查询词典 Sacramento perch

Perch (scientific name: Perca fluviatilis) perch to perch Section species of fish, the common name five black.

河鲈（学名：Perca fluviatilis）为鲈科鲈属的鱼类，俗名五道黑。

This research is taking sea perch as the material, and cloning the Syntaxin 1B gene. After extracting RNA from sea perch tissue, we gained the first chain of cDNA by RT-PCR. Then designing a pair of primers according to the sequence of Syntaxin 1B from other organism. Based on the first chain of cDNA cloned from sea perch, the partial sequence of Syntaxin 1B gene is been cloned by using TD-PCR and we received the 751bp fragment at last.

本实验从鲈鱼脑组织中提取总RNA，通过反转录得到cDNA第一条链，根据已克隆出的其它生物的Syntaxin 1B基因序列，设计并合成引物，在妒鱼syntaxin1B基因部分序列克隆与分析 cDNA第一条链的基础上使用降落PCR，进行体外扩增妒鱼 Syntaxin IB基因的部分序列，得到长度为75 lbP的片断，在胶中洗脱下该片段，与pMD 18一T载体链接，转化入感受态细胞，挑取阳性克隆扩大培养，提取质粒，经HindIH和x一gaH两内切酶消化得到75 1 bp的片断，证明链接成功。

After a nine-for-25 shooting effort in a loss Tuesday to Sacramento, he joked that he probably missed the "cowbells and the food poisoning," references to the rowdy Arco Arena crowd that often harassed the Lakers earlier this decade, as well as his infamous bout with food poisoning from hotel room service in Sacramento.

在周二25投9中输给国王后，他开玩笑说自己可能因为&魔咒和食物中毒&的原因所以全无手感，魔咒是指近十年国王的主场总是会给湖人制造不少麻烦，而食物中毒则指萨那门托臭名昭著的酒店服务。

The EPA and DHA content in the fat of muscle and entrails of 5 varieties freezing sea fishes such as salmon,hairtail,little yellow croaker,blackbluespotted mackerel and silver butterfish, and of 9 varieties fresh water fishes such as river perch,Chinese perch,Chinese sturgeon,silver loweye carp,bighead carp,African crucian carp,bluntsnout bream,common carp and grass carp have been determined.

通过对鲑鱼、带鱼、黄花鱼、鲅鱼和鲳鱼 5种市售海水鱼的肌肉与鲈鱼、鳜鱼、中华鲟、鲢鱼、鳙鱼、罗非鱼、武昌鱼、鲤鱼和草鱼 9种市售淡水鱼的肌肉和内脏脂肪中EPA、DHA的含量的分析比较，结果显示，除鲤鱼和草鱼外，无论是海水鱼还是淡水鱼，其脂肪中均含有一定数量的EPA、DHA。

The fry of black seabreamand giant sea perch with 4.0cm-8.5cm using the methods of dyeing, i.e., the fish immersed in the water solution containing comestible pigment, immersion in tetracycline, i.e., the fish immersed in the water solution containing tetracycline, and marking by spraying, i.e., the fish sprayed with fluorescent pigment spraying on the skin of fish, and double marking, i.e., the fish sprayed with fluorescent pigment spraying on the skin of fish after feed with tetracycline, and control group. The survival rates, mark retention rates and hydrology data were separately measured every three days for black sea bream and every two days for giant sea perch. The results were as follows.

本研究针对体长4.0cm-8.5cm的黑鲷及金目鲈的鱼苗采用染色法(将鱼只浸泡在含有食用色素的水溶液中)、四环霉素浸泡法(将鱼只浸泡在含有四环霉素的水溶液中)、喷枪法(利用高压喷枪将萤光色素打在鱼的表皮)以及双重标识法（四环霉素投餵法加上喷枪法）与一组空白组进行试验，黑鲷每三天一次、金目鲈每两天一次，调查标识后鱼只的时间别残存率、时间别标识残留率以及水质资料，结果如下。

The different fishes which are employed in the present studies are wild-type salmon, cultured salmon of freshwater and seawater, sea perch and fat greenling. The complete CT gene sequences of salmons are obtained by PCR amplification. The partial CT sequences of sea perch and fat greenling are obtained by in vitro cloning PCR method. Alignment of obtained CT sequences with other fish CT shows that CT appears to be well conserved among the same family. And the relative in taxonomy is far away, the similarity of different fish CTs is low. On the contrary, the closer the relative in taxonomy is, the higher the similarity of different fish CTs is. The sCT is expressed in pGEX-4T-X by recombinant form . We also succeed in the research of sCT expression alone by expression PCR. In addition, sCT antiserum is obtained using GST-sCT as antigen, and the high titer is tested by double immunodiffusion. In the rat bioassay, administration of 50 μg recombinant protein evoked significant hypocalcemia at 1 h after the data are analyzed by t-test.

本文用PCR方法克隆了野生鲑鱼、养殖鲑鱼的降钙素基因，并应用体外克隆PCR的方法首次克隆出鲈鱼、六线鱼降钙素的部分基因序列，通过对克隆的降钙素序列的比较研究，结果显示同一科的鱼降钙素序列保守性较高，同时，根据降钙素的部分氨基酸序列进行了降钙素相似性的比较研究，结果显示在分类学上，分类地位较远的鱼，其降钙素相似性较低，分类地位越接近的鱼，其降钙素相似性越高；我们利用谷胱甘肽S-转移酶(Glutathions S-transferase，GST)融合表达载体pGEX-4T-X对克隆的鲑鱼降钙素基因进行了融合表达研究，应用表达PCR的方法对降钙素基因的独立表达进行了初步的研究探索，并将纯化的融合蛋白作为抗原，获得了高效价的兔抗鲑鱼降钙素免疫血清；生物活性研究表明，大肠杆菌表达的融合蛋白具有显著的降血钙作用。

The perch swallows the grub-worm, the pickerel swallows the perch, and the fisher-man swallows the pickerel; and so all the chinks in the scale of being are filled.

鲈鱼吃了螬蛴，梭鱼吃了鲈鱼，而渔夫吃了梭鱼；生物等级的所有空位就是这样填满的。

The endurance abilities of the embryos of sea perch, flounder and turbot at different developing stages to vitrification solutions were studied. The result showed that sea perch at tail-bud stage, heart-beating stage and pre-hatching stage had more endurance abilities to VSD2, flounder at 4-5 somites, 16-20 somites and tail-bud stage had more endurance abilities to PM3, and turbot at 4-5 somites, 16-20 somites and tail-bud stage had more endurance abilities to PMP1. The feasible equilibration time of three marine fish embryos in vitrification solutions was determined, which guided the dealing process before freezing.

对花鲈、牙鲆、大菱鲆三种鱼类不同时期胚胎分别在玻璃化液中的适应性进行了研究，发现花鲈尾芽胚、心跳胚和出膜前期胚对VSD2的耐受能力较强；牙鲆4-5以肌节胚、16-20对肌节胚、尾芽胚对PM3的耐受能力较强，大菱鲆4—5对肌节胚、16—20对肌节胚、尾芽胚对PMP1的耐受能力较强，较适合于进行玻璃化液处理和冷冻保存；并且确定了三种鱼类各时期的胚胎在玻璃化液中的适宜平衡时间，为胚胎在玻璃化液中处理和冷冻提供了一定的参数。

In 1991, frozen squirrels were used to bludgeon a woman in Sacramento, California.

1991年，在加州，冷冻的松鼠被用棒击一个妇女。K.K.S，老挝移民，重复的用6个冷冻的松鼠击打他的妻子，直到这个可怜的女人不省人事。

On November 25, 1849, he wrote a letter to his sister Mary about his experiences in the boomtown of Sacramento City.

在1849年的11月25日，他写了一封信给他的姊姊玛丽，说明他在沙加缅度这个新兴都市的各种经验。

"Bird Man's Diary, Butting on the Love Island", Changsha, China

2008年"鸟人日记，顶在爱情岛"，中国长沙

As long as they were in the service of a family of wizards, their magic would remain dormant.

只要他们一天为家庭服务，他们的法力就潜藏不动。