查询词典 RT

Methods The mRNA level of tissues of 39 cases of breast cancer and 14 cases of fibroadenoma was measured by RT-PCR technique and its correlation was analyzed.

抽取乳腺肿瘤组织总RNA，采用半定量逆转录－聚合酶链反应检测39例乳腺癌和14例乳腺纤维腺瘤组织中RECK-mRNA和MMP9-mRNA的表达，分析其关系。

The 7.4 kb DNA fragment containing the S. Choleraesuis std fimbrial gene cluster was PCR amplified and cloned into the pCR-XL-TOPO vector. The open reading frames of the std fimbrial gene cluster were analyzed. The resulting recombinant DNA was transformed into Escherichia coli HB101 strain.

本实验室藉由PCR方式，增幅7.4 kb的std线毛基因组，将其选殖至pCR-XL-TOPO质体DNA，委外定序后，分析std线毛基因组之开放性读框，进而将此重组DNA送入大肠杆菌HB101，并藉由RT-PCR及西方点墨试验观察到S。

To investigate the function of STT3a gene in salt adaptation and flagellar regeneration of Dunaliella salina, a pair of degenerate primers was designed according to conserved homologous amino acid sequences of VCVFTA and DVDYVL of STT3a from Chlamydomonas, Arabidopsis thaliana and other organisms. A cDNA sequence of 1 650 bp encoding a whole functional domain of STT3a was amplified from D.

为了研究STT3a基因在杜氏盐藻耐盐及鞭毛再生方面的作用，根据衣藻、拟南芥等STT3a蛋白的氨基酸高度保守序列VCVFTA、DVDYVL设计一对简并引物，采用RT-PCR及3'RACE的方法扩增杜氏盐藻STT3a蛋白功能结构域的cDNA序列。

METHODS: The expression of GATA- 3 gene was analyzed by using RT - PCR - ELISA in BMSCs from 34 normal cases and 9 cases with AA.

目的：了解转录因子GATA-3基因在再生障碍性贫血和正常骨髓基质细胞中表达的情况，探讨AA发病的免疫机制。

However, the binding patterns between the HU-induced and uninduced HEL cells were different. The data in Southwestern-blot analysis showed that the molecular weights of the GATA factors binding to the HS2core DNA sequence were 43kd and 47kd respectively.

反义寡核苷酸技术及RT-PCR的结果表明，GATA-1的反义核苷酸抑制了羟基脲诱导的β-珠蛋白基因的表达，进一步证明了GATA-1因子在羟基脲诱导的HEL细胞分化过程中是不可缺少的促进因素。

The expression of Gekko Fstl1 was analyzed by northern blot, in situ hybridization and reverse transfer polymerase chain reaction.

通过RT-PCR方法研究Fstl1基因在壁虎主要组织中的表达分布与壁虎断尾损伤模型中变化。

METHODS: RT-PCR was used to observe the expressions of AT2 mRNA in the cortex of rat kidney, including neonate rats (1 d), mature rats (8 weeks) and gerontic rats (20 months).

分别观察新生(1d)、成年(8wk)及老年(20mo)3组大鼠肾脏皮质中AT2mRNA的表达及其增龄性变化，并测定肾皮质中AngⅡ水平。

Methods MTT assay for detection of single use of Adriamycin and combination of Adriamycin with gleditsia saponin on the Role of the SMMC-7721 cell survival rate; detection of mRNA expression of bcl-2 by PCR method; detection of protein expression of bcl-2 by Western blotting method.

MTT法检测单独应用阿霉素或阿霉素与皂角皂苷联合作用的SMMC-7721细胞的生存率；RT-PCR检测bcl-2 mRNA表达；Western blotting检测bcl-2蛋白的表达。

Objective To evaluate the effects of glomerular cell proliferation and apoptosis in experimental glomerulosclerosis.

用RT-PCR方法和免疫组化法检测增殖细胞核抗原的表达，原位检测肾小球细胞凋亡指数。

Methods: hPDLCs of passage four were treated with 0.1% absolute ethyl alcohol, 10^(-10)mol/Lor 10^(-7)mol/L E, in DMEM supplemented with 10% FBS, 10mM β-glycerophosphate and 5μg/ml ascorbic acid for 24 hours, 48 hours and 72 hours.

采用剂量对照设计，评价四代hPDLCs分别在含有0.1%无水乙醇、10^(-10)mol/L E2或10^(-7)mol/L E2的矿化诱导培养基中培养至72h，时间点的观察荧光定量RT-PCR检测RANKL和OPC mRNA的表达水平。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。