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Hematoxylin and eosin, Alcian blue, Masson Trichrome, and Elastica Van Giesonstains were performed for each LF. Nine LF were collected and were used for biologic study of real time RT-PCR to quantitatively measure mRNA expression of Type I collagen and elastin in each LF.

每份标本用HE染色、Alcian蓝染色， Masson Trichrome染色以及Elastica Van Gieson染色。9份标本染色后收集，用实时定量PCR定量分析每份标本中I 型胶原纤维和弹力纤维的mRNA的表达。

Elative quantitative RT-PCR was used to determine the expression of MDR1、GST-π、p53 mRNA in COC1 and COC1/DDP cells after DDP administered 24 hours.

T-PCR半定量法测定不同浓度DDP作用于COC1及COC1/DDP细胞24h后P53、GST-π、MDR1mRNA含量的变化。

The RNA genome of virus used forRT-PCR can be isolated not only from the infected chicken embryonated fluids, but also directly from tissue homogenate of field samples. The detection results of 67 field samples were identical with that of the conventional diagnosis.

本方法只需一个RT-PCR反应，整个过程在8小时内完成，既可取感染鸡胚尿囊液检测， 2002年）丫H人《'WIf寸它义：也可从病料直接检测。

In order to develop antibacterial peptide biologics for prevention and control of endometritis of dairy cow,a pair of primers was designed and synthesized according to the sequence of bovine antibacterial peptide gene published in GenBank.The total RNA was purified from the dairy cow endometrial cells with the Total RNA Isolation Kit.

为研发防治乳牛子宫内膜炎的抗菌肽生物制剂，根据已发表的牛抗菌肽基因序列设计了1对引物，用试剂盒提取乳牛新鲜子宫内膜总RNA，经RT-PCR扩增，将克隆的抗菌肽BNBD5基因全长编码区的cDNA连接到原核表达载体pET30a中，经IPTG诱导原核表达，通过Western-blot检测融合蛋白BNBD5的表达水平。

Taking mRNA from ephebic Bombyx mandarina as templates,the cDNA of four troponin I isoforms was cloned by RT-PCR,named TnI-A,TnI-B,TnI-C and TnI-D(GenBank accession numbers:FJ785828,FJ785829,F3785830 and FJ890515 )respectively.Sequence homology comparison and functional prediction of the amino acids encoded by the four TnI gene isoforms in Bombyx mandarina indicate that they have characteristics of troponin families.

与电子克隆到的相应的家蚕TnI亚型比较分析，结果显示：野桑蚕与家蚕TnI-B有相同的氨基酸，TnI-A和TnI-C有一个氨基酸的差别，而在家蚕中没有电子克隆到与野桑蚕TnI-D相同的亚型。3。

The results of semi-quantitative RT-PCR showed that the expression of RAR1, SGT1 both increased in powdery mildew resistance Haynaldia.villosa leaves when inoculated by powdery mildew Erysiphe graminis D.

对条锈菌诱导后的抗病材料92R137、感病材料扬麦5号进行半定量分析，结果表明在抗、感材料中RAR1基因都受诱导，均在24h时表达量达到最大，随后表达量下降。

Human erythropoietin receptor plays an important role in regulating the red blood cell production by promoting the proliferation and differentiation of RBC from erythroid precursors.

以人胎肝为材料，通过RT PCR的方法扩增出人促红细胞生成素受体的胞外区基因。

Objective:To establish a competitive reverse transcription-PCR assay for quantification of uncoupling protein mRNA and determine UCP mRNA expression levels in intra-and extraperitoneal adipose tissues of adult humans.

目的：建立解偶联蛋白 mRNA表达水平的竞争性RT-PCR测定法；并应用该方法测定UCP mRNA在成人腹膜内与腹膜外脂肪组织的表达水平。

At present,these sandstones are at the late A period of the early diagenetic stage（Ro=0.8%,T=70℃-90℃）,and their diagentic fabrics are controlled by the acidic diagenetic environment, resulting in widespread dissolution of feldspathic grains and well developed quartz overgrowth.

砂岩目前处于早成岩阶段B期的晚期，Rt，为0.8％，温度在70℃～90℃，成岩组构特征受酸性成岩环境控制，因此长石类易溶颗粒普遍发生溶蚀作用，石英次生加大发育。

Objective To establish a method for the determination of ferulic acid in Hongyao tablets by RT-HPLC.

目的 建立红药片中阿魏酸的含量测定方法。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。