查询词典 RT

Specific primers were designed according to gene NHX1 of Limonium gmelinii published on NCBI. Then, the gene was cloned by means of RT-PCR. Eukaryotic expression vector pCAM-BIA1301-NHX1 had been constructed, then transformed into tomato by Agrobacterium-mediated transformation method. Transgenic tomatos were obtained.

根据NCBI公布的大叶补血草NHX1基因序列设计特异性引物，经RT-PCR方法克隆得到大叶补血草液泡膜Na/H逆向转运蛋白基因NHX1，构建植物融合表达载体pCAMBIA1301-NHX1，重组质粒通过农杆菌介导转化番茄，经PCR和RT-PCR鉴定，获得转基因番茄植株。

To eplore the relationship between Dynein and the formation of meiosis error of oocytes, the immature mouse oocytes were in vitro cultured with a known dynein ATPase activity inhibitor- sodium orthovanadate to detect the changes of maturation rate, and semi-quantitative RT-PCR and single cell RT-PCR were performed to detect the alteration of the cyclin B1 mRNA level.

为探讨Dynein与卵母细胞减数分裂错误形成的关系，采用小鼠卵母细胞体外培养成熟技术和半定量RT-PCR方法，分别检测Dynein抑制剂-原钒酸钠对卵母细胞成熟率和cyclin B1基因转录水平的影响。

Cells were immediately fixed and processed for immuno?uorescence and tested for translocation ofβ-catenin.β-catenin and target genes of Wnt/β-catenin signaling COX-2, cyclinD1, c-fos, c-Jun mRNA expression were examined by RT-PCR. Saos-2 osteoblastic cells were co-transfected with a wild type or mutant Tcf luciferase reporter gene and Renilla luciferase plasmid. Posttransfection cells were subjected to 1 hour of 12% elongation.

作用1h、2h和4h后，用免疫荧光的方法检测β-catenin的表达和在细胞上定位的变化；用半定量RT-PCR检测β-catenin mRNA表达的变化；用质粒转染的方法检测荧光素酶报告基因Tcf转录的情况；用半定量RT-PCR检测Wnt/β-catenin通路下游基因COX-2，cyclinD1， c-fos， c-Jun mRNA表达的情况；用免疫共沉淀的方法检测β-catenin和E-Cadherin的结合情况。

In order to establish the detection of Hemagglutinating Encephalomyelitis Virus by RT- PCR, a pair of oligonucleotide primers appropriate for PCR amplication was selected based on the HE gene and S gene of HEV.

为了建立快速检测猪血凝性脑脊髓炎病毒的RT-PCR方法，根据GenBank中HEV的HE基因和S基因设计了1对引物，在优化RT-PCR反应条件的基础上，成功的扩增出323bp的特异性条带。

METHODS: Sixty two cases of specimens collected in operation were studied by in situ RT PCR, of which 50 cases were hemangioma: 26 cases proliferating hemangioma and 24 cases involuting hemangioma, and 12 cases were venous vascular malformation. The expression of Ang2 mRNA and Tie2 mRNA in hemangiomas and venous vascular malformation were detected by in situ RT PCR.

采用原位RT PCR方法，对临床收集的62例手术切除的先天性皮肤血管标本（其中增生期血管瘤26例，消退期血管瘤24例，静脉型血管畸形12例）进行Ang2 mRNA， Tie2 mRNA表达水平的检测。

Esults LVSV and RVSV determined by RT-3DE were (15.90±10.62) ml and (12.42±4.69) ml respectively, which were shown good correlation with those determined by MRI [(16.56±10.64)ml and (13.96±7.96) ml]. The r value was 0.98, and 0.75, respectively. LVSV and RVEV determined by RT-3DE were (12.67±10.10) ml and (11.83±12.02) ml respectively, which were well correlated with MASE (8.17±1.92)mm and TASE (11.04±1.06) mm determined with ME. The r value was 0.86 and 0.88 respectively.

T-3DE测定左室每搏量(12.67±10.10)ml和ME测定二尖瓣瓣环收缩期平均位移(8.17±1.92)mm之间的相关性显著，相关系数r=0.86(P.001)；RT-3DE测右室每搏量(11.83±12.02)ml和ME测定三尖瓣瓣环收缩期位移(11.04±3.06)mm两者亦存在显著正相关，相关系数r=0.88(P.001)。

CEVd is a non-polyadenylated RNA viroid,but there is a fragment rich in A in CEVd sequence.On the bases of uniplex RT-PCR, a multiplex RT-PCR was developed to detect CTV,CEVd and CTLV simultaneously.

实验过程中，发现不具有ploy_加尾的环状类病毒CEVd，同样可以用oligo反转录的RT-PCR检测出来，通过测序分析，其同源性在96%以上，并发现在CEVd序列中有一个富含A的区段。

Objective To establish a competitive RT-PCR method for quantitative determination of IFN-γ mRNA and IL-4 mRNA and explore the optimal inductive condition in vitro. Methods IFN-γ mRNA and IL-4 mRNA in PBMC induced by PMA and calcium ionophore of a healthy volunteer and in unstimulated PBMC of 11 HVs and 13 asthma patients were determined quantitatively with the established competitive RT-PCR method using self-prepared internal standard as competitive templets.

Subject words 】 Competitive templet； IFN-γ； IL-4； mRNA； RT-PCR 检测细胞因子最早使用生物学或免疫学测定法，这两类检测可反映某种细胞因子分泌、吸附、消耗和降解后的净含量〔1〕，但不能为细胞因子产生过程中基因转录、翻译及翻译后调控机制提供必要的信息，也不能反映细胞或组织内细胞因子基因表达水平。

The same 20 viruses have been previoushy typed using serotyping method resulting that only 11 could be typed successfully, showing a high consistency with that of RT-PCR-RFLP method and a 30% lower typing efficiency than the latter.

此20个毒株曾用血清学方法分型，仅11株分型成功，与RT-PCR-RFLP法结果符合。分型成功率RT-PCR-RFLP法比血清法高30%。

In order to elucidate the regulation mechanism of RU5 region to BFV gene expression, BFV3026 provirus DNA was used to construct the plasmids containing different deletion of R region, which were cotransfected with luc report gene locatied behind the IP promoter to BL12 cells, and luciferase activities was assayed, confirming that U5 region could repress the initiation function of LTR as well as IP. The BFV structure genes with different deletion of R region were cloned closely behind to heterogenous CMV promoter, then transfected to 293T cells, RT activity was performed, testifying the R region was required for BFV pol gene expression, and also the function domain was identified within the 100n.t. sequence at the 5′end.

以牛泡沫病毒(Bovine foamy virus， BFV)中国株BFV3026原病毒DNA为材料，构建R区系列缺失质粒，通过对其转染细胞中RT水平及对缺失质粒与luc报告质粒共转染细胞中萤火虫荧光素酶活性的测定，确立U5区对于BFV3026两类启动子LTR和IP均具有负调控作用；同时将带有不同R区的BFV3026结构基因片段克隆于异源启动子CMV之下，通过对其转染细胞293T中RT酶活性的测定，确立R区对于病毒结构基因pol的表达具有一定的调节作用，并将其功能区域初步界定在R区5′端100bp内。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。