查询词典 Six Weeks
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The unoperated sides of the treated animals also served as controls. Six normal rats were treated as normal control group. Three different siRNA plasmid solution containing RC2-Ⅰ, MAFbx-Ⅱ, CON (50μl , 0.8μg/μl)was injected and transfected by electroporation as methods mentioned above, respectively. The changes of RC2 and MAFbx mRNA levels and RC2 protein levels after 3 days were determined by real-time quantitative PCR and Western blot, respectively. On postoperative 2, 3 and 4 weeks, the rate of wet muscle weight preservation, mean diameter of muscle fiber and mean cross-section area of muscle fiber and muscle protein content were checked and then compared between group CON and group RC2 or group MAFbx, respectively. The differences between groups were analyzed by one-way ANOVA. Ultrastructural changes of muscle fiber were observed at 2, 3, 4 weeks postoperation.Results GFP plasmid was efficiently deliverd into muscle by electroporation and robust GFP expression in muscle could be observed more than three weeks. Histology shows that injected plasmid DNA diffuses extensively in muscle tissue.
1、健康雌性SD大鼠18只,随机分为电穿孔组和非电穿孔组,每组9只,制作右下肢趾长伸肌失神经支配模型;EP组为将质粒pEGFP-N1溶液50μl(0.8μg/μl)注射入右趾长伸肌后,立即于两侧腱腹交接处给予电穿孔,电穿孔参数为:电场强度为200V/Cm,脉冲100μs,频率1Hz,施加10次脉冲;NEP组仅质粒pEGFP-N1溶液注射;转染后1、2、3周,荧光显微镜下观察趾长伸肌中GFP的表达情况,转染后1周行Western印迹检测趾长伸肌中GFP蛋白的表达情况,检测和优化体内转染效率。2、健康雌性SD大鼠78只,随机分为失神经对照组、RC2基因治疗组(RC2组),MAFbx基因治疗组,每组24只,制作右下肢趾长伸肌失神经支配模型,余6只为正常组;分别将含CON、RC2、MAFbx基因的siRNA重组质粒注射入趾长伸肌,之后给予电穿孔,方法同上;治疗后3天实时定量PCR和Western印迹检测各组中RC2或MAFbx基因的mRNA和蛋白的表达变化,治疗后2、3、4周检测各组肌湿重维持率、肌细胞直径和肌细胞截面积,肌细胞超微结构变化以及肌纤维中蛋白含量变化。
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Methods We randomly assigned 410 patients with locally advanced or metastatic cholangiocarcinoma, gallbladder cancer, or ampullary cancer to receive either cisplatin (25 mg per square meter of body-surface area) followed by gemcitabine (1000 mg per square meter on days 1 and 8, every 3 weeks for eight cycles) or gemcitabine alone (1000 mg per square meter on days 1, 8, and 15, every 4 weeks for six cycles) for up to 24 weeks.
我们将有局部晚期或转移性胆管癌、胆囊癌或壶腹癌的410例病人随机分为两组:一组先接受顺铂(25 mg/m2体表面积)治疗后再接受吉西他滨(1000 mg/m2,第1天和第8天,每3周为1个周期,共8个周期)治疗,另一组接受单纯吉西他滨(1000 mg/m2,第1、8和第15天,每4周为1个周期,共6个周期)治疗,最多治疗24周。
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Sixty six male Sprague-Dawley rats were randomly divided into control group,and 5 model hepatic fibrosis groups perfused alcoholic,maize oil and pyrazole for 2 weeks,4 weeks,8 weeks, 12 weeks and 16 weeks (11 rats in each group) respect...
结果 与对照组比较,酒精灌胃 4周、8周、12周、16周组大鼠OPN、α SMA表达显著增强并且肝内胶原纤维含量显著增加。正常肝组织中几乎不表达OPN ,在酒精性肝纤维化大鼠模型的建立过程中,从第四周开始中央静脉。。。
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If you have given birth less than six weeks ago, the abdominal muscles will be weakened after childbirth and the risk of haemorrhaging increased.
如果您有鉴于出生不到6个星期前,腹部肌肉就会削弱后,分娩和出血的风险增加。
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Six weeks after operation, the rats were killed, with serum and liver taken, and serumglycerol,cholesterol,LDL,HDL and liver homogenate hGT,hGC, measured.
6周后处死动物,测定血清总胆固醇、血清甘油三酯、血低密度脂蛋白、肝组织中甘油三酯和胆固醇含量,取适量肝组织作病理学观察。
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This weekend it will also be six weeks since Owen broke his leg. It happened on 16 September against Arminia Bielefeld. Now the time has come where the bone should have completely healed.
本周也是欧文在9月16日与班菲尔德的比赛中骨折后的第六周,现在已经到骨头完全治愈的时间了。
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Barack Obama and John McCain are the big winners in yesterday's Mississippi presidential primary. Obama won almost all the black vote but just a quarter of the white vote. He and Hillary Clinton are now focusing on the big delegate count in Pennsylvania where the primary is still six weeks away.
在昨天的密西西比州总统初选中,Barack Obama和John McCain是最大的赢家,Obama赢得了所有的黑人投票但只赢得四分之一的白人投票,他和Hillary Clinton都把目光投向宾夕法尼亚州六周后的初选,因为那里占据不少的选票。
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Barack Obama and John McCain are the big winners in yesterday's Mississippi presidential primary. Obama won almost all the black vote but just a quarter of the white vote. He and Hillary Clinton are now focusing on the big delegate count in Pennsylvania where the primary is still six weeks away.
在昨天的密西西比州总统初选中, Barack Obama 和 John McCain 是最大的赢家, Obama 赢得了所有的黑人投票但只赢得四分之一的白人投票,他和 Hillary Clinton 正在关注宾夕法尼亚州六周后的初选,这里占据不少的选票。
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By March, they'd been back in Fort Wayne six weeks and Nancy was in despair .
到了3月,他们回到韦恩堡已经6个星期了,南希陷入绝望。
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January 13, 2010 - In just about six weeks the Little Corporal will be invading desktops in Creative Assembly's Napoleon Total War .
2010年1月13日-在短短的六个星期的小下士将入侵创新大会的拿破仑全面战争桌面。