查询词典 RT

The markers of hepatic linear cells were examined before induction ,and 7, 14, 21 and 28 day after induction by RT-PCR, immunocyte chemistry and PAS (periodic acid-schiff) in every group.

人脐带血单个核细胞、间充质千细胞均可在体外诱导分化为具有肝系细胞表型和功能的细胞。4。

We detected the PERV DNA and RNA by PCR and RT-PCR.

检测混合型人工肝支持系统术前病人的血清均呈阴性。

The wound surfaces in the experimental group were treated by arnebia root oil and those in control group were treated by petrolatum gauze.

用组织学、组织化学、电镜等方法观察创面的组织结构和愈合率、用Western-blot，RT-PCR方法观察2组的bFGF及其mRNA表达。

Technical parameters for the separation and purification of phenazine from the waste and synthesis of phenazine methyl sulfate were studied.

研究了RT培司废料处理及提纯吩嗪的方法，以及吩嗪硫酸甲酯合成的优化工艺条件。

Expression of PS2 and ITF was determined with RT-PCR,and the determination of contents of aninohexose and phosphatide were carried out.

逆转录-多聚酶链反应检测乳癌相关肽（PS2）、肠三叶因子基因表达变化，并同时检测氨基己糖及磷脂含量。

The expression level of integrin β1 on the surface of K562 cells was evaluated by flow cytometry; the growth of K562 cells treated with phycocyanin was measured by MTT assay; the expression level of FAK mRNA was analyzed by relatively quantitative RT-PCR after four-day culture of K562 cells with phycocyanin of 40 μg/ml,80 μg/ml and 160 μg/ml,respectively.

本 研究观察不同浓度藻蓝蛋白对K562细胞增殖的影响，并检测藻蓝蛋白处理后细胞表面整合蛋白β1表达和胞内黏着斑激酶基因表达的变化，探讨整合蛋白β1在藻蓝蛋白抑制K562细胞增殖中的可能作用机制。

Total RNA was isolated from cultured human pigmental cells and mRNA was reversly transcribed into cDNA. Then PCR was used to amplify the TRP -2 coding region. The PCR product was cloned into pUC19 plasmid and sequenced, then subcloned into vector pGEX-4T-1. The TRP-2 protein was expressed hi E coli of DH5 a as fusion protein with glutathioneS-transferase induced by IPTG. The fusion protein was purified by glutathione resin column.

我们通过RT—PCR的方法扩增TRP—2编码基因，克隆至pUC19载体并测序，再亚克隆至GST融合表达载体pGEX—4T—1，转化大肠杆菌，IPTG诱导表达了GST/TRP—2融合蛋白，并分析鉴定表达蛋白的可溶性，最后第四军医大学硕士学位论文一用谷氨酚胺树脂柱纯化表达的蛋白。

The study mainly consists of two parts: the establishment of an RT-framework for drama interpretation and its application to the analysis of Harold Pinter's plays.

这项研究主要包括两部分，其一为从关联理论角度建构戏剧阐释的框架，其二是将这一理论框架用于对哈罗德·品特剧本的分析。

Results of the RT-PCR showed that the increased transcription of T6 is correlated with the infection by plasmodium parasites.

对与先天免疫相关的分子进行的半定量实验表明，大劣按蚊T6基因的转录与感染疟原虫相关。

Transgenic tobacco plants were obtained through screening with kanamycin. The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants. Pokeweed antiviral protein Ⅱ is expressed with high level in summer leaves. The expression of PAPⅡ is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPⅡ gene by RT-PCR and then the gene was cloned into E. coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPⅡ gene were then transferred into E. coli strain BL21 (DE3)-plysS and Pachia pastoris GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陆抗病毒蛋白Ⅱ是一个受季节调控表达的蛋白，本实验以美洲商陆夏季叶片为材料，通过对其总RNA的提取、反转录、并用PAPⅡ的特异引物进行PCR扩增，对PAPⅡ进行了基因克隆、序列分析，结果扩增出来的PAPⅡ基因与已经报道的序列同源性是99.9％，然后将该基因构建到原核、真核表达载体上，分别转化了大肠杆菌和毕赤酵母并对它们进行了诱导表达，在两个表达系统中均获得了有活性的PAPⅡ表达蛋白，体外生物测定表明表达的蛋白均具有抑制病毒的活性，PAPⅡ基因在酵母中还没有表达的报道，这为获得具活性PAPⅡ蛋白提供了一种简便可行的方法。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。