查询词典 RT

Gilts were slaughtered on day 12, 25 and 35 of pregnancy, and serum, uterine flushings, embryos and endometria were collected. ELISA was adopted to determine the progesterone concentration in serum and uterine flushings, and RT-PCR was adopted to determine the genes expression in embryos and endometria.

母猪配种后随机分到3个组，按2.0倍、1.2倍和0.6倍维持需要供给饲粮（分别为采食高、中、低水平），妊娠12、25和35d屠宰母猪，收集血清、子宫液和胚胎、子宫组织，运用ELISA测定血清和子宫液中孕酮浓度，用RT-PCR测定目的基因在胚胎和子宫内膜中的表达。

RT-PCR analysis indicated that the TSHβ mRNA was expressed abundantly not only in pituitary but also in gonads.

RT-PCR组织分布分析发现，TSHβ基因除垂体有表达外，性腺也有少量表达。

The expression levels of 7 genes (8#, 9#, 13#, 16#, 8A4R19G1, PSC11 and 1O10) are also checked in different organs such as root, term, leaf, flower and gynophores of peanut, using semi-quantitative RT-PCR.

利用半定量RT-PCR技术分析了8#、9#、13#、16#、8A4R19G1、PSC11和1O10在花生不同器官的表达量。

Total RNAs were isolated from haemocytes of M.rosenbergii and M.nipponense.The cDNAs encoding lysozyme were amplified by RT-PCR.

分别提取罗氏沼虾和日本沼虾血细胞总RNA，RT-PCR扩增获得特异性cDNA片段，纯化后克隆到T载体上。

RT-PCR results revealed that stronger products in the pancreas, liver and head kidney were detected.

RT-PCR结果显示鲢：IL-8 mRNA主要在胰、肝脏和头肾中表达。

Expression analysis with RT-PCR revealed that PcLGBP gene was exclusively expressed in hepatopancreas and the expression could be up-regulated by heat-killed Listonella anguillarum. The binding specificity of PcLGBP to ligand were subsequently examined by recombinant expression of the cDNA fragment encoding its mature peptide in Escherichia coli BL21 (DE3) and ELISA approach.

为分析PcLGBP基因的功能，通过半定量RT-PCR方法研究了其在组织中的分布，并用荧光定量PCR技术分析了其对细菌刺激的响应，结果表明PcLGBP基因是肝胰腺特异表达的基因，能响应热致死鳗弧菌刺激而增强表达，说明PcLGBP基因可能参与螯虾的细菌防御反应。

Methods IUGR model were established by maternal protein-malnutrition. The mRNA levels of TLR4 in hepatics of IUGR rats at 3 weeks of age were analyzed by fluorescent quantization RT–PCR. TLR4 protein levels in hepatics were determined by Western blot. Hepatics TNF-αand IL-1βlevels were measured by ELISA.

采用孕期蛋白质营养不良法建立IUGR大鼠模型，应用荧光定量RT-PCR技术检测3周龄子鼠肝脏中TLR4的mRNA含量变化，Western blot方法检测子鼠肝脏TLR4的蛋白表达，并应用酶联免疫吸附法检测肝组织中肿瘤坏死因子-α和白介素-1β的水平。

objective to investigate whether hypoxia preconditioning exist in adipose tissue,and construct the hypoxemic model of adipose tissue.methods after injected different density cocl2 to rat's subcutaneously of groin,use immunohistochemical method detect hif-1α protein,and use rt-pcr detect hif-1α mrna.results after injected different density cocl2,the adipose tissue of rat's groin express different degree of hif-1αprotein and hif-1αmrna.,and reach the peak when the density is 20-40 mg/kg and the time is 3 h.conclusion hypoxia preconditioning exist in adipose tissue,and the effect of preconditioning is most evidence when the density is 20-40 mg/kg and the time is 3 h.

目的 探讨脂肪组织中是否存在缺氧预适应现象，构建cocl2致缺氧的缺氧模型。方法不同浓度的cocl2大鼠腹股沟区皮下层浸润注射，分别用免疫组织化学法和rt-pcr法检测该区脂肪hif-1α蛋白和hif-1α mrna的表达情况。结果用不同浓度的cocl2行化学缺氧后在不同时间点观察有不同程度的hif-1α蛋白和hif-1α mrna表达，其中，在浓度为20～40 mg/kg，时间为3 h时脂肪组织中hif-1α表达达高峰。结论脂肪组织存在缺氧预适应现象；cocl2的浓度选择20～40 mg/kg，时间选择3 h时缺氧预适应效果较佳。

Progeny viroids in the indicator plant Suyo cucumber were detected by 2D-PAGE,return-PAGE-northern-blot and RT-PCR,and only HSVd can be detected from inoculated cucumber.

通过二维电泳，正反向电泳及Northern印迹杂交，RT-PCR等分子生物学方法对接种的指示植物Suyo黄瓜中的子代类病毒进行了检测，结果仅仅检测到HSVd一种类病毒。

The expression of P2X7 as well as modulation of P2X7 mRNA expression by various inducers in human normal PBMNC was measured by RT-PCR and Immunohistochemical method.

P2X7受体的诱导表达：应用半定量RT-PCR，证明了P2X7在正常人外周血细胞中表达。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。