查询词典 RT

The bPRL mammary gland expression vectors were transfected into COS-7 (African green monkey kidney cell) and HC-11 (epithelial cell of mouse mammary gland) cells mediated with lipofectin, respectively. The transfected cells were cultured and harvested. RT-PCR analysis showed that bPRL mRNA was present in both COS-7 and HC-11 cells.

将构建的乳腺特异的表达载体，分别转染COS-7细胞株和HC-11细胞株，收集转染培养后的COS-7和HC-11细胞，通过RT-PCR实验，证实转染的细胞中在mRNA水平均获得了表达。

In order to confirm this suspect, we choosed a cultured rat granulation tissue fibroblasts model in vitro and applied RT-PCR and cell-ELISA technology to detect the changes of firbroblasts intrinsic EGF, bFGF, TGFβ-1 with their receptors gene expressions and protein synthesises after stimulated by SP. Our purpose was to explore the possible effects and patterns imposed by SP on fibroblasts intrinsic growth factors and their receptors expressions, which maybe offer theoretical basis for promoting wound healing via improving nervous functions and regulating neuropeptides secretions.

为进一步验证这一推测，同时排除在体多因素干扰，我们采用了一种大鼠肉芽组织成纤维细胞体外培养模型，采用RT-PCR与细胞ELISA技术，检测SP刺激成纤维细胞后，成纤维细胞内源性EGF、bFGF、TGFβ-1及其受体基因表达和蛋白合成的改变情况，探讨SP对成纤维细胞内源性生长因子及其受体表达的影响以及方式，以期为经由改善神经功能、调节神经肽分泌途径促进伤口愈合提供理论依据。

Due to specificity on enterovirus,the RT-PCR method has some value in the diagnosis of enterovirus infections.

该RT－PCR方法具有肠道病毒特异性，在肠道病毒感染的诊断中有一定应用价值。

The transcriptions of OsGS in rice under normal and stress conditions were studied by RT-PCR method.

通过RT—PCR对OsGS在水稻正常生长条件和逆境条件下的表达进行了研究。

During hypoxic exposure,the leakage of LDH was gradually increased , peaked at 8h ,then decreased. The same results were supported by electron telescope. At the same time, we used RT-PCR to observe the expression of HIF-1 a rnRNA.

在检测细胞损伤的同时，我们采用RT-PCR技术观察缺氧后HIF-1α mRNA的表达，发现在缺氧0h也有低表达，而后表现为时间依赖性，在缺氧4hmRNA的表达达到高峰，而后逐渐降低。

R20- revise to absolute value of 20℃, Rt- is the absolute resistance vale under measuring temperature

式中：R20-校正到20℃时的绝缘电阻值；Rt-在测量温度下的绝缘电阻值

More sensitive RT-PCR was used to monitor tumor load when it was lower to bcr/abl by FISH during treatment.

FISH检测bcr/abl转阴的病人需靠灵敏度更高的RT-PCR监测。

The main functional region of the F gene including 535 nucleotides was amplified by RT-PCR and sequenced.

运用RT—PCR扩增了该分离株F基因的重要功能片段（约535kb），并进行了序列测定。

The fragment of F gene covering cleavage site region was amplified by RT-PCR, cloned and compared. Basing on published sequences of representative NDV strains, a phylogenetic tree was constructed to analyse their variation relation.

第二部分：NDV Sddy-02株F基因的克隆与序列分析本试验设计了一对特异性引物，应用RT-PCR技术扩增出NDV Sddy-02株F基因含裂解位点区363bp片段，同时进行克隆和序列测定分析。

PCI2 cells were treated under glucose deprivation as model group. Method Morphological and cytoflowmetric methods (the mitochondrial transmembrane potential; MEF method) were applied to evaluate the protective effect of SAB. The RNA levels of grp75 were measured by RT-PCR and immunocytochemistry were performed to investigate the expression level of PC12 cells under glucose deprivation and treated by SAB.

以PCl2细胞（大鼠肾上腺嗜铬细胞瘤细胞）的无糖培养建立细胞缺糖损伤模型，MTT法检测细胞的存活率；应用流式细胞仪检测亚二倍体细胞的比率及线粒体跨膜电位的改变；应用免疫细胞化学、RT-PCR法研究细胞内应激蛋白grp75的表达变化，激光共聚焦显微镜观察SAB作用下Ca(上标 2+)的变化。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。