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In order to investigate the expression of Lrrcl0 during cardiogenesis, an in vitro cardiogenesis model was developed by using P19 cells which underwent differentiation induced by DMSO.

为探讨Lrrc10在心肌细胞分化过程中的表达，采用DMSO诱导P19细胞分化，建立体外心肌发生模型，显微镜观察细胞形态变化，以心α-MHC和β-MHC作为心肌分化的标志，RT-PCR检测分化过程中各基因的表达。

Morphological change of cells was observed by microscope,α-MHC and β-MHC, used as the markers of cardiogenesis expression of genes during the differentiation, were determined by RT-PCR.

结果表明，P19细胞经诱导后形态发生明显变化；α-MHC在诱导分化6d时开始表达，β-MHC在诱导分化8d时开始表达，表明心肌分化成功。

Doetschman TC, Eistetter H, Kemler R, et al. The in vitro development of blastocyst-derived embryonic stem cell lines: formation of visceral yolk sac, blood islands and myocardium ［J］. J Embryol Exp Morph, 1985,87:27～45.［2］ Robbins J, Doetschman T, Jones WK, et al. Embryonic stem cell as a model for cardiogenesis［J］. Trends Cardiovasc Med, 1992,2:44～50.［3］ Moreadith RW, Radford NB.

被诱导的心肌细胞的判定除常规指标外，我们还用了心脏特异性引物β-MHC对不同收缩时间的心肌细胞总RNA进行RT-PCR扩增和RNA斑点杂交，发现该心肌细胞不仅具有正常心肌细胞的特性，而且在分化早期就高度地表达心脏特异性基因。

The specific primers were designed according to published sequences of PRSV CP genes of Chinese Sm strain in Genebank to perform a RT-PCR, total RNA extracted from PRSV-infected samples,Carica papaya L.cv.

根据Genebank发表的PRSV中国Sm株系的外壳蛋白基因序列，设计特异引物，以美中红（Carica papaya L。

Coli.Methods The cDNA encoding CP was amplified by RT-PCR using the mRNA extracted from premature Carica papaya as a template,and inserted into vector pET22-b.

方法从生番木瓜中提取mRNA后进行反转录，得到木瓜凝乳蛋白酶的cDNA，PCR产物克隆到pET22-b载体中，重组质粒转化至大肠杆菌BL21，进行诱导表达及表达产物的检测。

Depending on the aims of investigations, cathodical charging at RT or gas phase charging at elevated temperatures were adopted.

本文研究氢在亚稳β、近β和α+β两相钛合金中的行为和氢对合金的组织结构和相变过程，以及对合金的室温和高温力学性能的影响。

However, it is not known how orthodontic force influences the function of cementoblast and what is the relationship between the function change of cementoblast with root resorption. So we studied the effect of mechanical stimuli on root resoption by examining the expression OPG and RANKL and exploring the related signal transduction pathway in cementoblast. Methods: Cementoblasts OCCM30 were cultured in DMEM with 10% FBS for 48 hours and starved in DMEM with no FBS for 24 hours, then subjected to mechanical strain by four-point bending system with tension and compression stress at 2000μstrain at 0.5Hz frequency. The flow cytometry was used to examine the cell cycle and proliferation activity after 3h, 6h, 12h, 24h loading respectively. OPG and RANKL mRNA were analysed with real time quantitive RT-PCR after the same loading period as FCM.

本课题利用四点弯曲力学加载系统，采用流式细胞术、实时荧光定量PCR、Western免疫印迹等方法，研究体外培养的成牙骨质细胞株OCCM-30在不同力学环境中增殖活性的变化，以及其表达骨保护素和破骨细胞分化因子的mRNA的变化，探讨应力刺激对成牙骨质细胞表达破骨细胞分化调控蛋白的影响，并通过对应力刺激下成牙骨质细胞分裂原活化蛋白激酶家族中的ERK1/2、P38MAPK活性的变化及其与OPG、RANKL基因表达变化关系的研究，对力学刺激调节破骨细胞分化调控蛋白的相关上游信号转导通路进行初步探讨。

Salina by RT-PCR and 3￠ Rapid Amplification of cDNA Ends, which shared homology with Chlamydomonas (48%), Arabidopsis thaliana (50%), Homo sapiens (46%), etc.

序列分析显示克隆的cDNA全长1 650 bp，具有一定保守性，与衣藻、拟南芥和人的相似性分别为48％、50％和46％。

A new wheat virus disease was reported in Jiangsu province, China. Wheat plants infected showed chlorotic or yellow stripes which was continuous or discontinuous on the leaves, the diseased new leaf became yellow or seared and the spike was unseeded.

为了确诊在江苏小麦上新发生的一种病毒病害，采用ELISA、RT-PCR和序列测定等方法对其病原进行了精确鉴定，并对此病的田间发生流行规律进行了调查分析。

The aim are:lTo examine the proliferation ability and potential chondroblast differentiation ;2To find an ideal condition stimulated BMSC differentiate into chondroblast;3To examine the chondroblast proliferation in porous scaffolds and to explore the interaction between cells and materials;4To examine the release of cytokine in vitro.

取健康成人略后上棘处骨髓 5ml，经 percoll液分离后密度梯度离心，10'砌l密度接种培养，观察原代细胞的贴壁、增殖状况，细胞长满瓶底后进行传代培养；传至第三代细胞，重新消化后以500转／分钟轻度离心 5分钟，10V加 l接种，改用化学限定培养基代替完全培养基培养，倒置显微镜观察细胞生长情况及形态变化，甲苯胺蓝染色观察诱导细胞合成细胞外基质QCM）中的蛋白多糖，免疫细胞化学染色检测ECM中＊胶原的蛋白合成，RT干CR鉴定诱导细胞＊胶原mRNA的表达。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。