查询词典 RT

The aim of the study is to understand the effect of Tobacco etch virus on maize dwarf mosaic disease. In the net house, an experiment is carried out by means of artificial inoculation.

为了研究烟草蚀纹病毒(Tobacco etch virus， TEV)对玉米矮花叶病的预防效果，在防虫网室内，采用人工摩擦接种病毒的方法进行生物学试验，并用RT-PCR和实时荧光定量PCR技术对预防效果进行了检测。

Moschus berezovskii IL-2 was cloned from Con-A stimulated peripheral blood mononuclear cells, and ligated with pIVID18-T vector, then transformed to Escherichia coli JM109. The positive recombinant was sequenced.

用林麝外周血单核细胞(peripheral blood mononuclear cells， PBMC)提取总RNA，RT-PCR扩增IL-2(interleukin-2， IL-2)，连接pMD18-T载体后转化大肠杆菌，筛选阳性克隆并测序。

To explore the function of PDE in canine central nerve , gene expression of 18 PDE subtypes in different fractions of canine central nerve (cerebrum,cerebellum,myelencephalon and spinal cord) were detected by RT-PCR,and PDE activity was calculated by the content changes of cyclic nucleotides before and after the PDE reaction examined by high performance liquid chromatography.

为探明磷酸二酯酶(phosphodiesterase，PDE)在犬中枢神经活动中的作用，采用反转录聚合酶链反应检测18个PDE亚型在犬中枢神经不同部位中(大脑、小脑、延髓和脊髓各段)的表达分布，以高效液相色谱法检测环核苷酸在酶反应前后的含量变化，计算PDE活性。

Methods:Control trial was adopted in the experiment design.Immunochemistry and RT-PCR were used to detect the expression of MMP-7 in nasopharyngeal carcinoma and chronic nasopharyngitis.

采用成组设计的对照试验方法，运用免疫组化及逆转录聚合酶链式反应技术，检测MMP-7蛋白及mRNA在鼻咽癌及慢性鼻咽炎组织中的表达。

Fc-vasa mRNAs were detected by RT-PCR in early developmental stages from 2-cells embry to intramembranous nauplius, and not in any stages after nauplius. Based on above results, it is postulated that Fc-vasa is required for germ cell determination and formation of F. chinensis as a maternal component.

但表达量逐渐降低，自出膜后的无节幼体开始检测不到表达信号，根据以上结果推测，Fc-vasa mRNA可能作为母源生殖质成分参与中国明对虾生殖细胞的决定和形成。

PACAP/GHRH mRNA was detected in grouper early embryos but was strongly expressed starting on neurula stage onwards.

在此基础上，利用半定量RT-PCR方法对斜带石斑鱼胚胎及仔鱼期的GHRH／PACAP前体mRNA的表达情况进行了检测。

By RT-PCR and in situ hybridization, we found Pmr1 was expresses from early neurula (st.15) in the ventral part of embryo, then located in the anterior ventral blood island which is the primitive myeloid cells generated region.

Pmr1是从非洲爪蟾肝组织提取出的mRNA核酸内切酶，参与雌激素诱导的mRNA降解过程。但是Pmr1在胚胎发育中的分布和作用尚不明了。

Nighty rats were randomly divided into the groups of control, myocardial infarction and doxycycline, which were given orally 48 hours before and 48 hours after MI with 30mg/Kg per day. Protein and mRNA extraction was done on left ventricular samples containing scar and myocardium together. Samples were assayed from 1 day to 4 weeks post-Mi. The activity of MMPs was measured by zymography and collagen amount by the method of chloramine T, the ratio of II III collagen was assessed by immunohistochemical stain. Protein and mRNA of MMP-2, 9, TIMP-1 were determined by Western-Blot and RT-PCR.

分为对照组、心梗组和治疗组(D组，术前两天至术后两天，口服Doxycycline，30mg／kg／天)，分别于术后第一天、术后一周、术后两周、术后四周取心肌组织，采用免疫组化测定胶原含量和Ⅰ／Ⅲ胶原比例，酶谱法测定心梗后MMP-2，9活性蛋白的表达规律，Western Blotting进一步确定酶谱法中所消化条带蛋白的属性，逆转录-聚合链反应法测定心梗后MMP-2，9和TIMP-1的mRNA的变化规律，运用Acuson Sequoia 512超声心动图机分别测定心梗组、治疗组在两周和四周时左室前壁、后壁厚度以及左心室舒张末内径和左室射血分数。

Methods: In order to study P〓、P〓 and bcl-2 gene expression and its significance in tumorgenesis of lung cancer, the expression of P〓、P〓 and bcl-2 gene was detected by RT-PCR, PCR-SSCP and immunhistochemistry SABC in 65 cases of lung cancer tissues, tissues adjacent to carcinoma and normal tissues, its correlation to clinico-pathological features and prognosis of lung cancer was explored.

因此人们预测其重要性不亚于RB和P〓基因，甚至比其更为重要。Bcl-2基因是继促进细胞生长的癌基因和突变的抑癌基因之后，近年来发现的第三类肿瘤基因，它是一种调控细胞死亡的"存活基因"，可抑制细胞的程序化死亡，故称之为细胞凋亡抑制基因，在肿瘤发生、发展中起着重要的作用。

To explore the functions of SPANXA1 in cancerous phenotypes CL1-5 cells were transfected with a SPANXA1 expressing vector and evaluated by cell proliferation, cell migration, Matrigel invasion and colony formation assays in vitro as well as mouse metastasis assays in vivo. The results indicated that the induction of SPANXA1 could reduce cell invasiveness. In the other hand, immunostaining showed that SPANXA1 was predominately located at the nucleoplasm.

经RT-PCR验证得知SPANXA1在CL1-0的表现量比CL1-5多100倍以上，於是我们将SPANXA1转染在CL1-5细胞株中，并测量活体外的细胞生长速度试验、细胞迁徙实验、Matrigel侵袭实验、癌细胞群落形成实验及小鼠活体内细胞转移能力，探讨SPANXA1对癌细胞性状的影响，结果发现增加SPANXA1会降低癌细胞的转移能力，在另一方面，我们利用免疫萤光染色法侦测出SPANXA1是存在於核质中，并以西方点墨法再次证实SPANXA1是存在於核质中。

Finally, according to market conditions and market products this article paper analyzes the trends in the development of camera technology, and designs a color night vision camera.

最后根据市场情况和市面上产品的情况分析了摄像机技术的发展趋势，并设计了一款彩色夜视摄像机。

Only person height weeds and the fierce looks stone idles were there.

只有半人深的荒草和龇牙咧嘴的神像。