查询词典 RT

RT-PCR result showed that ribozymes could decrease the mRNA amount ofK562/RZ123 cells.

RT-PCR结果表明转染三核酶的K562细胞，其bcr-abl mRNA量明显减少。

RT-PCR analysis revealed higher expression levels of GmRLCK in soybean cotyledons,roots,flowers and young pods;whereas lower expression levels were detected in radicles,stems and mature leaves at vegetative stage.

RT-PCR的结果表明，GmRLCK在大豆子叶、根、花以及豆荚中都有较高的表达，而在胚根、茎和成熟叶片中的表达相对较弱。

In order to elucidate the relationship between the structural features of leginsulin gene in legume plants and their phylogenetic significance,we have cloned the cDNA sequence of leginsulin gene from radicles of broad bean via RT-PCR techniques according to the leginsulin gene sequence we previously obtained from soybean.The cloned cDNA encoded for a precursor protein consisting of the signal peptide,mature leginsulin and an additional 45 amino acids of another polypeptide.

为探明豆科植物中豆类胰岛素基因的结构特征与进化关系，在已获得大豆豆类胰岛素基因的基础上，以蚕豆种子胚根mRNA为材料，采用RT-PCR技术，克隆了蚕豆豆类胰岛素基因的cDNA序列，编码的前体多肽包括信号肽、成熟型豆类胰岛素及另一多肽的45个氨基酸残基。

To provide basic parameters of evaluating the biological safety of xenotransplantation from pig to human, ear tissues from 31 individuals were randomly collected from a Shazi Ling pig population. PCR and RT-PCR were performed to detect porcine endogenous retrovirus proviral DNA and mRNA respectively. The sensitivity of the PCR was evaluated using a positive control.

为评价从猪到人异种移植的生物安全性提供依据，从湖南沙子岭猪的保种群内随机采集31头个体的耳样组织，应用PCR和RT-PCR技术分别检测这些组织中内源性逆转录病毒（porcine endogenous retrovirus，PERV）的前病毒DNA和mRNA，并对PCR扩增的灵敏性进行评估。

The results showed PERV proviral DNA and mRNA could be detected in all 31 individuals by PCR and RT-PCR, respectively. env-A, env-B and env-C were only detected in 2 individuals, while in the other 29 individuals, only env-A and env-B but not env-C was detected. The quantity of DNA in PCR amplification of PERV genes should be more than 15 ng.

PCR和RT-PCR结果表明，所检测的31头沙子岭猪均带有PERV前病毒DNA，耳样组织中均有PERV mRNA表达，其中有2头个体携带 env-A、env-B、env-C 3种囊膜蛋白基因，而其余的29头个体只带有env-A、env-B 两种囊膜蛋白基因，未检测到env-C基因。

Oocyte samples from one group were collected to detect the presence and integration of HBV DNA within cells and chromosomes using PCR, Southern blot, dot hybridization and fluorescence in situ hybridization. The female animals from another group were mated with their normal males, respectively. Their zygotes, 2-cell embryos were collected to detect the integration of HBV DNA in the female pronuclei of zygotes and the replication and expression of HBV genes in the 2-cell embryos using FISH, RT-PCR and immunofluoresence assay.(1) PCR detected positive bands in the tested oocyte samples fromgoldon hamster and mice. Southern blot revealed clear hybridization signals in PCR products.

研究用金黄地鼠和小鼠建立实验动物模型：将卵巢内注射HBV DNA的实验动物分成两组，一组注射后进行超排卵，收集卵巢和输卵管的卵母细胞，用PCR、Southern杂交，斑点杂交和荧光原位杂交(fluorescence in situ hybridization、FISH)检测HBV在卵母细胞内的存在和染色体上的整合；另一组超排雌鼠与正常雄鼠合笼，收集受精卵和2-细胞胚，用FISH、RT-PCR和免疫荧光检测技术分别研究HBV基因在受精卵雌原核上的整合以及在2-细胞胚中的复制与表达。

College of Life Science, Wuhan University, Wuhan 430072On the basis of optimization of in situ RNA hybridization technique, Westudied the temporal and spatial expression of calmodulin and phosphorylasemRNA and its relation with fertilization and embryogenesis in rice as well as antherdevelopment in tabacco. We also constructed cDNA libraries of proembryos anddifferentiating young embryos in rice using microscopic isolation and RT-PCRtechniques.

本文在优化RNA原位杂交技术的基础上，研究了钙调素mRNA和磷酸化酶mRNA时空表达及其与水稻受精和胚胎发生以及烟草花药发育的关系；利用显微分离技术和RT-PCR技术建立了水稻原胚和分化幼胚cDNA文库。

In this experiment we detected changes of PARP mRNA by means of RT-PCR and PARP protein by means of Western-blot.

在本实验中，采用RT–PCR法检测PARP的mRNA的变化和Western-blot法检测PARP蛋白的变化。

For example, don't forget to order self-leveling outriggers if you're using your RT scissor lifts on unimproved surfaces.

例如，如果您将要在坏路面使用RT剪式升降机，请切记要订购自动持平支架。

Besides characterisation of the two genes' function, RT-PCR, Northern analysis, primer extension, and lacZ reporter gene fusions were also tried in this study to investigate the genetic organization of the operons related and their transcription regulation, map gene was found to locate at the end of a long ribosome protein genes operon and yflG is in another operon with its downstream gene.

除了对这两个基因的功能鉴定，本文还阐明了它们各自所在操纵子的遗传结构，并采用RT-PCR、Northern印迹及引物延伸等多种方法，最终分别对它们的启动子进行了定位。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。