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RT-PCR analysis showed the GhMASD1 gene expressed in petals, stamens, ovules and fibers, but not in roots,stems, leaves, bracts and sepals. The strongest expression of GhMADS1 gene was detected in petals. But in floral buds of a cotton homeotic mutant (CHV1), whose floral orgens are all converted to bract leaf-like organs, the transcript of GhMADS1 gene was not detected.

RT-PCR分析显示，该基因在陆地棉的花瓣、雄蕊、胚珠和纤维中表达，特别是在花瓣中表达量最高，而在根、茎、叶等营养器官和棉花同源异型突变体CHV1（所有花器官均变为苞叶状叶性器官）的变异花蕾中不表达。

The plasticity of NSCs was detected by the differentiating test. HESCs were induced into dopaminergic neurons by means of adding Sonic Hedgehog Peptide and fibroblast growth factor eight (FGF8) early under the condition of mocked microenvironment and different development stage of dopaminergic neurons in vivo ,meanwhile made comparison with traditional method . The marker of dopaminergic neurons was tested by immunocytochemistry and RT-PCR, the content of dopamine and its derivation homovanillic acid were measured by HPLC-ECD.

模拟体内多巴胺神经元分化发育的不同阶段及微环境，采用早期添加音猥因子及成纤维细胞生长因子-8(FGF8)的方法，诱导hESCs定向生成多巴胺能神经元，并与传统的诱导方法相比较，免疫荧光细胞化学和RT-PCR法检测多巴胺能神经元的标志，高效液相色谱法检测诱导后细胞及细胞培养上清液中多巴胺及其代谢产物高香草酸的含量。

Based on the degenerated primer, the cDNA of open reading frame of growth horme gene for black carp and Japanese crucian carp were cloned and sequenced through RT-PCR, which were used for the"all-fish"transgene construction and preparation of polyclonal antibody against GH for black carp.

基于鲤科鱼类生长激素基因序列，通过RT-PCR技术，克隆了青鱼、日本白鲫生长激素基因编码区cDNA序列，以此来构建&全鱼&基因载体和研制生长激素多克隆抗体。

Protein moleculars interacted with Plk1 were screened with yeast two hybridation system, Plk1 cDNA was amplified from total RNA of murine embryonic stem cells by RT-PCR.

我们选择了实验室现有的小鼠胚胎干细胞cDNA文库作为基础，首先利用RT-PCR方法获得了小鼠Plk1基因。

GRP can also make mice's MNC and hematopoietic stem cells from human's umbilical cord blood amplification. We use colony formation to analysis the linear relation during the cells'growth and the medicine's dosage, examine the amplification of CFU-GM induced by hypothallus and its supernatant, RT-PCR is used again to check three cytokines'genes.

发现GRP对小鼠MNC扩增有作用，从而将GRP运用到人脐带血MNC的扩增分化上，用集落形成法分析药物与扩增的线性关系，基质层、基质上清对CFU-GM的影响，用RT-PCR技术检测基质层细胞生长因子基因的扩增。

The rearranged idiotypic CDR3 fragments coding TCR Vβ8 of the Jurkat cell strain and TCR Vβ2 of the Molt4 cell strain were amplified using RT-PCR, and then were cloned into pIRES vector respectively.

用RT-PCR方法扩增特异性重排的Jurkat细胞TCR Vβ8 CDR3序列和Molt4细胞TCR Vβ2 CDR3序列，分别克隆到真核表达载体pIRES中。

Ovine immatured oocytes, matured oocytes and embryos from 2-cell stage to blastocyst produced in vitro were recovered, and RT-PCR was used to semi-quantify mRNA expression. Proteins of Cx43 and Cx45 were detected by using a combination of immunocytochemistry and Laser Scanning Confocal Microscope.

收集绵羊未成熟和成熟卵母细胞以及体外受精早期胚胎各发育阶段的卵裂球细胞，通过RT-PCR半定量检测Cx43和Cx45基因的mRNA表达量；通过免疫组织化学方法结合激光扫描共聚焦显微镜检测Cx43和Cx45蛋白在绵羊体外受精的早期胚胎发育过程中的表达情况及表达区域。

The sandwich ELISA assay showsbetter sensibility and specificity than RT-PCR,hemagglutination inhibition assayand indirected ELISA.

与RT-PCR和间接ELISA法相比，双抗体夹心ELISA法具有敏感性高、特异性强的优点，适于临床检测使用。

METHODS: Thirty six SD female rats were randomly separated into 3 groups (n=12 in each one) according to the infarction duration (1, 3, 28 d), which were randomly and evenly again separated into 2 subgroups: operated group and sham operated group. The levels of mRNA expression of ACE2 in the noninfarct and infract area of left ventricle 1, 3, 28 d after AMI were detected by RT PCR and analyzed semi quantitatively by image analysis system.

将36只SD雌性大鼠按时间段随机分为3组（每组12只），每组再随机分为假手术组、手术组2个亚组（每组6只），应用RT PCR方法检测大鼠AMI后1，3，28 d左心室梗死区及非梗死区心肌ACE2 mRNA的表达，并以图像分析系统对其进行半定量分析。

Total RNA was extracted from venom cells from Gloydius intermedius, FLE gene was amplified by RT-PCR. Then it was connected to pMD18-T vector by TA cloning. At last, sequencing and analysis the genesequence.

从中介蝮蛇毒腺细胞中提取总RNA，通过RT-PCR法扩增纤溶酶基因，与pMD18-T载体连接进行TA克隆，再进行序列的测定并且对该序列进行分析。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。