查询词典 RT

Expressions of cytokines in lung tissue were measured by RT-PCR. Pulmonary neutrophilic infiltrate was measured by the myeloperoxidase assay.

RT-PCR法检测肺组织内细胞因子的表达，髓过氧化物酶法测定肺组织中性粒细胞聚集程度。

The RNAs synthesized in PK15 cells of GD and BF strain, representative strain of PCV2 dominant genotype (CHN-2H) and nondominant genotype (CHN-2A) respectively, were characterized by Northern blot and RT-PCR.

采用Northern blot和RT-PCR技术对PCV2优势基因型(CHN-2H)的代表毒株和非优势基因型(CHN-2A)的代表毒株进行了PK15细胞培养的基因转录分析。

Methods Malignant tumor tissues were obtained from 11 patients with colon cancer, 15 patients with cervical cancer, 10 patients with breast cancer, 9 patients with esophageal cancer, 12 patients with lung cancer and 16 patients with gastric carcinoma. Nonmalignant tissue was used as negative control. Three tumor cell lines were also studied. Hfgl 2 was examined both at the level of mRNA and protein by immunohistochemistry as well as RT-PCR.

采用免疫组化方法检测了11例结肠癌、15例宫颈癌、10例乳腺癌、9例食管癌、12例肺癌、16例胃癌患者的病理组织切片中hfgl 2及纤维蛋白的表达，分析二者的组织学关系，用RT-PCR方法检测了部分肿瘤组织与肿瘤细胞系中的hfgl 2 mRNA。

Light microscope and transmission electron microscopy showed that SMMC-7721 cells induced by SAHA had undergone the restorational alteration in morphology and ultrastructure, which were different from those of nontreated cells but were similar to those of normal cells, and the changes were as follows: the cells turned to be flat and spread; the nucleo-cytoplasmic ratio lessened and nuclear shape became rather regular; the number of nucleolus reduced and its volume lessened; euchromatin increased while heterochromatin decreased in nucleus; in the cytoplasm, mitochondria grew in number with relatively consistent structure and well-developed mitochondria cristae; Golgi complex turned to be well-developed and typical; rough endoplasmic reticulum increased. Immunocytochemistry assay showed that the expression of AFP and PCNA were declined significantly. FCM analysis showed SAHA could arrest SMMC-7721 cells in G0/G1 phase, with an accumulation of the cells in G0/G1 phase while a decrease of cells in S phase. Semi-quantitative RT-PCR detection revealed that the expression of p21WAFl mRNA was upregulated remarkably in the cells treated with SAHA.

结果：倒置显微镜和透射电镜观察显示，经SAHA处理的细胞增殖速度显著减慢，细胞体积增大，细胞核较小，形状较为规则，核仁数量减少、体积变小，核内常染色质增多而异染色质减少，核质比例减小，细胞质内线粒体数量增多、线粒体嵴发达，高尔基体较为典型，粗糙型内质网增多，呈现出与正常上皮细胞相似的形态变化；MTT比色法测定结果显示不同浓度(2.5、5.0、7.5、10.0uM)SAHA对SMMC-7721细胞的增殖均有抑制作用，并有明显的剂量依赖和时间依赖关系；免疫细胞化学检测显示SAHA能显著降低PCNA和AFP在SMMC-7721细胞中的表达；流式细胞仪检测结果显示，SMMC-7721细胞经SAHA处理后，G0/G1期细胞明显增加，S期细胞则明显减少，细胞被阻滞于G0/G1期；RT-PCR检测结果表明，SAHA作用12h后SMMC-7721细胞中p21WAF1 mRNA的表达即有增加，24h后更为明显。

In contrast,the expression levels of Lb est2 in nymphal stages are lower than that in adult stage.2 Gene cloning of AChE and mRNA expression level in L.entomophila2.1 Gene cloning and sequence analysis of AChE in L.entomophilaTwo full length cDNA encoding AChE were cloned from L.entomophila by the methods of RT-PCR and RACE,named Le ace1(GenBank Accession No.:EU854149) and Le ace2GenBank Accession No.

CarE基因在不同发育阶段mRNA表达量的分析结果表明，Lb est1的表达量随着该虫生长发育的进行逐渐降低，至成虫期时表达水平最低；相反，L6 est2在若虫期表达水平较低，而在成虫期表达水平最高。2嗜虫书虱乙酰胆碱酯酶基因克隆及其mRNA表达水平研究2.1嗜虫书虱乙酰胆碱酯酶基因克隆及序列分析利用RT-PCR和RACE技术成功克隆获得嗜虫书虱2个AChE基在的全长序列，分别命名为Le ace1(GenBank登录号：EU854149)和Le ace2(GenBank登录号：EU854150)。

2Semi-quantitative RT-PCR was conducted to compare the expression of TNF-α gene in omental adipose tissue of obesity and insulin resistance patients with normal individuals.

2用半定量RT-PCR方法研究两组大网膜脂肪组织中TNF-α基因的表达。

RT-PCR analysis showed that both of the two genes were expressed in all tissues in Oryza sativa L.

RT-PCR分析表明OsFAD2和OsFAD6在所有器官中都表达，在叶中表达量为最高。

Five sheep were sacrificed at 3, 4 and 6 weeks after osteotomy, respectively, and specimens were harvested for detecting the expression of TGF-β1 and IGF-I by immunohistochemistry and RT-PCR.

术后3、4和6周分别处死5只动物，应用免疫组织化学与RT—PCR检测骨痂组织中TGF—β1与IGF—Ⅰ的表达。

RT-PCR detection of SYT-SSX mRNA can be used in formalin-fixed, paraffin-embedded tissues as a sensitive and specific technique in the diagnosis and differential diagnosis of synovial sarcoma. 2. The SYT-SSX fusion type is related to histological type and cell proliferative activity of synovial sarcoma. 3. Some characteristic sequence motifs flank genomic breakpoints of chromosomal translocation of synovial sarcoma, including Alu sequences, Translin recognition sequences, topoisomerase Ⅱ recognition sites and palindromic sequences, which may be associated with chromosomal translocation of synovial sarcoma.

1、RT-PCR技术检测SYT-SSX mRNA表达可用于福尔马林固定、石蜡包埋组织，敏感性和特异性高，可用于滑膜肉瘤诊断和鉴别诊断。2、SYT-SSX融合基因类型与滑膜肉瘤组织学分型及细胞增殖活性相关。3、滑膜肉瘤染色体易位断裂点旁存在一些特征性序列，包括Alu序列、易位素识别序列、拓扑异构酶Ⅱ识别位点、回文序列等，可能与其染色体易位发生机制有关。

The acceleration profile of the crane motion is designed to induce minimum payload pendulation during transpo rt,while the deceleration stage is designed to ensure freed om of residual oscillation near the end of the transport,and to deliver the payload at the target point.The techniques induce payload pendulation at least one half ofan oscillation cycle or integer multiples during each of the acceleration and deceleration stages.Starr(1995)designed a symmetric two-step acceleration and deceleration profile to transfer a suspended object.The duration on the constant acceleration steps is assumed to be negligible relative to the period of the payload oscillation.A linear approximation method of the oscillation period is used to generate the two-step symmetric acceleration profile.

3.1投入成型技术，各种形式的投入shapers ， convolved参考命令，产生形投入指挥，驾驶起重机提出一套距离沿一套path.the加速度概况起重机议案旨在诱使最低载重pendulation期间transpo rt ，虽然减速阶段，目的是确保freed om残余振荡接近尾声的运输，并交付载荷到达目标point.the技巧诱使载荷pendulation至少一半ofan振荡周期或整数倍数。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。