查询词典 RT

Why RT can be relied upon for a study of cross-cultural pragmatic failure is one of emphases of my dissertation.

关联理论的框架是否可以用来支撑跨文化语用失误的研究是本文讨论的一个重点。

Results Administration of L-NAME could attenuate the activity of NOS and inhibit spermatogenic cells apoptosis in rats experimental cryptorchidism, and it could significantly inhibit weight loss of the cryptorchid.

手术建立大鼠单侧隐睾模型，实验分为隐睾组、隐睾+L-NAME组［术后腹腔注射溶于生理盐水的L-NAME 50 mg/(kg\5d)］、隐睾+生理盐水组、假手术组，7 d后采用化学比色法检测睾丸组织NOS活性，流式细胞术和原位缺口末端标记法检测生精细胞凋亡，RT-PCR和免疫组织化学法检测Bax基因表达变化。

The coat protein gene of Cucumber mosaic virusfrom melon isolate (CH99/90) was amplified by RT-PCR from the total RNA of infected zucchini leaves and cloned into pUCm-T vector.

把该分离物接种西葫芦，从发病的叶片中提取总 RNA，并以此为模板经 RTPCR 扩增获得 CMV 的外壳蛋白基因，将其克隆到 pUCmT 质粒上。

The growth curve of cellular clone was determined by trypan blue exclusion test.

RT-PCR和Western Blotting检测PNP-TK基因在HepG2细胞中的表达。

RT-PCR and immunofluorescence cytochemistry analysis was used to assess the differentiation.

结果：经诱导后，成肌细胞在形态上类似于神经元和星形胶质细胞。

Methods: The Hela cells were cultured in vitro and treated with different concentrations(0.125～2.0 mg/ml) of matrine solution for 24～72 hours. MTT assay was performed to evaluate the cytotoxin effect of matrine on Hela cells. FCM was used to detect the apoptosis of Hela cells. Transmission Electron Microscope analyses were carried out to examine the cell morphology.

选用人宫颈癌Hela细胞进行体外培养，以0.125～2.0 mg/ml的苦参碱分别处理Hela细胞24～72 h后，MTT法检测细胞增殖，流式细胞仪检测细胞凋亡率，透射电镜进行形态学观察，RT-PCR半定量检测凋亡相关基因Bcl-2和Bax的mRNA表达水平。

The main regions (405-1 146 nt) in the glyco pro tein gene of 8202,BRV and MRV,isolated from deer,cattle and mouse in China re spectively,of rabies virus was amplified by reverse transcriptase-polymerase ch ain reaction,cloned and sequenced.

对来源于鹿、牛和鼠的狂犬病病毒野毒株82 02、BRV、MRV的G基因405～1 146位核苷酸序列进行了RT-PCR扩增、克隆和序列测定，并应用计算机软件对这3个毒株的测定序列和人源株CGX89的相应序列进行了分析比较。

Results (1) The total RNA, reverse transcription product and fluorescence labeled cRNA were all of high quality;(2) There were 420 up-regulated genes and 552 down-regulated genes among 2-fold DEGs, including DKK1 (dickkopf homolog 1) which was 5-fold up-regulated;(3) The results of real time RT-PCR, using β-actin as an internal control, showed that the 2-ΔCt values of DKK1 in HCC, PCT and NLT were 0.089 504, 0.007 65 and 0.000 631 respectively.

结果 （1）配对组织总RNA质量高，反转录cRNA及荧光标记质量好；（2）2倍差异表达基因中，上调基因共420个，下调基因共552个，其中包括5倍上调基因DKK1；（3）以β-肌动蛋白为内对照的实时逆转录聚合酶链反应结果提示，DKK1在癌、癌旁和正常肝组织中的2-ΔCt值分别为0.089504、0.00765和

The reverse transcription polymerase chain reaction is a routine diagnostic assay for the detection of hMPV.

RT-PCR技术是hMPV主要的检测手段，hMPV感染也是哮喘急性发作和慢性阻塞性肺病急性加重的诱因。

Methods We measured messenger RNA levels of Dicer and Drosha in specimens of invasive epithelial ovarian cancer from 111 patients, using a quantitative reverse-transcriptase–polymerase-chain-reaction assay, and compared the results with clinical outcomes.

我们用定量RT-PCR的方法检测了111例侵袭性上皮卵巢癌标本中Dicer 和Drosha酶的mRNA的水平，并且分析其与临床预后的关系。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。