查询词典 RT

According to the conserved regions of other known insects, a pair of specific primers were designed and the RT-PCR method was used to amplify the partial sequences of HSP70 from cotton aphid, then analyzed the nucleotide and deduced amino acid sequence.

根据已知昆虫HSP70基因的保守性区域，设计特异性引物，采用RT-PCR方法扩增棉蚜HSP70基因的部分序列，测序后进行核苷酸和推导的氨基酸序列分析。

After the stimulation of LPS, the expression of CXCR4 and the concentrations of TNF-α and IL-6 in culture fluid were detected through the methods of Western Blot, immunohistochemistry and RT-PCR respectively.

在LPS刺激肺泡巨噬细胞后，分别应用ELISA、Western Blot及RT-PCR法检测肺泡巨噬细胞培养液中TNF-a和IL-6浓度以及肺泡巨噬细胞CXCR4蛋白和mRNA表达的变化。

Material of the study was FMDV from cell culture, nucleotide sequence of structural gene of FMDV and FMD vaccine strain were acquired by RT-PCR.

本试验以口蹄疫病毒的细胞培养毒为材料，通过RT-PCR方法获得了此株病毒结构基因的核苷酸序列，同法获得了口蹄疫疫苗株结构基因的核苷酸序列。

We have 30t LF refine ladle, are furnace, electric induction furnace, cupola and other melting equipment Furthermore, we have several resin-sand productive facilities and possess RT, UT and other non-destructive test capabilities of casting.

公司现有员工670余人，公司拥有30吨LF精炼炉、电弧炉、中频炉、等冶炼设备以及树脂砂生产线，具有RT、超声波探伤等无损检测能力。

The inhibition of SPG-Rg3 on the growth of MCF-7 cells was detected by MTT assay,IC50 was calculated to obtain the effective concentration; flow cytometry was used to observe the cell cycle of MCF-7 cells; AO/EB fluorescence double-dye technology was performed to observe the apoptosis of cells in morphology,immunocellularchemistry and RT-PCR were utilized to investigate the apoptosis of MCF-7 cells and its relationship with caspase-8 from protein level and molecule level.

采用MTT法观察SPG-Rg3对MCF-7细胞生长的抑制作用，并计算出IC50，依据IC50值确定SPG-Rg3的有效浓度；流式细胞术检测SPG-Rg3作用后MCF-7细胞周期的变化；AO/EB双染从形态学上观察SPG-Rg3对MCF-7细胞凋亡的作用；免疫细胞化学染色和RT-PCR技术分别从蛋白水平和分子水平上检测SPG-Rg3对MCF-7细胞的诱导凋亡作用及其与caspase-8基因的关系。

Methods The model of refractory epilepsy was established by kindling of amygdale. The expression of PGP in cerebra was observed by RT-PCR, immunohistochemistry and western blot.

方法建立杏仁核电刺激点燃的难治性癫癎大鼠模型，用RT-PCR、免疫组织化学及免疫蛋白印记的方法，分析比较PGP蛋白在癫癎模型组与正常对照组的表达。

In all kinds of matching condition, the lengths of stimulus onset asynchrony have no affects to the RT.

在各种匹配条件下，SOA的长短均没有影响被试的RT。

In the study, RT-PCR and sequencing were employed to detect the expression levels of CCK-B receptor, CCKBRi4sv and gastrin mRNAs in 30 human gastric carcinoma and their corresponding normal tissues, 10 gastritis and 2 autopsied normal stomach specimens as well as in a gastric carcinoma cell line SGC-7901 cells.

本研究首先用RT-PCR及测序方法检测了30例人类胃癌组织及癌周正常胃粘膜、10例胃炎、2例尸检胃组织及胃癌细胞株SGC-7901中CCK-B受体、CCK-B受体的异位剪接变异体(intron 4-contained variant of cholecystokinin B receptor，CCKBRi4sv)及胃泌素基因的表达。

Second is studying the traumatic condition changes in a choosey pressure at different post-PT phases. Moreover, we detected model PLA_2 activity, express of PLA_2 subtype mRNA by fluorescent quantitative RT-PCR technique, protein express and site by Western blot and IHC at different post-PT phases. At last, we studied the PLA_2 inhibited effects and model protection of Variabilin in vivo.

研究在不同压强的外力作用下，模型指定时相点的一般情况和存活率、大体、光镜及电镜下胰腺组织的病理变化和常规酶学变化，得到稳定建模致伤压强；然后，固定致伤压强，研究胰腺创伤后不同时相点的伤情变化(包括一般情况、胰腺组织的病理变化、AMS和LPS活性变化、血常规、血生化、胰腺细胞凋亡以及胰腺组织细胞生长周期变化情况)；再次，采用3H-花生四烯酸标记大肠杆菌膜作为底物的方法检测大鼠胰腺创伤后不同时相点的PLA_2活性，利用荧光定量RT-PCR技术检测其亚型mRNA的表达，利用Western blot和IHC技术检测其蛋白表达量和蛋白组织定位；最后，将Variabilin体内应用于急性胰腺创伤大鼠模型，研究其对模型的保护作用和对PLA_2的抑制效应。

The expression of M1 and M4 subtype receptor in the retina, choroids and posterior sclera were detected with immunohistochemistry and the mRNA level of two receptors in the three tissues were examined via RT-PCR.

实验结束后，通过RT－PCR对各组巩膜、脉络膜和视网膜内M1和M4亚型受体的mRNA水平进行检测，同时使用免疫荧光法检测两种受体在各个组织中的表达情况。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。