查询词典 SSR

One disease resistance segregating population 3B789, derived from "EM85/Torfrida//Wen6/3/3*Jing411/4/Nongdal89", was selected for SSR analysis using BSA approach. An EST-SSR marker Xcau33 was found to be co-segregated with the resistance gene Pm4b in a 231 plants population and could be used for marker-assisted selection of both Pm4a and Pm4b locus.Forty accessions of wild emmer T. turgidum var.

利用一个来自杂交组合"EM85/Torfrida∥温6/3/3~*京411/4/农大189"的抗病分离群体3B789，通过BSA法建立SSR标记，发现在分离群体3B789的231个单株中，EST-SSR标记Xcau33与抗病基因Pm4b表现共分离，能够在辅助选择Pm4a和Pm4b基因的育种工作中应用。

The DNA fingerprints of 4 biotypes of GM population from 7 locations were analyses by AFLP.Base on the fine mapping of resistance gene Gm6 against all 4 Chinese GM biotypes by RAPD and SSR methods respectively,the STS markers and SSR markers linked to gene Gm6 were used for breeding through marker assisted selection.Some new resistant germplasm with Gm6 gene were created.And 6 cultivars and 6 lines of two-line hybrid rice and 1 line of three-line hybrid rice against GM were bred and extended to the farmer.The technique system of MAS for resistant varieties against Asian rice gall midge was set up at Guangdong province of China.

用AFLP方法对从中国广东省7个地点采集的4个生物型的DAN指纹进行分析；在对用RAPD和SSR技术分别对抗中国4个稻瘿蚊生物型的基因Gm6精细定位基础上，用与Gm6紧密连锁的STS和SSR标记开展分子标记辅助育种，创造了一批抗稻瘿蚊的新种质，包括育成了6个栽培稻和6个二系杂交稻和1个三系杂交稻并在农户试种，在中国广东成功地建立了分子标记辅助选育抗稻瘿蚊品种的技术体系。

(1)29,28,26 of 30 pairs of EST-SSR primers from wheat respectively had amplification products in maize,oilseed rape and soybean,the rate of amplification was 96.7%,93.3%,86.7%,respectively;12 of 30 pairs EST-SSR primers from wheat showed polymorphism in monocotyledons wheat and maize,9 in dicotyledons oilseed rape and soybean,and 4 in both monocotyledonous and dicotyledonous crops.

基金项目：国家生命科学与技术人才培养基地科技创新基金项目(2007088)；国家油菜产业化项目科学家岗位"化杀岗位"（nycytx-00505）；国家自然科学基金项目(30871578)中文摘要：探讨单、双子叶作物间EST-SSR引物和标记的通用性。选取30对小麦、8对油菜和14对白菜的EST-SSR引物，分别以10个小麦、10个油菜、5个大豆品种和5个玉米自交系的基因组DNA为模板，进行PCR扩增及扩增产物的多态性分析。

The plentifulness of SSR polymorphism in silkworm genome and the differences of the polymorphism among different silkworm races indicate the advantage of SSR in the construction of silkworm DNA fingerprints and analysis of inheritance diversity, and show the foreground of application of SSR in silkworm molecular marker technology.

家蚕基因组丰富的SSR多态性和不同家蚕品种之间的SSR多态性差异，表明了SSR标记在遗传多样性分析中的优势，展示了SSR分析在家蚕品种分子标记技术中的良好应用前景。

Eighteen SSR primer pairs were designed and synthesized.The factors which affected on the SSR reaction of Toona ciliata var.

利用所合成的引物优化SSR反应体系，对影响SSR反应的各个因子进行了探讨。

Using large sample DNA of capacity technology, the maize high lysine content of SSR mark analyse technological platform has been established in this paper. The main results are as follows: the five comparing research methods of drawing maize DNA mark have shown that the method of iso- sulphur cyanic acid guanidine drawing maize genome DNA has the superiority of using less medicine, operating sequence more simple , faster, and costs lower , steady and reliable quality of DNA, etc..Utilizing this method , one person can draw 300-400 DNA samples each workday.lt indicates that the method can be used in SSR marker of maize,.

本文从大样本容量的DNA快速提取技术入手，初步建立了玉米高赖氨酸含量的SSR分子标记分析技术平台，获得的主要结果如下：对五种玉米DNA提取方法的比较研究认为，异硫氰酸胍法提取玉米基因组DNA，具有使用药品少、操作步骤简单、快速、成本低、DNA质量稳定可靠等优点，利用该方法，每人每个工作日可以提取300～400个DNA样品，可以在玉米SSR标记分析中应用。

The major results are as follows: 1 Types of tandem repeats are rich in the EST of the Haliotis discus hannai; 2 Sequences annotated by Gene Ontology have few types of SSR; 3 ESTs have a wide distribution, which is helpful to SSR marker study.

分析结果表明：(1)皱纹盘鲍EST中串联重复序列类型丰富：(2)皱纹盘鲍EST Gene Ontology注释序列SSR类型单一；(3)皱纹盘鲍EST-SSR分布广泛富，是EST-SSR标记开发的优良资源。

In the study, SAM (selectively amplified microsatellite) was used to isolate the SSR locus of Setaria italica and in all 163 clones containing SSR sequence were obtained, of which 106 were unique SSRs, accounting for 65%.

首次将SAM法用于谷子SSR位点的分离，共获得163个含有SSR序列的克隆，其中unique SSR106个，占所有SSR序列的65%，60个SSR序列的3'端成功设计特异引物，占unique SSR的56%。

Microsatellite loci sequences of family Trichogrammatidae parasitoid wasp from Genbank were searched, and four microsatellite primer pairs that adapt to T.dendrolimi SSR analysis were screened.The PCR reaction conditions of microsatellite primers for T.dendrolimi were tested , and SSR reaction system of polymophic test for T.dendrolimi population genetic structure was established.The SSR molecular marker create new method for genus Trichogramma parasitoid wasp study.

在搜索Genbank中主要赤眼蜂属寄生蜂及与赤眼蜂亲缘关系相近寄生蜂微卫星座位序列信息的基础上，筛选了可以用于松毛虫赤眼蜂SSR分析的4对微卫星引物，摸索PCR反应条件，建立松毛虫赤眼蜂种群群体遗传结构多态性检测的SSR指纹标记体系，SSR分子标记技术为赤眼蜂属寄生蜂研究提供了新方法。

The ISSR fingerprinting of 18 Japanese plum cultivars and 12 plum species or variety were established by using optimum system with primer 5"-9T-3"and 5"-9C-3", respectively. The identification rate of plum cultivars and species or variety was 100%. The effects of five important components on reaction of SSR were studied with Prunus salicina cv. Meilili. The results showed that the optimum concentration of five important components i.e. Tag DNA polymerase, Mg +, single primer, template DNA and dNTPs in 25 μL reaction system of SSR were 1.5u, 2.0mM, 0.8 μ M, 30~40ng and 0.16~0.24mM, respectively. The dendrograms of these cultivars was contructed according the Jaccard coefficient of similarity.

利用该优化体系，以5′-_9T-3′为引物，构建了中国李18个品种的ISSR指纹图谱，该引物可将这些品种完全区别开来；以5′-_9C-3′为引物，构建了李属6类种质资源的ISSR指纹图谱，该引物区分率为100％。4、中国李品种适宜的SSR反应分析体系是：25μL SSR反应体系中，Taq DNA聚合酶、Mg~(2+)、每个引物、模板DNA和dNTPs等5种成分的适宜浓度分别是：1.5u、2.0mmol·L~(-1)、0.8μmol·L~(-1)、30～40ng和0.16～0.24mmol·L~(-1)。5、采用RAPD、ISSR和SSR等三种分子标记技术对中国李品种资源54份和2个欧洲李品种进行了遗传多样性分析，并以品种间的遗传相似系数构建了Jaccard聚类树状图。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。