查询词典 RT

Two vaccines in China results are avirulent sequence. All these results show that the method can differentiate virulent and avirulent,vaccine.

本试验采用RT-PCR结合内切酶分析，有效的避免了这些方法的不足，并且操作简便、快速，可在24～36h内确定分离株的毒力，也减少了散毒和分离样品在处理过程发生交叉污染的可能性。

The expression change of cytokine IL-8 mRNA in the basilar artery was analyzed by RT-PCR.

结果 IL-8mRNA在SAH组第一次注血后第4- 7天升高，14 天趋于正常。

METHODS: Benzhydroxamic acid derivative E was artificially synthesized. The gastric cancer line 7901 was selected to serve as the target cell. The effect of medicine E on tumor cell activity was examined by cell culture technology in vitro. The change of morphology and the ultrastructure after treatment with medicine E was observed with the optic microscope and the electron microscope.

以胃癌细胞株7901为靶细胞，采用体外细胞培养技术评价苯异羟肟酸衍生物E对肿瘤细胞活性的影响，通过光学和电子显微镜对E药物作用后细胞形态学进行观察，用免疫组化和RT PCR方法检测细胞凋亡。

Methods: 1. Leukemia cell lines HL-60、Jurkat、Molt-4 were grown in RPMI medium 1640 supplement with 10% fetal calf serum and 100U/ml benzylpenicillin and 100 U/ml streptomycin sulfate in a humidified atmosphere containing 5% of CO2 at 37oC.

用脂质体将测序正确的重组子转染至白血病细胞株HL-60、Jurkat、Molt-4中，用RT-PCR、Western Blot方法检测iASPP的表达以及用流式细胞仪检测细胞凋亡的变化状况。

The gene expression of c-myc was detected by RT-PCR and Western blotting respectively.

原癌基因c myc参与调控细胞的增殖、分化和凋亡过程，在多种恶性肿瘤细胞中都有很高的表达[1，2]。

Protein and RNA expression were analyzed by Western blotting and RT-PCR, respectively.

蛋白质和RNA表达分别用Western blotting和逆转录PCR技术进行分析。

Different expression of α-tublin on transcription and translation with RT-PCR and Western blotting analysis.

论文采用的实验技术合理，分析统计科学。

ISH probe of NP gene has successfully detected influenza virus infection, and positive signals are present in the bronchial and bronchiolar epithelium or mononuclear inflammatory cells in the necrotic and proliferative areas.

在本研究中利用ISH和IHC之方法检测福马林固定石蜡包埋组织蜡块中病毒核酸与抗原的存在，同时也利用RT-PCR对野外收集的肺脏乳剂进行检测。

The expression of Pax-6 gene in the different stages was demonstrated by RT-PCR (Real - Time PCR):(1) The mRNA of Pax-6 gene expression was positive in Bufo raddei Strauch embryo at 16 stage; and the gene expression was also detected at 20 stage.

2荧光定量PCR结果显示在花背蟾蜍的晶状体再生的第7天Pax-6基因的表达量达到最高，表明此时期Pax-6基因在晶状体再生的过程中起到调控细胞增殖的作用。

ASES is 59% identical to Artemisia cyclase cDNA clone cASC125, 50% identical to epi-cedrol synthase from A. annua, 48% identical to amorpha-4, 11-diene synthase from A. Annua, 39% identical to the 5-epi-aristolechene synthase from tabacco, 38% identical to vetispiradiene synthase from H. Muticus, 41% identical to the δ-cadinene synthase from cotton. The coding region of cDNA was cloned into procaryotic expression vector pET30a and overexpressed in E. coli BL21 (DE3). The cyclase proteins extracted from bacterial culture were found largely in the insoluble protein fraction. The tissue specific expression patterns were analyzed by RT/PCR.

克隆的倍半萜合酶氨基酸序列与烟草马兜铃烯合酶、莨菪岩兰螺旋二烯合酶、棉花杜松烯合酶的一致性分别为39％，38％和41％；与青蒿柏木脑合酶、紫穗槐二烯合酶和一个推测的倍半萜合酶克隆cASC125的一致性为50％，48％和59％。cDNA编码区序列被克隆进原核表达载体pET-30a，并在大肠杆菌BL21（DE3）中诱导表达，但过量表达的蛋白主要是以不溶性蛋白形式存在。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。