查询词典 RT

Methods With β-actin gene as a reference, reverse transcriptase polymerase chain reaction was conducted to dectect the expression of PSA mRNA in 35 BC samples, 12 paracarcinoma breast tissues and 10 breast adenofibroma samples.

以β actin作为内部参照半定量RT PCR方法检测 35例乳腺癌组织、12癌旁乳腺组织和 10例乳腺纤维瘤中PSAmRNA的表达。

It indicated that in rape, six of ABCD genes that are Apetalal, Apetala2, Apetala3, Pistillata, Agamous and Agamous-like11, exhibited a variation in the expression level at the different development stages.

以油菜处于花蕾发育过程7个生长期的花蕾和花朵为材料，利用优化的半定量RT-PCR技术在转录水平对ABCD模型的6个功能基因，即Apetalal、Apetala2、Apetala3、Pistillata、Agamous、Agamous-likell，进行分析，探讨了油菜花蕾发育及开花过程中6个功能基因表达的差异。

We cloned the Rana temporaria chensinensis David skin cDNA using a PCR strategy and detected its activity in rana using a antibacterial protein assay against Micrococcus luteus. The cloning was based on a report of GenBank. The deduced amino acid sequence resulted in 53 amino with 46% identity to amphibia. RT-PCR was used to detect peptide mRNA in Rana temporaria chensinensis David.

我们用提取中国林蛙皮肽总RNA，逆转录合成cDNA，PCR扩增得到DNA片段，并与GenBank上登陆的其他蛙类的皮肽抗菌肽序列进行比较，发现此序列是一个新的蛙皮肽抗菌肽序列。

RT-PCR amplification of ginseng β-amyrin synthase gene was successfully performed based on the total RNAs extracted from ginseng hairy roots induced by Agrobacterium rhizogenes A4 using a modified guanidine isothiocyanate-method.

以发根农杆菌A4菌株诱导的人参发根为材料，用改良的异硫氰酸胍法提取总RNA，得到了纯度好的完整的总RNA。

Expression of a novel antra????poptosis gene, survivin, correlated with tumour cell apoptosis and p53 accumulation in gastric carcinomas［J］.

myc在癌旁正常食管黏膜组织中均未见表达，与多数文献报道一致［1， 7］。survivin为新发现的凋亡抑制基因，其在食管标本中的研究较少，SARELA等［8］及KATO等［9］均用RT？？？？

Maternal TGFαmRNA was gradually reduced from oocyte to 8-cell stage and maintaining lower TGFαmRNA level was to morula stage, assumably TGFαmRNA transcription was begun from 8-cell stage with the zygote genome activation.

EGF、TGFα和EGFR mRNA在生殖系统中的表达通过RT-PCR的方法检测了EGF、TGFα和EGFR mRNA在生殖系统中的表达。

METHODS: 40 rats were randomly divided into four groups:normal group, asthenic spleen group, high-dosage group and low-dosage group with 10 rats in each group, the asthenic spleen rat models were established in group B, C group as well as group D. Rats in group B, group C, group D were fed with normal distilled water, high-dosage of Guizhi decoction and low-dosage of Guizhi decoction respectively, NFAT mRNA, IL-4 mRNA, IFN-γmRNA were determined by PCR.

40只大鼠随机分为空白对照组、脾虚组、桂枝汤大剂量组和桂枝汤小剂量组等4组，每组10只；用规范的脾气虚证大鼠模型造模方法将B组、C组、D组大鼠造模，B组、C组、D组分别用蒸馏水、桂枝汤大剂量、桂枝汤小剂量灌胃，标本采集后用RT-PCR检测NFAT mRNA，IL-4 mRNA和IFN-γ mRNA表达水平。

METHODS: Gastric carcinoma cell line MKN45 at logarithmic growth phase was subcutaneously inoculated in 20 athymic mice.

将对数生长期的胃癌细胞株MKN45接种到裸鼠皮下，成瘤后用制备好的分别含GCRG213正、反义克隆和RNAi片段的腺相关病毒分别在肿瘤局部注射，以空病毒和生理盐水对照，继续喂养2 wk后处死，取肿瘤测量重量，并用半定量RT-PCR检测肿瘤组织GCRG213mRNA表达水平。

Methods］ Healthy SD rats were divided randomly into 6 group, including: normal control group(sham-operation), ischemia for 45 min (reperfusion 0h, n=6), reperfusion 2,4,12 and 24 hr group. Renal ischemia reperfusion injury models were founded in other five groups, opened abdominal and placed atraumatic vascular champ in dual kidney pedicle for 45 minutes,followed by reperfusion 2,4,12 and 24 hr.

方法］ 将SD大鼠随机分成正常对照组、单纯缺血组、再灌注2 h组、4 h组、12 h组、24 h组，制备双侧肾蒂夹闭模型后，腹主动脉取血测血肌酐、尿素氮，行肾脏组织病理学检查，免疫组化法检测TGFβ1 蛋白，RT-PCR法检测TGFβ1 mRNA。

To establish the base of the control of the Citrus aurantium L. fruit maturity in molecular level and produce high quality Fructus Aurantii, a pair of degenergte primers were designed according to the conserve regions of ACC Oxidase gene of many kinds of plants.

根据已报道的各种植物ACC氧化酶氨基酸序列上前后两个保守区设计1对简并引物，以酸橙叶片总RNA为模板，通过RT-PCR扩增到1个834bp的基因片段。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。