查询词典 RT

Methods Rat models of corneal isograft and corneal allograft were established, The submandibular lymph nodes were harvested before operation and at day 1, 3, 5, 7, 10, 14 after operation. The expression of CCL19 mRNA in draining lymph nodes were detected by reverse transcription- polymerase chain reation, and their chemotaxis to dendritic cells were examined in Boyden chamber.

建立大鼠正位穿透角膜移植模型，RT-PCR法测定正常角膜0d及角膜移植后1d，3d，5d，7d，10d，14d的局部淋巴结内趋化因子CCL19 mRNA的农达变化，应用Boyden小室进行不同时相淋巴结组织液对体外培养树突状细胞的趋化实验。

In the present study, three Y-box cDNAs, named as Lyb1, Lyb2, and Lyb3, were cloned from the liver and oral gland of Japanese lamprey using reverse transcript-PCR and rapid amplification of cDNA ends techniques.

采用RT-PCR和RACE技术，从日本七鳃鳗口腔腺、肝脏中分离获得了3条Y-box基因序列，分别命名为 Lyb1、Lyb2和 Lyb3，生物信息学分析其编码的蛋白质序列长度分别为331、181和171个氨基酸残基。

The relative expression in Silky eye is more than two times that of white Leghorn.

RT-PCR结果显示，鸡Slc24a5基因在多处组织表达（眼、脑、皮、肉、心、肝、肾和肺）。

In our study,two important lepidopteron pests,beet armyworm and cotton bollworm were surveyed for their endogenous piggyBac.As a result,endogenous piggyBac elements were found in these two insects,and one of the piggyBac elements isolated from the genome of cotton bollworm was intact and thought to be potentially active.1 Clonging and sequence analysis of piggyBac from beet armywormUsing PCR technique,with degenerate primers,a DNA fragment of piggyBac-like element was cloned from the genome of beet armyworm,spodoptera exigua hubner.The DNA fragment was 456bp in length,and the deduced amino acid sequence shares 50%- 78% similarity with other piggyBac elements from insects.But one stop code was found in this DNA fragment.

由于转座子很难用常规的RT-PCR和RACE技术进行克隆鉴定，故本研究在兼并引物PCR的基础上，利用inverse PCR和vectorret PCR技术，不仅调查了鳞翅目两种重要的农业害虫甜菜夜蛾和棉铃虫的內源piggyBac存在情况，同时克隆到了內源性的piggyBac转座子，并且从棉铃虫中获得了一个结构完整、具有潜在活性的piggyBac转座子HaPLE1.1甜菜夜蛾piggyBac转座子基因的克隆与序列分析采用PCR技术，利用兼并引物，从甜菜夜蛾基因组中克隆出一个內源性piggyBac类似因子的DNA片段，并命名为SePLE。

SSEA-1and Oct-4 were not detected at the protein nor the mRNA level in the limbus and cornea.

免疫组织化学法和RT-PCR均显示SSEA-1和Oct-4不表达与角膜缘上皮和角膜上皮。

Results showed that the standard curve made by pMD-18T-GHR had good linear dependence, and the fluorescent quantitative RT-PCR assay was sensitive, specific and reliable.

结果表明，由pMD-18T-GHR所构建的标准曲线线性关系良好，建立的GHR基因荧光定量PCR检测方法灵敏度高、特异性强（可以检测出低于10个拷贝/μl的样品），准确可靠。

There was obvious linear function relationship between the rotation angles of stimulus pictures and RT.

刺激图片的旋转角度与反应时之间存在着明显的线性函数关系。

The sonic log curve is reconstructed by using BP neural network technology combining SP,Rt,Gr curves data as to enhance the information quality of curve reconstruction and ensure the accuracy of curve reconstruction.

运用BP神经网络技术综合了自然电位、电阻率、自然伽马等多条曲线的信息对声波曲线进行重构，以此增加曲线重构的信息量，为曲线重构的准确性提供了保障。

Its cDNA had been cloned by RT-PCR. The results of comparison on sequence homology suggested that Luffin P1 may be derived from a vicilin like precursor which is a storage protein in the seeds of Luffa cylindrica.

本文利用3'RACE法克隆了它的cDNA，并构建了它的融合表达质粒pGEX-4T-S2，该质粒在大肠杆菌中获得了成功表达。

Based on detailed working process of UWB-RI MAC, FI frame broadcast interval was analyzed and the support for QoS was explained. Finally, the performance simulation and comparison of UWB-RT MAC and UWB MACAW MAC were given.

通过给出的UWB-RI MAC协议数据收发的详细流程，分析了FI帧广播间隔的取值，说明其对QoS的支持；最后仿真比较了UWB-RI MAC和UWB-RI MAC和UWB MACAW MAC的性能。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。