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In order to avoid prolin residue inhibiting enterokinase cleavage, 9bp of MGF cDNA 5′ end sequence was truncated by primer, then the obtained truncated MGF des(1-3MGF cDNA (321 bp) was subcloned in pET32a vector to construct a prokaryotic recombination expression plasmid.

通过RT-PCR从拉伸刺激的人成骨细胞中克隆MGF cDNA序列，并去除5'端9 bp的序列，使N端缺少对肠激酶(Enterokinase， EK)具有抑制作用的脯氨酸，将截短型MGF des(1-3 MGF cDNA序列克隆入pET32a质粒，构建重组表达质粒。

Results: The result is that PCNA positive appeared 6 hours after transplantation, increase unit time pass by, and reach peat at 1 to 2 week. After 2 weeks, PCNA positive of SMC in the tunica media begin to decline and recover to the level 6 hours after the operation at the 8 weeks.

本实验的第三部分，我们将于液氮固定的移植静脉段经组织匀浆后用于研究ECE和ET-1的表达情况，我们分别从mRNA水平和蛋白质水平，采用RT-PCR和免疫组织化学方法，对ECE和ET-1在移植静脉的表达情况进行分析。

The effects of BMP-2, TNF-α and they two synergism in different concentration were also detected. The mRNA expression of cbfal, OC, H-ras, BMPR-IA, type Ⅰ and typy Ⅱ collagen of ligamental fibroblast under the co-stimulation of BMP-2 and TNF-α were detected using RT-PCR technique.

并分析了不同浓度的人重组骨形态发生蛋白-2（rhBMP-2，10ng/ml、100ng/ml、250ng/ml、500ng/ml）和人重组肿瘤坏死因子-α（rhTNF-α，5u/ml、50u/ml、100u/ml、500u/ml）及两者协同作用（500ng/ml rhBMP-2+50u/ml rhTNF-α）对两类细胞系分化方向（细胞形态变化、ALP活性、DNA及胶原合成、骨钙素分泌）的作用。

Adenovirus 5第二军医大学博士学位论文英文摘要胸心外科专业(WAd5) and PDC-MLC-VEGF were transferred to the adenoviral packaging cell HEK 293cell by lipofectamine 2000 mediated gene transfer method to pack the virus; The desired Advectors were purified by density gradient ultracentrifuge and titrated in 293 cells afteridentified.

Ad.mlc-hVEGF165 体外转染心肌细胞的实验研究分离培养乳鼠心肌细胞，在同一 MOI 值下，Ad.mlc-hVEGF165 和 Ad.hVEGF165分别转染心肌细胞，以 RT-PCR 法和 Western 印迹分析法分别检测转染后心肌细胞内VEGF mRNA 的转录及 VEGF 蛋白的表达，以 ELISA 法检测转染后心肌细胞分泌的VEGF 蛋白质，MTT 等方法观察其对促内皮细胞增殖作用。

In this study, the full length cDNA, encoding Rubisco large subunit was cloned from Ulva clathrata for the first time.

首先应用RT-PCR方法获得了rbcL大片段cDNA序列，序列长度为1101bp ，并进行了测序分析。

In the first pa.rt,we clolinc the TC inodnhis of convexity and TC modulus of smoothness of quasi-Baiiach space, and prove that the space which is characterized by uniform convexity is same as the space which is cliaracteri/,ed by uniform TC convcxity.Then we give several characterizations of q-uniformly TC convex quasi-Banach space.At the same time ,we prove the triionned-theorcm.In the second part, we give the relationships between some inequalities of martingales with values in quasi-Banach space and uniformly TC convex quasi-Banach space.

本文分四部分，第一部分在拟Banach空间上定义了TC凸性模和TC光滑模并证明了在Banach空间上它分别和一致凸性和一致光滑性刻划的空间是一致的，即Banach空间X是一致TC凸的的充分必要条件是它是一致凸的，Banach空间X是一致TC光滑的充分必要条件是它是一致光滑的，还分别得出了判定一致TC凸和一致TC光滑的几个充分必要条件，同时还证明了在拟范数下的重赋范定理。

Suppose that a region was scanned uninterruptedly by three satellites in different phases.

在充分构思各种数据结构的基础上，给出了基于RT-Actor模型的Real-Time Java目标代码。

Furthermore, the experimental k values were used to determine the associated activation energy, Ea, for the formation of La2/3TiO3-type phase using the Arrhenius plot and the following least-square equation, ln =-Ea/RT + A, where T is the annealing temperature, R is the universal gas constant, and A is a constant, resulting in Ea ~ 607 ?

第二部分则是以微观的方式来解析Pb2+离子与La4Ti9O24陶瓷以及La2/3TiO3-type相晶格之间的关系；利用化学共沉法来合成不同Pb2+离子添加量的La4Ti9O24陶瓷起始粉体，再以900 oC /1h的条件热处理；藉由选区电子绕射图谱以及X-ray绕射分析所生成之La2/3TiO3-type相结构；结果显示La2/3TiO3-type相之结晶结构为斜方晶之钙钛矿结构，符合空间群IbmmNo。

Methods A total of 60 adult female SD rats weighing about 340g were randomly allocated into 3 equal groups: unoperated control group (n=20), sham-operated group (n=20) and ovariectomied group (n=20). Immunohistochemical methods were used to determine ER-αand ER-βin superficial and deep compartments in the uterus and vagina.

60只成年SD雌性大鼠随机分为正常组、假手术组、去势组，每组20只。8周后处死大鼠，分离摘取子宫、阴道，进行ERα、ERβ免疫组织化学染色和mRNA半定量RT-PCR检测。

One unspecific siRNA fragment was also synthesized as the negative control.

向Hela细胞中转染重组载体，RT-PCR证明在Hela细胞中成功表达Nanog基因。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。