查询词典 RT

Two 15nt complementary ssDNA primers were ligated into double stranded satRNA.Complete sequence of satRNA was obtained by RT-PCR using these 15nt ssDNA as amplification primers.Phylogenetic analysis showed that the identity of this 383nt satellite and some documented satRNAs was 72.6 to 99.5% at the nucleotide level.

以常规引物对感病植物的总RNA进行RT-PCR扩增，获得CMV RNA3全长克隆，经测序显示该RNA3序列属于CMV亚组Ⅱ；通过在卫星dsRNA两端分别加上15nt的单链DNA接头，以接头互补序列为引物进行扩增获得一个383nt的卫星RNA的全长序列。

Finally a all-digital recorder system based on RAID5 which has a RT-Linux real-time software operation system and intermediate/video frequency acquisition card driver to overcome such problems as real-time quality reliability chunnel expansibility and standardization.

最终提出了以RT-Linux实时操作系统为软件平台和专门设计的中频和视频采集卡驱动为底层硬件平台的基于RAID5阵列的全数字记录系统，全面解决了记录实时性、可靠性以及通道扩展性和标准化的技术难题。

Based on the theory of large eddy simulation and the method of VOF , the initial breakup process of fuel liquid jet, which eject from the nozzle exit at the time scale of micro-second level, is investigated. The mechanism of primary atomization is analyzed, and the initial breakup model of liquid jet is presented. By modeling and numerical simulation of atomization for a diesel nozzle under the given conditions, the initial umbrella-like spray shapes are obtained, which fits better with the experimental results. Meanwhile, the droplets secondary breakup process, such as leeward RT breakup, dumbbell-like breakup and droplets coalescence, etc. are exhibited visually.

基于大涡模拟LES理论和VOF方法，考察了燃油喷雾初始时刻即时间尺度为微秒级的液柱破碎过程，分析了初始破碎的机理，给出了该时间尺度下液柱初始破碎过程的模型；通过对一特定条件下的柴油机喷油器的建模和喷雾过程的大涡数值模拟，获得了液柱初始时刻"伞状头部"的喷雾形态，所得计算结果与相应的试验数据符合较好；数值模拟还直观地展现了液滴背风RT破碎、哑铃型破碎以及液滴的聚集融合等液滴的二次雾化过程。

Methods The designed flow chamber had provided flow shear stress at 0.5, 1 Pa (5 and 10 dyn/cm^2) for a period of 1 h, 3 hand 6 h respectively. Semi-quantitive RT-PCR was used to detect the expression of megalin/cubilin mRNA in cultured renal tubular epithelial cells.

应用流室系统提供0.5、1Pa(5、10 dyn/平方公分)的剪切应力处理培养的肾小管上皮细胞，分别作用1、3、6h后，用RT-PCR法检测megalin/cubilin mRNA的表达。

The protein and mRNA level of MCP-1 and IL-8 in U937 cells were determined by RT-PCR and ELISA.

用ELISA和RT-PCR方法测定MCP-1和IL-8的蛋白和mRNA水平。

Results: A clear-cut endocardium image was obtained by both RT-3DE and CMRI in all but 1 volunteer.

RT-3DE是一种定量评价左心室容量-时间曲线的可行、准确的新方法。

It was proved by RT-PCR that Pinb gene expressed itself in grain endosperm.

RT-PCR证实了Pinb基因在籽粒胚乳中的表达。

Expressions of eNOS and COX2 mRNA and proteins were assessed by immunohistochemical technique and RT-PCR.

用免疫组化和RT-PCR技术检测肺组织eNOS和COX2 mRNA和蛋白表达。

Methods The cDNA coding sequencing of enterokinase light chain was amplified by RT-PCR from mouse dodecadactylon mucosa and cloned into pET32a expression plasmid. The recombinant enterokinase light chain was expressed in BL21(DE3) and purified with Ni-affinity chromatography.

采用RT-PCR从C57BL/6J小鼠的十二指肠肠系膜黏膜组织中钓取肠激酶轻链的cDNA，将其克隆入pET32a原核表达载体中，并在大肠杆菌BL21(DE3)中进行表达，然后以镍亲和层析法对表达产物进行纯化。

The objective of the study was to obtain the gene of bovine enterokinase light chain,which would be used in the cleavage and purification of fusion proteins.The fragment of bovine enterokinase light chain cDNA was obtained by RT-PCR from a sold bovine′s duodenal mucosa, then cloned into the pUCmT cloning vector and sequenced.Compared with the sequence deposited in GenBank,the cloned gene sequence is correct.

为克隆表达牛肠激酶轻链编码基因，以期应用于融合蛋白的切割与纯化，从市售北方黄牛十二指肠组织中提取总RNA，以RT-PCR方法扩增其cDNA片段，将此片段克隆于pUCmT载体中，经过特异性限制性内切酶酶切分析片段正确后，进行全序列分析。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。