查询词典 RT

To produce the scientific evidence for developing and manufacturing new antitumor drugs.Methods: 1 The inhibitory effect on cell growth of Hela was measured by MTT assay in treated or untreated groups (3.125, 6.25, 12.5, 25, 50μg/ml TAM and control) for three different treatment times (24h, 48h and 72h).2 Apoptosis and cell cycle were measured by FCM in four experimental groups (0, 4, 16, 40μg/ml TAM) for 48h.3 Adopting Wright and Giemse's staining to observe the morphology of Hela cells which treated with 40μg/ml TAM.4 Using invasion experiment to detect the Hela cells'invasive abilities which treated with 40μg/ml TAM.5 The protein expressional levels of P-ERK, ERK, C-myc and Cyclin D1 in Hela cells untreated or treated with 4, 16, 40μg/ml TAM for 24h were measured by Western blot.6 Expression of anti-apoptotic gene bcl-2, apoptotic gene bax and MMP-9 in Hela cells of four experimental groups (0, 4, 16, 40μg/ml TAM for 24h), were observed by revers transcription PCR.7 The protein expression of P-ERK, ERK, Bcl-2 and Bax in Hela cells treated with 40μg/ml TAM for 24h observed by laser scanning microscopes.

1采用四甲基偶氮唑蓝法检测不同浓度北豆根总碱(3.125、6.25、12.5、25、50μg/ml)处理不同时间(24、48和72小时)对Hela细胞增殖反应的抑制作用。2采用流式细胞技术(flow cytometry，FCM)检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用48小时，对Hela细胞凋亡及周期变化的影响。3瑞氏-姬姆萨染色后显微镜观察北豆根总碱(0、40μg/ml)作用24小时后Hela细胞形态学变化。4采用Transwell小室法检测北豆根总碱(0、40μg/ml)作用24小时后对Hela细胞侵袭性的影响。5采用免疫印迹方法检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用24小时后，Hela细胞中磷酸化ERK、ERK、C-myc、CyclinD1的表达变化。6采用逆转录-聚合酶链反应(revers transcription PCR，RT-PCR)半定量检测北豆根总碱(0、4、16、40μg/ml)作用24小时，Hela细胞抗凋亡基因bcl-2、促凋亡基因bax、基质金属蛋白酶-9(MMP-9)的表达变化。7应用激光共聚焦显微镜(laser scanning microscope，LSM)观察北豆根总碱(0、40μg/ml)作用24小时后，Hela细胞内P-ERK、ERK、Bcl-2、Bax蛋白的表达变化。

This method is efficient to detect SFV in rhesus monkey, and it has great significance for quality control of laboratory monkeys.

所建立的SFV RT-nestedPCR检测方法能准确的检测出恒河猴SFV的感染情况，对控制实验猴群的质量具有重要意义。

The deduced amino acid sequences of HbHDT1 showed high identities (65％, 59％, 50%, 45%, 43％ and 40%, respectively)to those of the histone deacetylase from Ricinus communis, Populus trichocarpa, Solanum chacoense, Trifolium pretense, Glycine max and Triticum aestivum.

该氨基酸序列与蓖麻、毛果杨、马铃薯、红花苜蓿、大豆、小麦的HDACs家族成员的同源性分别为65％、59％、50％、45％、43％和40％。半定量RT-PCR分析结果表明HbHDT1在巴西橡胶树的花、愈伤组织、胚、叶、芽、胶乳中均有表达。

The expressions of RhoA, ROCK1/2 and COL Ⅱ in the induced BMSCs and after application of enzyme inhibitor Y27632 were detected by Western-blot and BT-PCR.

采用Western-blot和RT-PCR方法分别检测RhoA和ROCK1/2Ⅱ型胶原在细胞分化过程中的表达及应用Rho激酶抑制剂Y276532后的表达。

Methods: The experimental g roup consisted of 24 patients with PE and 24 cases of normal placentae women respectively. Group of 24 patients with PE consisted of 12 mild cases and 12 severe cases. RT-PCR and Western Blotting were used to test the difference content of HLA-G between two groups.

采用半定量逆转录-聚合酶链技术及Western印迹技术分别检测了24例子痫前期患者(实验组，轻度子痫前期12例，重度子痫前期12例)，24例年龄相仿的正常晚期妊娠者胎盘组织中HLA-G的表达差异。

RT I love you not because of who you are, but because of who I am when I am with you.

我爱你，不是因为你是一个怎样的人，而是因为我喜欢与你在一起时的感觉。

Rt of life, something we're all destined to do.

死亡是生命的一部分，是我们注定要做的一件事。

I know that i 'm just d i rt !

我知道我仅仅是污垢！

Then, she fell in love with this a rt.

然后，她爱上了这一艺术。

It is not because you are beautiful and sma rt and perfect.

这并不是因为你漂亮聪明或者完美。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。