查询词典 RT

Compared with rt-PA, r-PA has less influence on the cruor system and fibrinolysis system.

静脉注射r-PA对兔RVO模型有治疗效果，疗效与rt-PA无明显区别，r-PA对凝血和纤溶系统的影响比rt-PA小。

The methods for synthesis of RT base have been sumed up- The synthetic processof RT base from benzeneamine and diphenylamine have been detailed.

本文综述了 RT培司的现有各种合成方法。

Methods The LPL cDNA was isolated from the human epiploon adipose tissue by means of RT-PCR. The LPL cDNA was ligated into the pcDNA3.1Zeo. The recombinant pcDNA3.1Zeo-LPL cDNA was identified by endonucleases, PCR and DNA sequencing. COS-1 cells were transfected with the recombinant LPL gene plasmid using Lipofectamine 20001M The LPL mass in cells and the culture medium were determined by a Markit-M LPL Kit. Spectrophotometry was used to measure the LPL activity.

采用RT-PCR法从人大网膜脂肪组织获取LPL cDNA基因，以pcDNA3.1Zeo质粒作为载体，运用基因克隆技术构建野生型人LPL cDNA重组质粒，限制性内切酶酶切、PCR以及双向测序鉴定重组质粒；运用Lipofectamine 2000将重组pcDNA3.1 Zeo-LPL cDNA质粒导入COS-1细胞，RT-PCR法检测LPL mRNA，ELISA法和比色法分别检测细胞裂解液及细胞培养基中LPL浓度及活性。

Establishment of a rapid, accurate and sensitive one-step IC-RT-PCR method to detect Bean pod mottle virus in soybean seed.

建立快速、准确、简便的检测大豆种子上菜豆荚斑驳病毒的免疫捕获一步RT-PCR方法（一步IC-RT-PCR）。

Methods The expressions of MAGE-3 in tumor tissue, para-tumor tissue of (5±1)cm from border of tumor tissue, tissue of resection border and polypus were detected by RT-PCR. Results MAGE-3 expression was positive in 14 of 33 (42.42%) samples of rectal cancer, 6 of 33 (18.18%) of para-tumor tissue, and 5 of 33 (15.15%) of resection border tissue respectively.

采用RT-PCR法，对33例直肠癌患者的癌组织及其相应距肿瘤边缘(5±1)cm的癌旁组织和手术切缘以及3例直肠息肉标本的MAGE-3的表达情况进行检测，并对RT-PCR扩增产物中的目的基因片段进行DNA测序验证。

The number of differentially expressed genes was 71 at 24h, and 62 at 72h. The metabolisic gene class was the majority among all the differentially expressed genes, and the next was transportational、differentiation and adhesional gene class by turns.1.2. RT-PCR verification of the known HIF-1 targeted genes and proliferation related genesIn the 131 differentially expressed genes, we found 7 known HIF-1 targed genes (ldha、aldoa、eno1、gapdh、tpi1、pgk1、bnip3) and 5 genes (dnaja2、ccnb1、tgfβ2、ccng1、rgs7) which were related to proliferation and cell cycle.

对差异表达的基因进行基因功能分类发现：代谢类基因最多，其次是运输类基因、细胞分化及黏附类基因。1.2 RT-PCR验证HIF-1靶基因和增殖相关的基因由于我们的前期实验证明了低氧促NSC增殖是有HIF-1参与的，我们对131个差异表达基因中，7个已知的HIF-1靶基因(ldha、aldoa、eno1、gapdh、tpi1、pgk1、bnip3)和5个与细胞周期和增殖相关的基因(dnaja2、ccnb1、tgfβ2、ccng1、rgs7)做RT-PCR验证。

RT-PCR showed that VEGF-C mRNA expression in Crocin treated group was significantly lower than that of the control group. The cDNA microarray analysis was coincided well with the RT-PCR data.

RT-PCR显示藏花素组VEGF-C表达明显减少，基因芯片扫描结果中藏花素组VEGF-C表达也显著下调。

The RT-PCR product was inserted into pTG19-T vector and transformed into E. coli successfully. By blastn, the sequence results of Kunming mus musculus were in complete accordance with the conservative sequence of Genbank NR_003278 (791bp-1153bp). By Blastn in NCBI, the sequence with little difference among animals was confirmed to be conservative. After Blastn, fourteen complete CDS coding for different animals were chosen. According to VECTOR NIT 9.0 software, the similarities between Kunming mus musculus and bos taurus, homo sapiens, erinaceus europaeus, cricetulus griseus, sus scrofa, dasypus novemcinctus, rattus norvegicus, rabbit, equus caballus, macaca fascicularis, didelphis virginiana, monodelphis domestica and vombatus ursinus was 67%, 100%, 100%, 36%, 100%, 100%, 67%, 100%, 100%, 92%, 99%, 99% and 99%. In the phylogenetic tress constructed with the forteen 18S rRNA by Treeview, the Kunming mus musculus clustered with cricetulus griseus, sus scrofa and rabbit, which was nearer to cricetulus griseus and was most far away from macaca fascicularis.(3) After sencodary structure analyses of 18S rRNA of mus musculus, an oligonucleotide fragment for RNAi was designed and synthesized, which was transformed into plasmid, and restriction enzyme analyses and sequencing results should the expression plasmid pGPH1/ GFP/Neo-mouse-sh 18S rRNA were constructed for RNAi successfully.

结果①通过RT-PCR检测显示18S rRNA基因在小鼠卵巢组织和单个GV期、MⅠ期卵母细胞中均有表达，且在未成熟卵母细胞中，MⅠ期的表达明显强于GV期的表达；②RT-PCR产物克隆测序结果显示：昆明小鼠18S rRNA基因保守区序列与基因库序列[NR_003278保守区部分(791bp～1153bp)]完全一致；Blastn比对结果发现：在不同物种中差异较小，选出14种生物18S rRNA全序列经VECTOR NIT 9.0软件分析，提示昆明小鼠18SrRNA与牛、人类、刺猬、中国仓鼠、猪、犰狳、褐鼠、兔子、马、食蟹猴、负鼠、短尾猊、袋熊的18S rRNA的相似率依次为67%，100%，100%，36%，100%，100%，67%，100%，100%，92%，99%，99%，99%；Clustal 1.81和Treeview构建出的分子进化树表明：在上述14种生物中昆明小鼠与中国仓鼠进化关系最近，与兔子、猪聚成一簇，与食蟹猴进化关系最远；③根据18S rRNA二级结构设计并合成RNA干扰寡核苷酸片段，重组质粒经过限制性内切酶及测序表明成功构建了pGPH1/GFP/Neo-mouse-sh 18S rRNA干扰表达质粒。

The high sensitivity and specificity of real time RT-PCR were shown by The test result, 10~7 of the diluted allantoic fluid infected by AIV can also be detected, and has no cross-reaction with common Avian virus and Avian vaccines, such as low virulent NDV and IB V vaccine.5 days after the broiler has been injected with AIV vaccine, no matter hypodermal or muscular injection, the real time RT-PCR has not found any AIV nucleotide, indicating the use of AIV vaccine has no effect on the test results.

在检测禽流感感染的尿囊液时，尿囊液稀释至10~(-7)仍可检出病毒核酸，荧光RT-PCR方法的特异性也很强，检测时与禽群常用的疫苗如新城疫弱毒疫苗、传染性支气管炎弱毒疫苗等均不出现交叉反应。

Despite evidence implicating prostaglandins as critical factor in male reproductive functions, little is known about expression and function of COX-2 in the male reproductive system. By using immunohistochemistry, RT-PCR and Western-blot, the content of this research included:(1) The expression and localization of COX-2 in mature rat and human testis were examined.(2) We investigated the changes of spermatogenesis and testosterone levels in testis after by using the COX-2 specific inhibitor-rofecoxib at 2, 4 and 8 weeks.(3) To explore the relationship between the intratesticular level of testosterone and expression of COX-2 by completely destroying the Leydig cells of mature male rats with injection of a single I.

本课题应用免疫组织化学、RT-PCR和Western-blot等方法检测以下内容：(1)在mRNA和蛋白水平检测正常成年男性及大鼠睾丸组织中COX-2的表达及定位情况；(2)实验组大鼠持续给予COX-2酶的特异性抑制剂Rofecoxib，分别于用药后2周、4周和8周检测大鼠的精子生成以及睾丸组织内睾酮浓度的变化；(3)另一组实验大鼠给予特异性Leydig细胞灭活剂EDS，在特定时间内抑制睾丸组织内合成的睾酮，于用药后2周和4周，采用免疫组织化学及RT-PCR方法观察大鼠睾丸组织内睾酮浓度变化对COX-2合成的影响。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。