查询词典 TOF

With the aid of PDQuest 2-DE software, 11 significant protein dots were found out and then 9 of them are pick out to go through peptide mass fingerprinting based on MALDI TOF MS and NCBI database searching . In all, 5 proteins were identified: Ribulose bisphosphate carboxylase , Nucleic acid-binding protein , Ribosomal protection tetracycline resistance protein , hypothetical protein FG04726.1 [Gibberella zeae PH-1], nitric oxide synthase .

应用PMF技术对这11个具有显著表达差异蛋白点中的9个进行鉴定，得到了5个质谱阳性鉴定结果，其中2个蛋白分别为玉米种属的1，5-二磷酸核酮糖羧化酶／加氧酶和叶绿体的核酸结合蛋白；其它的3个蛋白在玉米蛋白质数据库中未见报道，但与其它物种基因编码的蛋白质具有高度的序列同源性。

The good catalytic performances were obtained on those catalysts supported by SiO_2. With the increasing calcination temperature from 300℃to 700℃, Co_3O_4 particles augmented and sintered on the samples surface which contained 10 wt.% cobalt, the crystallite size of Co_3O_4 was aggrandized from 7.2 nm to 20.3 nm by inchmeal. On the other hand, the activation energy for the combustion reaction was increased linearly with the augment of average crystallite size of Co_3O_4, the TOF value on prepared catalyst was decreased with it. Thus, the catalytic combustion was a typical structure-sensitive reaction.

在钴含量为10 wt%的样品上，随着焙烧温度由300℃提高到700℃，钴物种出现迁移团聚甚至烧结，在XRD中测定的Co_3O_4晶粒尺寸由7.2 nm逐步增加至20.3 nm；另一方面，催化剂的燃烧活化能随样品上Co_3O_4晶粒的平均尺寸增加而呈现线形增加趋势，而单位时间内单位活性物种上的甲烷转换频数随着粒径增加而逐渐降低，这一现象证实了该反应属于典型的结构敏感反应。

Tissues of the infundibular septum from 22 patients with TOF were studied and 8 patients without cardiovascular disease and collagen system disease were used as control.The collagen concentration were determined by biochemical methods,the heart function were determined by pressure curve in right ventricle.

用生化方法定量分析22例法洛四联症患者和8例非心血管及胶原系统疾病尸检者的右室心肌间质中的胶原含量和Ⅰ/Ⅲ型胶原比值，并用右室穿刺法测定反映法洛四联症患者右室收缩及舒张功能的dp/dtmax及-dp/dtmax。

The objectives of the study were to investigate the characteristics and influencing factors of myocardial interstitial collagen of right ventricle in tetralogy of Fallot.Tissues of the infundibular septum from 22 patients with TOF were studied and 8 patients without cardiovascular disease and collagen system disease were used as control.Collagen were determined by biochemical methods.

为了解法洛四联症肥大右室心肌间质Ⅰ、Ⅲ型胶原改建的特点及其影响因素，用生化等方法定量分析了22例法洛四联症病人和8例非心血管及胶原系统疾病尸检者的右室心肌间质中的胶原含量和Ⅰ、Ⅲ型胶原比值，以及法洛四联症病人的血氧饱和度等影响因素。

Methods Samples of the infundibular septum from 22 patients with TOF and 8 patients without cardiovascular disease and collagen system disease as control were studied. Collagen was determined by biochemical methods, Pro α mRNA, Pro α mRNA levels were determined by dot blot hybridization.

用生化方法定量分析了22例法乐四联症患者和8例非心血管及胶原系统疾病尸检者的右室心肌间质中的胶原含量和Ⅰ、Ⅲ型胶原比值，以及用斑点杂交法测定法乐四联症患者右室心肌间质Ⅰ、Ⅲ型前胶原基因mRNA的表达。

Immunoprecipitation and matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) were used to isolate and identify proteins which can interact with interceptive AIF respectively.

提取转染截断型AIF基因的细胞的蛋白质，用免疫沉淀方法分离与截断型可能存在相互作用的蛋白质，用基质辅助激光解析电离飞行时间质谱对分离得到的蛋白质进行鉴定，其中一蛋白质条带经鉴定为β-actin。

Based on these consideration, we plan to make a pilot study on the composition of G. Intermedius venom by using two-dimensional gel electrophoresis (2-DE) combined with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS).

然而，至今仍缺乏对其蛇毒组分的整体研究，这不但妨碍其蛇伤的有效治疗，也是限制其开发利用的一个瓶颈。

The basic principles of organic petrographical, organic geochemistry, sedimentology, sequence stratigraphy and the technology of micro-area and spectra analysis have been applied in the paper to study the Jurassic hydrocarbon source rock of the northern edge of Chaidamu basin. The paper reveals the features of the coal petrological, chemical composition and hydrocarbon generating mechanism of the coal bearing source rock in the studied area. It also gives the hydrocarbon generation models of different kerogen types of the area. The technology of 〓CNMR and TOFSIMS are used together to evaluate kerogen's capacity of hydrocarbon generation.

论文运用有机岩石学、有机地球化学、沉积学以及层序地层学的基本原理，综合采用多种微区及谱学分析测试技术对柴达木盆地北缘侏罗系煤系烃源岩进行了系统而深入的研究，揭示了研究区煤系烃源岩的有机组分、组成、地球化学属性以及生烃性质，建立了研究区不同类型干酪根的成烃演化模式并把〓CNMR与TOF-SIMS技术结合起来，应用于干酪根的生烃性质评价。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

Fifty-two protein peaks in the sera were significantly different between 57 patients with tuberculous pleuritis and 70 controls(P＜0.01).The protein peak at m/z 5643 was highly expressed in 47.4%(27/57) sera from the patients with tuberculous pleuritis,and lowly expressed in 4 sera from the patients with non-TB pleuritis.

因此，选择5643 m/z蛋白作为潜在的诊断标志物，经RP-HPLC分离、纯化，并利用MALDI-TOF-MS及LC-MS/MS进行鉴定，5643 m/z目标蛋白质是粘蛋白，又称为α-1-酸性糖蛋白。57例结核性胸膜炎患者血清和70例对照血清蛋白指纹图谱比较，有52个差异蛋白峰(p＜0.01)。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。