查询词典 T.B.

It lied in all kinds of soil and air. B.t had strong insectcidal ability ,and it could kill a lot of creatures of Arthropoda, Protozoa, Nemathelminthes and Platyhelminthes.B.t was the most comprehensive microorganism being used in biotic pesticide.

该病原广泛存在于各种土壤及空气中，具有很强的杀虫活性，至少能杀死节肢动物门九个目及原生动物门、线形动物门和扁形动物门中的多种动物，而对人、畜、哺乳动物及天敌等无害，因此，B.t是生防工业中应用最广泛的微生物。

We present k -Degree T-Bezier basis function and reparametrize it on the interval [0,1],representation of ellipse and cardioid and G~1 condition of T-Bezier curves based on the analysisof T-Bezier basis function and terminal properties. The results have definite geometric meanings that are useful for surface modeling.(3) We presents G~1 of C-B-spline curves and C-Bezier curves.

3给出了C-曲线间的G~1拼接条件，利用C-Bézier曲线能够精确表示半圆弧和半椭圆弧的性质，在实际应用过程中需要精确表示半圆弧和半椭圆弧的地方利用C-Bézier曲线来代替C-B样条曲线，从而较好的解决了在C-B样条造型系统中无法精确表示圆弧的问题。

Let G be semisimple matrix group with Lie algebra g, T a fixed maximal abelian subalgebra of g, T the orthogonal complement of T with respect to the Killing form of g, and a, b 6 T a fixed regular elements.

设G为半单矩阵群，g为其Lie代数，T(来源：ABC论文91网www.abclunwen.com)为g的极大交换子代数，T~⊥为T关于g的Killing型的正交补，a，b∈T为固定正则元。

Methods:The effect of chrysin on HIV1 infection and replication in vitro were detected by cell fusion of MT2 cells and HIV1ⅢB/H9 cells labeled calceinAM and the inhibition study of syncytia formation and p24 production monitored in culture supernatant of MT2 cells infected by HIV1ⅢB, respectively. Flurescence conjugated monoclonal antibodies and flow cytometry were used to detect the expression of CD69 by activated CD4+T in response to PHA.

以CR对CalceinAM标记的慢性感染HIV1ⅢB的H9细胞（H9/HIV1ⅢB）和MT2细胞的融合、对HIV1诱导的MT2细胞形成合胞体及对感染HIV1的MT2细胞产生p24抗原的影响，探讨其抗HIV1活性；以PHA刺激人外周血单个核细胞，利用流式细胞术，检测CD4+T细胞CD69表达的百分率，分析CR对免疫活化的影响。

Results:Biochemical indicators: the concentrations of K+, Na+ and Cl－ in the serum after the infusion are different with those before the infusion; other indicators don't have the significant difference. The mean infusion time of group A is longer than that of group B (p=0.006), while the dosis letalis of KCl doesn't have significant difference between groups (p=0.062); the concentrations of K+ in blood, Na+, Mg2+ and Cl－ in serum have the significant difference between groups; the urine volume and the concentrations of all the urine electrolyte indicators don't have the statistical significant difference between groups.

结果：生化指标：输入钾前后，家兔血清钾离子浓度升高，钠、钙及氯离子浓度降低，具有统计学显著性差异，p=0.000；全血钾及血清其他离子浓度变化，以p=0.05为标准，不具有统计学显著性差异。A、B两组间比较结果：A组输液致死时间长于B组(p=0.006)，致死输钾量无统计学显著性差异(p=0.062)；全血钾、血清钠、镁及氯离子浓度变化值具有显著差异，血清钾、钙、碳酸氢根离子浓度变化值无明显的差异；尿量和尿液各电解质浓度指标无统计学显著性差异。

According to one conserved sequences of phage GIL01 from Bacillus thuringiensis,a pair of primers was designed.With overnight cultivations of 35 B.t standard strains and one fermentative strain MZ1 as templates,PCR results showed that 16 strains,which may be lysogenic strains,produced the product with the size about 750 bp as anticipated.

根据苏云金杆菌(Bacillus thuringiensis， B.t)溶原性噬菌体GIL01(GenBank Accession Number： AJ536703)的同源序列设计一对引物，用35株B.t标准菌株和生产菌株MZ1(血清型H3a3b)的过夜培养物作模板，结果有16株菌株包括MZ1能扩增出大小约为750 bp的预期产物，推测这16株菌为溶原菌。

There are abnormal phenotypes of AChR-specific-responding T、B cell subpopulation in the myasthenic thymus, especially the activated Th cells. In addition,CD5－ B cells might be related to the pathogenesis of the MG.

MG患者胸腺存在着异常的AChR特异应答性T、B淋巴细胞亚群表型，尤其以活化的Th细胞为著；CD5-B细胞的产生可能与MG密切相关。

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我不搞清任何人所能在其他任何人但是你中注意到的我将发现我的nitch在你的小汽车中我不和我的mp3 DVD皱纹-挤满的吉他有关搞清任何人所能在其他任何人但是你中注意到的 Du dudu dudu dududu Dudu dudu dudu duduDu dudu dudu dududu du 是沿着沿着顺而下顺左边权利而下仅仅因为我们使用骗人的东西，左边正确B一出发不意味着我们是不我不在中搞清任何人所能注意到的刺痛其他任何人但是你你是总是设法使它保持真正我是爱你怎样感到我不搞清任何人所能在其他任何人但是你中注意到的我们在两方面同样地富人暴怒的你更多想要的有光泽愉快合身扇，我想要更多舞台我不搞清任何人所能在其他任何人但是你中注意到的&堂吉诃德&是一钢是驱动男人我的名字是亚当我你的我不看见的最大扇子是在任何人所能看见其他任何人但是你的中沿着你的脸扭歪有一点和跳你摇动的舞粪块从出来你的裤子的底部我不搞清任何人所能在其他任何人但是你中注意到的 Du dudu dudu dududu Dudu dudu dudu duduDu dudu dudu dududu du但是你

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

To test this concept in transplant recipients, we evaluated complement, complement regulator, and T cell/proinflammatory marker gene expression by quantitative real-time polymerase chain reaction in 71 archived heart transplant biopsies and correlated the results with the histologic grade of rejection. Significantly more transcripts encoding alternative pathway components factor B, C3 and properdin, and C3a receptor and C5a receptor were detected in grade 3 versus grade 0 or 1 biopsies. The grade 3 rejections also contained significantly higher amounts of CD3, interferon gamma, perforin, and granzyme B genes.

为了明确移植受者中该理论的正确性，我们采用RT-PCR的方法检测了71例心脏移植物活检标本中补体、补体调节物以及T细胞/促炎症反应标记物基因表达及其与排斥反应的组织学分级的相关程度。3级的排斥反应相比于0或者1级的排斥反应其活检标本中旁路途径成分因子B、C3以及备解素和C3a与C5a的受体表达均明显升高。3级排斥分那英同样存在更多的CD3、IFN-gamma、穿孔素和颗粒酶B基因的表达。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。