查询词典 RT

A 3-1E gene fragment was amplified from Eimeria acervulina SH strain sporozoite by RT-PCR,and it was cloned into eukaryotic expression vector pcDNA3.1 to construct the recombinant plasmid pcDNA3.1-3-1E.

应用反转录-聚合酶链式反应技术从堆形艾美球虫SH株子孢子中扩增3-1E基因片段，将其克隆入真核表达载体pcDNA3.1中，构建重组表达质粒pcDNA3.1-3-1E。

The result of RT-PCR showed that MuMADS1 just expressed in the flower and fruit but not in the root、 stem and leaf.

根据其表达量的变化，推测MuMADS1可能除了花的发育起作用外，在果实发育的早期与果实的发育相关，而在果实发育的晚期与成熟相关。

Methods: 2 hours of reversible focal ischemia in rats were produced by improved the method of Connolly' suture occlusion of MCA and the expression of SAMDC-mRNA in the cortex and subcortex was measured by RT-PCR after 2,4,8 and 24h reperfusion following 2h ischemia respectively.

在Connolly线栓法的基础上进行改良，复制大鼠MCA区2h脑缺血-再灌流2，4，8，24h动物模型，用逆转录多聚酶链反应测定缺血区皮质和皮质下组织不同时相SAMDC-mRNA的表达。

In this study,the full length cDNA of nAChR α subunit from this pest was cloned by RT-PCR.

序列分析表明，这是 1个新的α亚基基因，定名为Csα 1。

Results: Mdroxyprogesterone acetate plus E2 (10-8 M) stimulated both the mRNA levels and activities of sulfatase (at 10-8-10-6 M) compared to E2 only.

用同位素标记的底物和RT-PCR技术分别测定硫酸酯酶和硫转移酶的活性和mRNA表达水平。

The cell supernatant was collected after 3 and 6 days.

RT-PCR检测细胞Ⅱ型胶原和aggrecan的mRNA表达水平。

The levels of plasma and myocardium of malondialdehyde, superoxide dismutase and cAMP were measured.

原位杂交及RT-PCR观察ADM mRNA在各组心肌表达的变化。

The frequency of losing exon 5-8 was the highest . It was consistent with the results of the FHIT gene whole length cDAN by RT-PCR.Conclusions: The above results showed that the changes of FHIT gene is probably related to the development of kinds of tumors, which is an important molecular event. FHIT gene can be considered to be a tumor suppresser gene, our studys provided a theoretic and experimental data for further research on the function of FHIT gene.

结论本研究结果初步证明FH工T基因改变可能与多种肿瘤的发生有关，是肿瘤发生的一个重要的早期分子事件，支持其作为一个抑癌基因在肿瘤发生中发挥作用，为进一步深入研究FH IT基因的功能提供了理论和实验依据。

Materials and Methods : From January 1996 to December 2003, 92 lung cancer patients with SVC syndrome were referred for RT.

材料与方法：从 1996 年 1 月到 2003 年 12 月，我们接受了 92 位原发性肺癌合併上腔静脉症候群的病患的会诊而进行放射治疗。

AIM: To isolate bioactive secondary metabolites from the fruits of Schisandra rebriflora Rehd. et. Wils and study their effects on HIV-1 infectivity. METHODS: Inhibition of syncytia formation, HIV-1 reverse transcriptase and protease, protection of HIV-1 infected cells, level of HIV-1 p24 antigen,blockage of fusion were detected. RESULTS: A lignan compound, named rubrifloralignan A, was first isolated as a natural product with modest inhibition effects on syncytium formation and HIV- 1 replication and could protect HIV-1 infected cells with weak inhibition effects on fusion and RT, but it showed no effects on the replication of HIV-1 chronic H9 and PR. CONCLUSION: Rubrifloralignan A is an efficient anti-HIV-1 product with effect on early stage of HIV-1 replication.

目的：从红花五味子果实中寻找活性代谢产物，研究其抗HIV—1活性和作用机制方法：通过合胞体抑制、HIV-1感染细胞保护、HIV—1p24抗原测定、融合阻断、逆转录酶和蛋白酶活性分析等实验，检测分离化合物的抗HIV—1活性并探讨其作用机制一结果：红花五味子甲素能够抑制病毒诱导的合胞体形成，保护病毒感染细胞，抑制病毒在细胞内的复制对HIV-1感染细胞与正常CD4^＋细胞间的融合，逆转录酶活性有一定的抑制作用但是红花五味子甲素不抑制病毒在慢性感染细胞中的复制，也不抑制蛋白酶的活性、结论：首次从天然产物中分离得到红花五味子甲素，它对HIV—1病毒复制早期具有抑制作用

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。