查询词典 RT

Methods The t-PA mRNA in peripheral blood nucleared cells was detected by RT-PCR and the t-PA activity was determined by chromogenic substrate method.

用逆转录-PCR法检测外周血有核细胞中t-PAmRNA的表达，发色底物法测定血浆t-PA的活性。

Methods; In APS treatment group and control group, levels of insulin, C-peptide, myocardial enzymes, glycosylated serum protein and angiotensin were measured; Expression levels of chymase and angiotensin-coverting enzyme mRNA were detected by RT-PCR.

检测APS治疗组和对照组DM仓鼠的胰岛素、C肽、心肌酶谱、糖化血清蛋白和血浆。

Activities of chymase and ACE were tested by radioimmunological method. Expressions of phosphoric extracellular signal regulated kinase 1/2 (p-ERK1/2) in hearts were measured by western blot. Results: As compared to the control group levels of GSP, myocardial enzymes and myocardial Ang Ⅱ were much lower in those of APS group.

心肌组织的血管紧张素Ⅱ含量；RT-PCR法检测心肌糜酶和血管紧张素转换酶 mRNA表达；放免法检测chymase和ACE的活性；免疫蛋白印迹法检测心肌磷酸化的细胞外信号调节激酶1/2(p-ERK1/2)含量。

The effect of Sco on expression of RyR mRNA level of TSMC from asthmatic guinea pigs The presence of RyR mRNA was tested by RT-PCR using primer sets based on the 3'codding regions of the RyR genes.

说明哮喘组豚鼠TSMC上RyR2 mRNA表达明显降低，与对照组比较有显著差异（P＜0.01）；Sco组豚鼠TSMC上RyR2mRNA表达情况较哮喘组增强（P＜0.05）。

Objective: To contructre combinant plasmid pET28a/hES and coexpress human Endostatin and predhPK-5 in E. coli. Methods: mRNA from human liver tissue was exracted, and the Endostatin gene was amplified by RT-PCR.

目的构建人内皮抑素原核表达质粒pET28a/hES，并与简化人纤溶酶原饼环区(predigested human PlasminogenKringle5，predhPK-5)在大肠杆菌中实现共表达。

Methods The yield of combinant human IL-18 expression in E.coli remains quite low because the sequence of mature hIL-18 has 37aa rare condons for E.coli in a total of 157aa.

设计特异性引物，以RT-PCR从外周血单个核细胞中扩增出人白细胞介素18成熟肽片段，克隆进入PBV220载体。

The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2 -chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

Based on these previous work, in this research, in order to explore the the molecular mechanism of enhanced tumor cell immunogenicity treated by elemene and mytomycin C compositely, we used murine Hca-F ascitic hepatic carcinoma ( high lymphatic metastasis substrain of H22) as an experimental model to compare the immunoprotective effects on Hca-F cells as well as the mechanism of the effects caused by HSP70-peptide complex purified from untreated tumor cells, EC-TCV and BCG; We studied the mechanism of increased tumor cell membrane HSP expression treated with elemene by the techniques of RT-PCR and immunoelectron microscopy; In order to deeply elucidate the antitumor effects and the molecular mechanism of elemene in increasing tumor cell immunogenicity we analyzed and compared the effects of elemene and heat shock on the gene expressions of tumor cell using gene expression profile chip.

本论文在上述研究成果的基础上，选择小鼠肝癌H22的高淋巴道转移亚系Hca-F为模型，进一步比较了未处理肿瘤细胞来源、榄香烯复合瘤苗来源的和卡介苗来源的HSP70-肽复合物对Hca-F的免疫保护效应、效应机制，探讨榄香烯-丝裂霉素复合处理增强肿瘤细胞免疫原性的分子机制；以Hca-F和人肝癌细胞株HepG〓等为模型研究了榄香烯处理增强肿瘤细胞膜热休克蛋白表达的机制；以HepG〓为模型用表达谱基因芯片研究比较了榄香烯与热休克处理对肿瘤细胞基因表达的影响，从而进一步阐明榄香烯抗肿瘤作用及增强肿瘤细胞免疫原性的分子机制。

Linear effect was found between RT and display angles when we changed configural information, which indicated the normalization mechanism of configural information was mental rotation.

人脸特征信息的认知绩效在除180度外的其他角度随偏离零度的角度增加而下降，线性关系显著，180度时认知绩效有上升的趋势。

The cleavage facets on the fracture surface of three-point bending specimens at RT were determined crystallographically in an Electron Probe with the EBSD.

利用电子背散射衍射对合金室温弯曲断口中解理刻面的晶体学取向进行了测定。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。