查询词典 RT

Polymerase Chain Reactionwas performed using P1and P2as primers andthese eyestalk cDNAs as templates.Four specific cDNAs were cloned under properreaction conditions.

用无DNA污染的眼柄总RNA为模板，根据日本对虾的MIH设计兼并引物P1和P2，进行RT-PCR扩增，在适宜的反应条件下，均分别得到一特异性的产物。

Just before the mass began hundreds of doves were released at the end of a two-hour Concert of Hope that enlisted the participation of such major entertainment figures as tenor Ronan Tynan, Jose Feliciano, opera star Marcello Giord and Harry Connick Junior.

在弥撒开始不久将会有上百的各自在两个小时的希望音乐会尾声被放生，这个音乐会主要的参加者包括男高音歌唱家RT，JF，歌剧明星MG和HCJ。

So we consider the source of RT ferromagnetism of pure ZnO nanowires is related to the Zn vacancies in ZnO crystal lattice.

我们认为这与纯ZnO纳米线中的室温铁磁性来源与晶体的Zn空穴有关。

The effects of treatment were monitored by arthritis index,footpad thickness,microvessels density as well as the expression of vascular endothelial growth factor protein and mRNA in the synovium.

检测关节炎指数积分和炎症足垫厚度，免疫组化计算滑膜微血管密度，W estern B lot和RT-PCR方法分别检测滑膜血管内皮生长因子蛋白和mRNA的表达。

Four vectors of pET vector system were chosen to express the enzyme in E. coli BL21(DE3). The main results were as follows: 1. The total RNA was obtained from fungus Syncephalastrum racemosum.

以总状共头霉菌株总RNA为模板，通过RT-PCR方法，获得了去除自身信号肽的天冬氨酸类蛋白酶syncephapepsin基因。2。

The fresh fruit are distributed to RT-mart by supermarket department in the northeast,. The quality of fruitage and the assurance of come-in time has established the firm relationship with custom.

超市部主要为东北地区大润发超市配送新鲜水果，果品质量和时间的保证已经确立了稳定的客户资源。

The data suggested that Fc-vasa could be involved in gametogenesis of F. chinensis.

胚胎和幼虫RT-PCR分析检测到2细胞期至膜内无节幼的各期胚胎都含有Fc-vasa mRNA。

Result: 1.(1) Three strips come to appear in the position of 6.0kb, 5.4kb and 600bp after gelose electrophoresis and their size accord with recombined plasmid DNA, pcDNA3 plasmid and VEGF165 gene accordingly.(2) Pure degree of the pcDNA3-VEGF165 recombined plasmid is: OD260/OD280=1.87. Its

在进行了重组质粒的扩增、提取、纯化、鉴定、兔MSCs的原代及传代培养以及RT-PCR、Western Blot 转染检测后，实验取得了令人满意的结果，证实了MSCs 能在体外有效转录外源VEGF 基因并分泌出VEGF 蛋白，同时也说明pcDNA3-VEGF165重组真核表达质粒是有效的。

Second, low glutelin content was regulated at the level of transcription, but the suppressed glutelin genes were not among those published.

采用定量RT-PCR方法对LGC-1及其野生型日本优中已知谷蛋白基因逐个进行表达分析，结果表明，GluA-1，GluA-2，GluA-3和GluB-1，GluB-2，GluB-4基因的转录水平在二个品种中无明显差异。

The rabbits' RNA was extracted from the lavage cells, and mRNA for TNFα was quantitated by reverse-transcription polymerase chain reaction using glyceraldehyde 3-phosphate dehydrogenase as an internal standard.

第二部分：12只表面活性物质缺乏兔，以CMV(n＝6)或PHY+PEEPi(n＝6)通气1h后，重复灌洗，提取灌洗液中的兔RNA，以GAPDH为内参照RT-PCR的方法来定量分析TNFαmRNA。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。