查询词典 RT

Two pairs of primers were designed based on the sequence of ovis aries preprochymosin cDNA reported in GenBank.The preprochymosin cDNA was obtained from toltal RNA isolated from the abomasum of 5-day-old Xinong Saanen Goat by RT-PCR method and then cloned successfully in E.coli.

参照GenBank登录的绵羊凝乳酶原前体cDNA序列设计引物，以新生5d的西农萨能羊皱胃组织总RNA为模版，通过RT-PCR方法获得凝乳酶原前体cDNA，克隆测序后进行序列比对。

The Omp25 gene of wapiti's Brucella abortus was amplified by RT-PCR and sequenced, Then the interesting gene was subcloned into pET28a for expression. The results showed that the full length of Omp25 gene was 642bp, which encoded 213 amino acids.

运用RT-PCR技术从马鹿布氏杆菌分离株中扩增出Omp25基因，将其克隆入pMD18-T中进行核苷酸序列测定和遗传变异分析，并将目的基因亚克隆到大肠杆菌表达载体pET28a中诱导表达。

RT-PCR analysis showed that the ZmASK1 expression was induced by mannitol, salt and abscisic acid.

半定量RT-PCR分析表明，ZmASK1可以被甘露醇、高盐和脱落酸诱导。

RT-PCR analysis showed that the ZmPti1 expression was induced by salicylic acid, low-temperature, mannitol and salt but not by abscisic acid.

半定量RT-PCR分析结果显示ZmPtil基因的表达可以被水杨酸、低温、甘露醇和盐所诱导，但是不被脱落酸所诱导。

Then, two primers were designed from it, the full length cDNA of malate dehydrogenase gene in Chinese cabbage was gained through RT-PCR amplifying and sequencing and entered GenBank as accession number FJ208590, which is 1 209 bp length and coded 403 amino acids.

然后设计引物，经RT-PCR扩增、测序，获得了大白菜苹果酸脱氢酶基因的cDNA全长序列，并已将其登录到GenBank，登录号为：FJ208590，该cDNA全长1 209 bp，编码403个氨基酸。

The competitive RT-PCR used in this study overcomes the limitations of β-actin method.

研究中选用竞争RT-PCR方法克服了以往使用β-actin的局限性。

K562 cell cultivated in suspension and adhesively cultivated with msc were collected respectively and cell proliferation curves were drawn; the cell cycle was determined by flow cytometry; the effect of chemotherapy on cellular viability and apoptosis of k562 cell was investigated,the mdr1 gene expression was determined by rt-pcr.

对悬浮培养和与msc黏附培养的k562细胞绘制细胞生长曲线；用流式细胞术测定细胞周期并观察化学药物对细胞存活率和凋亡的影响；用rt-pcr技术检测mdr1基因表达。

K562 cell cultivated in suspension and adhesively cultivated with MSC were collected respectively and cell proliferation curves were drawn; the cell cycle was determined by flow cytometry; the effect of chemotherapy on cellular viability and apoptosis of K562 cell was investigated, the MDR1 gene expression was determined by RT-PCR.

叶悬浮培养和与MSC黏附培养的K562细胞绘制细胞生长曲线；用流式细胞术测定细胞周期并观察化学药物对细胞存活率和凋亡的影响；用RT-PCR技术检测MDR1基因表达。

A is the central administrative domain of RT; it keeps things organized.

队列是RT的首要的管理的领域；它使得事情有条理。

The level of GR mRNA expression in hepatic cytosol were determined by RT-PCR. Plasma adrenocorticotrophic hormone and glucocorticoid concentrations were determined by the radioimmunoassay.

以[^3H]地塞米松为配体，用一点分析法测脑和肝胞液GR结合活性、半定量RT—PCR方法测肝胞液GR mRNA水平、放免法测血浆促肾上腺皮质激素和皮质酮浓度。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。