查询词典 RT

Making use of RT-PCR to examine the expression of c-fos and c-myc semiquantitatively which is controlling gene of VSMC.

采用 RT-PCR 法半定量检测 VSMC 的调控基因c-fos 和 c-myc 表达的改变规律。

According to the published genomic sequence of TMV ,five sets of PCR primers were designed and five nucleotide segments were amplified by RT-PCR from the total RNA of the TMV infected Rehmannia glutinosa leaves.

根据已发表的TMV基因组的核苷酸序列设计5对简并引物，以感染TMV的地黄叶片总RNA为模板，经RT-PCR扩增获得TMV-RH的5个核苷酸片段的cDNA。

If you will be running RT 3.0.x or earlier under FastCGI, you need support for setgid perl scripts.

如果您要在 FastCGI 下运行 RT 3.0.x 或者更早，您需要 setgid 的 perl 脚本的支持。

A single faecal RT-PCR result on its own is meaningless: if the cat is intermittently shedding FCoV, by the day following sampling the cat may have changed from being a shedder to a non-shedder or vice versa.

单一次的粪便RT-PCR结果是毫无意义的：如果猫咪是间歇性的排出FCoV，从猫咪身上取样的隔天也许会从会散播变成为不会散播，反之亦然。

This study was designed to clone the duck FABP gene based on the sequences of chicken and mammal FABP gene by comparative genome and bioinformatics approaches; to investigate the expression characterization of FABP genes by RT-PCR; to detect the polymorphisms of FABP genes by DNA sequencing, PCR-SSCP methods, and to investigate the effects of FABP genes polymorphisms on growth and body composition traits in Kunshan sheldrake, Cherry Valley Meat duck and so on.

本研究以鸡、哺乳动物FABP基因序列为基础，通过比较基因组学和生物信息学等方法克隆鸭的FABP基因；采用RT-PCR方法研究FABP基因的组织表达特性。

Inaddition,we extract total RNA from cultural U251 cell of humanglioma,make coding gene extron amplification of TfR through ReverseTranscription-Polymerase Chain Reaction,construct expressionplasmid of pCDNA3.1-TfR after digestion of enzyme andconjunction,do transfection to U251 cell by liposome afteridentification,cause excess expression,detect the efficiency of transientexpression of TfR protein by pEGFPN1-TfR, sieve positive cell ofpCDNA3.1-TfR through G418, choose positive cell clone, detect theexpression of TfR in anteroposterior period through Western blot,FCM,cell immunochemistry and mRNA through RT-PCR, identify the effect of transfection.

另外，培养人脑胶质瘤U251细胞，进行细胞总RNA抽提，运用RT-PCR方法进行TfR基因的完整编码区外显子的扩增，经过酶切、连接后构建pCDNA3.1-TfR的表达质粒，鉴定正确后按脂质体转染方法转染人胶质瘤U251细胞，使其过量表达，通过pEGFPN1-TfR检测其瞬时转染的效率；用G418对稳定表达pCDNA3.1-TfR的细胞进行筛选，挑选阳性细胞克隆，运用Western blot、流式细胞检测技术和细胞免疫化学以及mRNA半定量的方法检测TfR在转染前后的表达水平，鉴定转染的效果。

RT-PCR discovered that BcMF14p was expressed prominently in flower buds, flower and silique, but no expression in vegetative organs, this indicated that BcMF14p may be involved in the signal transduction during reproduction development of B. campestris var. purpurea.

对该信号分子的表达进行RT-PCR分析，结果显示BcMF14p只在紫菜薹花蕾、花、角果等生殖器管中表达，在茎、叶等营养器官中不表达，说明BcMF14p可能参与紫菜薹生殖发育过程中的信号传导。

RT-PCR results indicate that gene is expressed only in the silk gland in all the tissues tested.

RT-PCR表明该基因仅在本文所检测的家蚕丝腺中表达。

RT-PCR detection and analysis of gene chip data showed that silkworm mago nashi gene expressed in all the stages detected during silkworm development, including embryo, larva, pupa and moth. However, higher expression could be visualized in gonads and silk gland compaired with other tissues.

RT—PCR检测和组织芯片数据分析结果表明，家蚕mago nashi基因在胚胎、幼虫、蛹和蛾期都有表达，在5龄第3天幼虫的各组织中也均有表达，但在生殖腺和丝腺中的表达量高于其它组织。

Using RT-PCR analysis, RGS7 was expressed from fertilization to 96 hours post-fertilization of Zebrafish. Expression of RGS7 was found in brain、aorta ventralis、intermediate cell mass and somite by whole-mount in situ hybridization.

因此，利用RT-PCR以及全胚体原位杂合技术，结果发现在受精后0~96小时的斑马鱼胚胎中皆可侦测到RGS7的表现，其表现的位置是在斑马鱼胚胎的脑部、腹侧主动脉、中期细胞团及体节等组织部位。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。