查询词典 SSR

With 16 hybrids and their parents and 202 skeleton inbred lines as the primers for screening materials, the artificial groups of 2 standard diad and 2 standard triplet were build resp. as the verification materials. Genomic DNA of seedlings was extracted by using CTAB method. 137 pairs of SSR primers were selected for SSR amplification and product detection, which was used in the parentage identification of maize varieties.

方法］以16个玉米杂交种及其双亲和202个骨干自交系为引物筛选材料，分别构建2个二联体亲子鉴定盲样和2个三联体亲子鉴定盲样，用CTAB法提取幼苗叶片DNA，选137对SSR引物进行玉米品种的亲子检测。

The purpose of the research is: 1 to make the distinguishment of these hybrid rice combinations and their parents; 2 to establish a set of screening pictures and DNA fingerprint map concerning these super hybrid rice combinations and their parents; 3 to find a convenient method for identification of rice seed purity.In this study, 144 SSR primer pairs distributing in 12 rice chromosomes were used to detect the experimental materials.

本研究通过SSR分子标记技术分析进行超级杂交稻先锋和苗头组合的鉴定，获得以下结果：(1)对分布于水稻1-12号染色体的144对SSR引物进行了筛选，有47对能够在实验材料中显示较好的多态性通过，占检测引物的32.6％。

In an effort to simplify the procedure and to reduce the cost of fluorescence SSR analysis,the conditions of the multiplex PCR and the multiplex gel electrophoresis were optimized in the genetic analysis of sunflower Helianthus annuus L.

在向日葵(Helianthus annuus L。)自交系SSR遗传分析中，为了提高SSR荧光分析效率、简化分析程序和降低研究费用，我们进行了多聚PCR和多聚凝胶电泳两项技术的优化研究。

22 Of 22 pairs EST-SSR primers from Chinese cabbage/rape respectively had amplification products in soybean,wheat and maize,the rate of amplification was 81.8%,95.4%,100%,respectively;10 of 22 pairs EST-SSR primers showed polymorphism in monocotyledons wheat and maize,13 in dicotyledons oilseed rape and soybean,7 in both monocotyledonous and dicotyledonous crops.

（1）30对小麦EST-SSR引物中，有29，28和26对引物分别在玉米、油菜和大豆中有扩增产物，可扩增率分别为96.7%，93.3%和86.7%；其中分别有12对和9对引物在单子叶作物小麦和玉米及双子叶作物大豆和油菜中的扩增产物显示多态性，有4对引物在4种作物中的扩增产物均显示多态性。

Ofeffective 1st branches, length of main inflorenscence, effective siliques of maininflorenscence, density of main inflorenscence, length of silique, seed per sillique,1000 seed weight, total effective siliques per plant had significant positive correlationwith yield per plant. Polymorphisms between zhongshuang No.4 and H228 was examined by SSR primers. 152 primers Showed polymorphisms, and a linkage map containing 123markers, including 18 linkage groups was constructed. The total genetic distance ofthe map was 3483.1cM, and the average distance of two adjacent markers was28.32cM.

选用SSR引物对中双4号和H228间进行多态性检测，有152对引物在双亲间表现多态性，用这些引物分别对142个株系进行检测，构建了含有123个SSR分子标记、18个连锁群的连锁遗传图，总图距为3483.1cM，标记间平均距离为28.32cM，各标记在18个连锁群上的分布很不均匀，其中，第1、12、13、17连锁群上分布标记较多，其它连锁群上分布标记较少。

Among the 187 pairs of SSR primers, 73 (39%) were polymorphic between the two parents and were used to tag submergence in the F2 population.

在SSR分析中， 187对SSR引物中有73对引物在两亲本间有明显的差异，差异率为39%。

Four hundred and twenty three ( 262 for HB×T, 49 for S×T, 111for R×T and 1 for B×T) out of 990 individuals exhibiting the trifoliate phenotype oftrifoliate orange were considered to be hybrids by morphology identification and SSRverification; Six hundred and fourteen of 756 plantlets derived from other crosses wereverified to be hybrids by SSR analysis. Of them, 244 for HB×\'Nova\' tangelo, 179 forHB×\'Page\' tangelo, 172 for HB×\'Fairchild\' tangelo, 1 for \'Bendizao\'tangerine×\'Nova\'tangelo, 7 for \'Bendizao\'tangerine×\'Page\' tangelo, 3 for \'Bendizao\'tangerine×\'Murcott\'tangor and 8 for satsuma mandarin×\'Nova\' tangelo.

为区分杂种苗和珠心苗，用形态识别和SSR分析，鉴定了990株枳的杂交后代，得到了423株三叶杂种(HB柚×枳262株，温州蜜柑×枳49株，红橘×枳111株，华农本地早橘×枳1株)；SSR分析鉴定了其它杂交后代的756个单株，结果共获得了614株杂种，其中HB柚×诺瓦244株，HB柚×佩奇179株，HB柚×费尔切尔德172株，华农本地早橘×诺瓦1株，华农本地早橘×佩奇7株，华农本地早橘×默科特3株，温州蜜柑×诺瓦8株。

Using RAPD markers techonolgy analysed the genetic structure of populations and built up the reaction system of SSR markers of Aristida pennata, at the same time, analysed the genetic structure based on SSR markers techonolgy. The research results are as follow:(1) 11 amplifiction random primers were used to detecte 125 loci in 76 samples. The total proportion of polymorphic loci of. Aristida pennata is 96.8%, the average proportion of polymorphic loci of Aristida pennata is 45.3%. The analyses of Shannon diversity index (1,0.5151) and Nei gene diversity index(h, 0.3471) indicate that the gene variation is rich in subpopulations.

Pennata.tirn为研究对象，在阜康，147团及121团周边等7个亚种群采集76个样品，通过RAPD分子标记技术进行亚种群内和种群间的遗传变异分析，并采用SSR技术建立羽毛三芒草的检测技术平台同时进行遗传结构分析，结果表明：(1) 11条RAPD引物检测到76个样品有125个位点，总多态位点百分比为96.8％；亚种群内平均多态位点比为45.3％，香农表型多样性指数(1，0.5151)，Nei's基因多样度指数(h，0.3471)都说明羽毛三芒草种群有较为丰富的遗传变异。

A common carp gynogenetic line including 44 individuals derived from the cross Barbless carp Hebao-cold tolerance red carp was used to construct a linkage map using 445 markers (265 AFLP markers, 127 SSR markers, 37 EST-SSR markers and 16 RAPD markers).

用265个AFLP标记、127个微卫星分子标记、37个EST-SSR标记和16个RAPD标记对大头鲤/荷包红鲤抗寒品系的F2雌核发育群体44个个体进行基因型检测，构建鲤鱼遗传连锁图谱。

The result of SSR analysis supported that the species status of Changshou kumquat; the Ningbo kumquat, Rong' an kumquat and Lanshan kumquat are the same species of Fortunella; some of the Citrus SSR markers can be used in Fortunella.

用NTSYS-PC软件进行分析，结果表明，金柑属植物与宽皮柑橘的亲缘关系很近；SSR聚类结果支持长寿金柑种的地位；支持宁波金弹、融安金弹、蓝山金柑均为金弹的栽培种的结论。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。