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Using RT-PCR and RACE methods,5′ up stream and 3′down stream cDNA sequence of ABP1 were isolated from Gossypium hirsutum for the first time.

测序及生物信息学分析表明，该基因cDNA全长824 bp编码190个氨基酸，它所编码的氨基酸序列包含植物ABP1蛋白家族的所有特征，被命名为GhABP1。

In this study, an attempt is made to account for the observed difference in the susceptibility of Gossypium hirsutum L.(cv. BD-18 and Simian 3, resistant and susceptible to V. dahliae, respectively) to toxin of Verticillium dahliae, by looking for differences in the timing and level of production of NO and H_2O_2, and their effects on the expression of GST gene.

本实验以棉花抗病品种BD-18和感病品种泗棉三号的悬浮细胞为材料，研究了棉花细胞在黄萎病菌毒素处理后H_2O_2和NO的变化，并用半定量RT-PCR的方法检测毒素、H_2O_2和NO对棉花谷胱苷肽-S-转移酶基因转录水平的影响。

Result: the regions with strong to very stong labeling by the GDNF probes were as follows: the caudate-putamen, the habenular nucleuse, and some areas of the brainstem and spinal cord, the trigeminal ganglion, the dorsal root nerve ganglion.

本实验利用原位杂交技术结合RT-PCR技术观察了GDNF从E16～成年大鼠神经系统内的分布；为了更进一步研究GDNF在神经发育中的作用与作用机制，本实验还利用基因克隆技术克隆了人GDNF基因并进行了原核表达GDNF蛋白的研究。

Methods Both a rat model with habenular lesion and a hypnotic-treated rat model were established by passing a DC current or by infusing with zolpidem into the stomach and then the rats were divided randomly into habenular sham group,habenular lesion group,vehicle group and zolpidem group.The mRNA levels of adenosine and 5-HT-related genes were examined by RT-PCR in the PO/AH.

以30只雄性Wistar大鼠为实验动物，直流电损毁缰核或灌胃给予催眠药唑吡坦建立缰核损毁大鼠(n=15)和唑吡坦处理大鼠(n=15)两种动物模型，随后随机将大鼠分为缰核假手术组(n=7)、缰核损毁组(n=8)、生理盐水对照组(n=7)和唑吡坦处理组(n=8)。

Real-time quantitative pcr, immunohistochemistry, dry-wet weight method, histological techniques and haematoxylin and eosin stain were used to detect expression of icam-1, the change of mrna at different time phases, water containing in the brain tissue and the inflammatory infiltration respectively after diffuse brain injury.

采用marmarou方法获得大鼠弥漫性脑损伤模型，实时定量rt-pcr、s-p免疫组化法分别测定icam-1蛋白和mrna在外伤后不同时间点的表达变化，干湿重法测脑组织含水量，组织切片苏木精-伊红染色观测炎性细胞浸润情况。

Methods The rat models of diffuse brain injury were established by Marmarou's method. Real-time quantitative PCR, immunohistochemistry, dry-wet weight method, histological techniques and haematoxylin and eosin stain were used to detect expression of ICAM-1, the change of mRNA at different time phases, water containing in the brain tissue and the inflammatory infiltration respectively after diffuse brain injury.

采用Marmarou方法获得大鼠弥漫性脑损伤模型，实时定量RT-PCR、S-P免疫组化法分别测定ICAM-1蛋白和mRNA在外伤后不同时间点的表达变化，干湿重法测脑组织含水量，组织切片苏木精-伊红染色观测炎性细胞浸润情况。

The key research progresses and results that we accessed by the project were as follows: 1 A pair of degenerated primers were designed in the conserved domain which based on the amino acid sequence of HAK in Arabidopsis thaliana. The 500 bp fragment of HAK were amplified from the roots of Puccinellia tenuiflora by RT-PCR. The full sequence of HAK were obtained by 5' and 3' RACE methods and were determined and analyzed.

本项目主要取得了以下研究进展和成果：（1）根据已有HAK转运蛋白氨基酸序列在保守区域设计简并PCR引物，用反转录PCR方法，扩增出500bp的小花碱茅根HAK片段，通过5''和3''RACE的方法获取了该HAK的全长序列，完成了测序和序列同源性分析。

METHODS Sixty-five cases of T3NOMO glottic squamous cell carcinoma treated with curative intent by three different modalities include vertical hemilaryngectomy(VHL, n=21), total laryngectomy (TL, n=31) and radiotherapy(RT, n=13) were reviewed retrospectively.

分析1992年～1999年我院采用喉垂直部分切除术、喉全切除术和根治性放射治疗等方案治疗的65例喉声门型癌T3NOMO病变的临床资料，比较不同治疗的总体生存率、原发部位及颈部淋巴结复发率和喉功能保留率。

Fibroblasts have the ability to secret many kinds of extracellular matrix and cytokines relevant to hepatogenic differentiation. So, we investigate whether coculture of hADSCs with fibroblasts without cell-cell contact play a role in hepatic differentiation of hADSCs by RT-PCR analysis of hepatic specific gene expression and real-time PCR analysis of Alb expression.

成纤维细胞能够分泌很多利于干细胞向肝细胞分化的细胞外基质和细胞因子，我们采用非接触共培养的方式对成纤维细胞与人脂肪干细胞共培养能否引发其向肝细胞的分化进行了研究。

The renal function and histopathological changes were evaluated at 1,2,4,8,12,16,26 and 40 weeks after operation finished.

在1、2、4、8、12、16、26和40周，用原位末端标记法、免疫组化、RT-PCR、Western—blot分别检测细胞凋亡、caspase-3，-8，-9的mRNA和蛋白质的水平。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。