查询词典 RT

In this study, semi-quantitative RT-PCR, ABC immunohistochemistry assay, flow cytometry analysis and luciferase/luciferin assay were used to study the expression and function of CD39 in seven hematopoietic cell lines.

采用半定量RT-PCR、ABC免疫酶标和流式细胞术以及荧光素/荧光素酶法，研究了七个血源细胞系中CD39的表达和功能。

In order to detect the expression of N5, N10-methylene-tetra hydrofolate reductase gene in Epstein-Barr virus transformed human B lymphoblast, as well as the cDNA sequence, human peripheral blood was transformed by EBV to build B lymphoblast cell line. Total RNA was isolated from the cell culture. Variant segments of MTHFR cDNA were amplified using RT－PCR protocol and analysed by polyacrylamide gel electrophoresis.

为检测用EB病毒转化建立的永生性人B淋巴母细胞株中N5，N10－亚甲基四氢叶酸还原酶基因的表达及其cDNA序列，用EB病毒转化人外周血B淋巴细胞，建立永生性细胞株后，从培养细胞中提取总RNA，用RT-PCR法扩增MTHFR基因cDNA的不同片段，进行PAGE电泳分析和cDNA序列测定。

Methods OS732 was treated with 10^(-5)MATRA for 6 days, then we observed cell morphology, counted cell number, analyzed cell cycles by flow cytometry and detected mRNA expressions of cyclinD (Dl, D2, D3), CDK4, P21 by semi-quantity RT-PCR. Treatmed with 10^(-5)MATRA, The nucleus of OS732 cells became small and condensed, and cytoplasms expanded.

用10^(-5)MATRA作用骨肉瘤细胞OS732， 6d后，进行细胞形态学观察、活细胞计数、流式细胞仪分析细胞周期、半定量RT-PCR检测细胞周期调控蛋白CyclinD(D1、D2、D3)、CDK4、P21 mRNA表达的改变。

Methods The level of EGF mRNA in the cortex and medulla of porcine kidney was measured by RT-PCR technique.

采用RT-PCR技术分别检测猪肾皮质和髓质中的EGF mRNA活性。

By immunohistochemistry and semiquantitative RT-PCR analysis, we were the first to demonstrate that AdM mRNA and immunoreactive AdM exists in the rat brain distributed in cerebral cortex, paraventricular tissues, hypothalamus, mesencephalon, medulla oblongata and cerebellum, and AdM mRNA was also found in the human brain.

应用免疫组织化学和半定量RT-PCR方法，首次发现在大鼠的脑内，包括大脑皮层、室旁组织、下丘脑、中脑、延髓和小脑均有AdM mRNA及AdM的免疫活性物质存在；在人脑内也含有AdM mRNA。

Methods: Twenty-four Kkay mice were divided into three groups(n=8):diabetes group,methionine-diet group,folic acid and vitamin B12 treatment group. C57Bl/6 mice were used as controls(C57). Four months later,the mice were sacrificed and their serum levels of Hcy,folic acid and vitamin B12 were measured by fluorescence polarization immunoassay. Pathological changes in kidneys were examined with the periodic acid schiff method with periodic acid-silver methenamine staining under electron microscope. Expression of MMP-9 protein or mRNA was detected by RT-PCR with immunohistochemical staining. Results: Different morphological changes of DN were found in Kkay mice.

Kkay糖尿病鼠随机分3组：糖尿病组、高蛋氨酸组和叶酸、维生素B12组，C57BL/6鼠为对照(C57)，以相应饲料喂养4个月后取材，测定血糖、血同型半胱氨酸、叶酸和维生素B12，以PAS、六氨银染色法光镜下及超微电镜观察各组DN病变，比较各组肾小球硬化指数；以免疫组化和半定量图像分析肾小球MMP-9蛋白表达，并以RT-PCR检测肾脏MMP-9 mRNA水平。

The method on trans fatty acids analysis in food is very important. The effects of methylate sodium, potassium hydroxide, sulfuric acid and boron trifluoride in methanol and their different concentration and dosage on TFA analysis were studied. The chromatographic condition and the influence of time and temperature on the esterifation of TFA using boron trifluoride in methanol were optimized. A method for analyzing TFA were established by GC based on boron trifluoride in methanol and RT-2560(100m×0.25mm×0.2μm) capillary column.

研究了甲醇钠－甲醇、氢氧化钾－甲醇、浓硫酸－甲醇和三氟化硼－甲醇4种甲酯化试剂的浓度、用量及甲酯化时间和温度对反式脂肪酸检测的影响，优化了色谱条件，建立了以三氟化硼－甲醇法甲酯化，RT-2560(100m×0.25mm×0.2μm)毛细管柱为基础的反式脂肪酸气相色谱检测方法，C18：1-9 t， C18：1-11 t， C18：2-9 t， 12 t和C22：1-13 t 4种反式脂肪酸的最小检出浓度在0.774.08 mg/kg之间。4种反式脂肪酸不同添标浓度回收率为98.44%101.23%，重复测定的相对标准差在0.22%1.31%之间，有较好的准确度和重现性。

RT-PCR was applied to detect changes of hepatic hepcidin gene expression on model different stage. Bone density was detected and decalcification bone microtome section and hepatic histological section were observed and analyzed on the 14th day. Results Compared with control group, hepatic hepcidin gene expression of test group on different stage had obvious differences.

d每天灌胃，14d后形成骨质疏松模型；在实验组中采用RT-PCR方法分别观察灌胃第1、3、5、8、9、10、12、14d肝脏铁调素基因表达变化；要第14d还检测大鼠骨密度、分别脱钙骨切片、观察肝组织切片。

The results showed that the virus isolation and identification could generate considerably reliable results but the procedure was complicated and costed long time; microtomy fluorescent antibody detection was fast, but the specificity was low and needed to exclude the false positive from the results. Sandwich ELISA detection combined the merits of the former two methods, but it generated the false negative results when the samples were in bad preservation. Compared with the others, RT-PCR was fast, high sensitive and especially useful for detecting the CSFV directly from the samples. The specificity could be raised by using the nested-PCR to detect the CSFV from the simples, especially when the simples were in bad preservation.

结果显示，病毒分离鉴定法准确性较高，诊断比较容易，但存在试验步骤繁琐，所需时间长的缺点；荧光抗体检测法所需时间较短，但需要经验比较丰富人员来判定结果，且存在假阳性结果，特异性比较差；RT-PCR检测法具有快速、敏感等优点，特别是样品保存不理想时更有价值，利用套式PCR可以提高检测的特异性；夹心ELISA检测法具有快速、敏感等优点，但在样品保存不理想时会出现假阴性结果。

Methods RT-PCR and RACE methods were applied to clone the full-length panallergen gene from Caryota mitis pollen.

方法利用RT-PCR结合RACE技术克隆短穗鱼尾葵花粉中泛变应原profilin的全长基因，并进行序列分析。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。