查询词典 RT

RT-PCR results of LCM showed that it's expressed in the primary and secondary spermatocyte.

激光显微切割RT-PCR结果显示Cklfsf1表达于睾丸组织的初级和次级精母细胞。

To detect effects on apoptosis of cortex neuron in DHCA rats, the rat model of occluding bilateral common carotid artery in the deep hypothermic (21C) rats had been established, and flow cytometry, TUNEL, electron microscope, immunohistochemistry and RT-PCR had been studied.: part I: To establish a new rat model of cerebral ischemia/protect during DHCA: 20 SD rats were used in the experiment, little animal respirator was to assist anesthetized rat's respiration after tracheal cannula, artery press through artery spile in femoral artery, electrocardiograph, temperature, blood saturation of O2 (SaO2) and arterial blood gases were monitored during the whole experiment, blood flow of bilateral common carotid artery of rat were occluded about 60 minute when its temperature was reduced to 21 C by ice, then normal temperature was resumed.

本研究采用大鼠深低温颈总动脉阻断模型，应用流式细胞仪、电镜、原位缺口末端标记法、免疫组化法和RT-PCR技术分别检测深低温脑缺血对皮层神经细胞调亡的影响方法：第一部分深低温停循环脑保护大鼠模型的建立：SD大鼠20只，麻醉后气管插管辅助呼吸，用冰块将大鼠的体温降至(21±1)℃，阻断双侧颈总动脉，记录体温、心率、血压、血氧饱和度和血气分析，60分钟后恢复颈总动脉血流，用Longa等的5级评分标准评定4小时和24小时后大鼠神经系统损伤情况。

Methods Entire coding sequence of CSP was reverse transcribed and amplified by RT-PCR with sporozoite total RNA as template,then cloned into pFLAG-CMV8 for construction of the recombinant plasmid pFLAG-CMV8-CSP.

以约氏疟原虫BY265株子孢子总RNA为模板，用RT-PCR扩增CSP基因的编码区序列并克隆至pFLAG-CMV8载体，构建重组质粒pFLAG-CMV8-CSP。

Total RNA was isolated from antler tip, the growing antlers from 3-4 years spotted deer were removed on the 30th day after the mineralized ones shed, and the cDNA sequence of GHR gene was cloned by RT-PCR technology.

以3~4岁健康鹿生长30 d的鹿茸顶端组织提取的总RNA为模板，利用RT-PCR技术克隆GHR基因的cDNA序列；并用原位杂交技术对GHR基因在鹿茸顶端进行组织定位。

The transformed plants were selected by Kanamycin,and PCR analysis and RT-PCR analysis preliminarily proved the stilbene synthase gene had been integrated into the tobacco genome and could transcript normally.

经卡那霉素筛选、PCR、RT-PCR检测后，初步证明芪合酶基因已被整合到烟草基因组中，并可以正常转录。

RT-PCR and Real-time PCR analysis revealed that mRNA levels of the FK506-binding protein 12 (FKBP12) and two unknown genes were higher in the diapausing pupae, whereas the ecdysteroid-regulated 16kDa protein (esr16), NADH dehydrogenase 1 alpha subcomplex subunit 6, and two unknown genes were higher in the pupae of diapause termination.

通过RT-PCR和Real-time PCR的方法，鉴定了滞育解除过程中有3个基因表达量在下调，有4个基因表达量在上调。这7个差异表达基因中有3个和已知的基因有较高的同源性：FKBP12，esr16和NADH dehydrogenase 1 alpha subcomplex subunit 6。

Methods The expression change of NMDAR1 in cerebral subcortex after CPR was assessed by half-quantity RT-PCR.

应用半定量RT-PCR方法观察亚低温对心肺复苏大鼠大脑额叶皮层NMDAR1表达的改变。

Methods After transfection,a stable MRP-overexpressed subline named EJ/MRP was established.Gene expression of MRP and mdr1 were detected by using RT-PCR and immunohistochemistry methods.

将全长MRP cDNA转染膀胱癌EJ细胞，建立稳定表达MRP的亚克隆EJ/MRP，用RT-PCR、免疫组织化学方法检测了MRP和mdr1基因的表达，并检测了其对11种化疗药物的敏感性。

Methods］ MTT assay was employed to test the lethal concentration 50 (IC50) of sensitive germline (SK-OV-3) and ovarian cancer cell subline induced by ADR (SK-OV-3/adr) treated by 4 drugs, resistance index and reverse multiple were calculated. The changes of drug content were observed by fluorescence microscope, the intracellular accumulation of ADR was evaluated by fluorospectrophotometry, the changes of mdr 1/P-gp resistance were observed respectively by RT-PCR and Western blot.

方法］MTT法检测药物对敏感细胞株（SK-OV-3）和诱导的耐药细胞株（SK-OV-3/adr）的半数致死浓度（IC50），计算耐药指数和加逆转剂后的逆转倍数，荧光显微镜观察不同时间细胞内的药物含量变化，荧光分光光度法测定细胞内ADR的含量，RT-PCR和Western blot法检测细胞耐药前后mdr1/P-gp的变化。

Moreover, RT-PCR results revealed that the expression of AtSPX3 in phrl was reduced, suggesting PHR1 may function at the upstream of AtSPX3. Transgenic lines with RNAi of AtSPX3 showed supersensitive to Pi-starvation compared with Col.

另外，缺磷条件下AtSPX3基因启动子驱动的GUS活性在phr1突变体中受到抑制，定量RT-PCR的结果也显示，缺磷条件下该基因在phr1突变体中的表达受到抑制，说明在磷信号的传递途径中，PHR1在AtSPX3的上游。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。