各种变异率，并统计分析其性别及左右侧差异性。

The photoelectron gun was not separated from the ionizer of the TOFMS completely, thus the energy of the photoelectron beam could not be adjusted independently.

但他们所用方法都有一个共同的缺陷，即没有将光电子枪与TOF质谱的离化器完全分离，光电子束能量不能独立调节。

In this paper, using REMPI-TOF technique, multiphoton dissociation/ionization of sulfur and carbon contained polyatomic molecule has been carried out. The mechanism ofmultiphoton dissociation/ionization was discussed, and some valuable results are obtained. The paper has six chapters.

本论文用共振增强多光子电离-时间飞行质谱等实验技术研究了二硫化碳、甲醇和乙醛分子的多光子解离或电离的动力学过程，对多光子解离或电离产物的产生机理进行了分析和讨论，得到了一些有意义的实验结果。

The TOF MS studies of the transition metal / nonmetal cluster ions and Potassium Cyanide clus...

通过对不同条件下形成的团簇离子组成的分析，提出团簇离子形成的可能机理。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。