查询词典 Simulium

Objective With the help of Primer Premier 5.0 to design PCR primer about simulium COI gene and verify the efficiency and speciality of PCR about these primers.

目的 借助专有软件设计蚋科COI基因的PCR引物，并且检验所设计引物的PCR扩增效率和特异性。

Methods Four pairs of these primers that would be amplified in simulium COI gene were designed by Primer Premier 5.0, the results of PCR would be investigated through agarose gel electrophoresis.

运用Primer Premier 5.0软件设计4对蚋科COI基因的PCR扩增引物，通过PCR扩增后进行琼脂糖凝胶电泳检测实验。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。