查询词典 RT

For cloning antifreeze protein gene from a desert darkling beetle Microdera punctipenis dzunarica in Xinjiang, the primers were designed according to the core sequence of AFP gene deposited in GenBank, and the cDNA fragment about 294 bp named as MpAFP5 was amplified with the RT-PCR and 3'-RACE technique. Sequence analysis revealed that the cloned cDNA fragment named as MpAFP5 coded the mature peptide of AFP.

根据GenBank中已发表的昆虫抗冻蛋白基因的保守序列设计引物，利用RT-PCR技术结合3'-RACE扩增的方法，从新疆荒漠昆虫准噶尔小胸鳖甲Microdera punctipenis dzunarica中获得了长约294 bp不含信号肽的抗冻蛋白cDNA片段，命名为MpAFP5，其全长序列为363 bp(GenBank注册号为：AY821792)。

Methods: RT - PCR and immunoblot were used to analyse DCC gene expression on 36 cases of large intestinal Cancer.

采用RT-PCR和免疫印迹法检测分析了36例大肠癌抑癌基因DCC在mRNA和蛋白水平的表达。

METHODS:To detect the expressions of CD44v mRNA and DCC mRNA in PLC by RT-PCR.

应用RT-PCR检测30例原发性肝癌中CD44v mRNA和DCC mRNA的表达情况，结合其转移相关的临床病理指标分析它们间的关系以及在转移中的作用。

Methods: Expression levels of MMP10 gene and protein in human spontaneous abortion decidua were determined by RT-PCR, Western blotting and distribution by immunohistochemistry.

利用RT-PCR和Western blotting技术分别检测MMP10基因和蛋白在人正常蜕膜和自然流产蜕膜的表达水平、利用免疫组织化学技术检测MMP10在人正常蜕膜和自然流产蜕膜组织中的分布。

The liver fibrotic model was induced by CCl4 i.p for 5wk. Telmisartan was given at the same time model establishment in each intervened group by administration lasted for 6wk. Decollate to get the blood of all rats after 7 weeks . ALT、AST and ALB of serum was detected by automatic biochemistry equipment. Hyaluronic acid was detected by ELISA detection. Liver and spleen was obtained to calculate the index. The liver pathological changes were observed in each group of rats after HE staining to evaluate the therapeutic effect of Telmisartan; TGF-β1、PPAR-γand PDGF of liver tissue was detected by the method of immunohistochemistry respectively; The mRNA expression of TGF-β1、MMP-1、TIMP-1、α-SMA、PPAR-γand iNOS was detected by RT-PCR detection, respectively.

腹腔注射CCl4制备SD大鼠肝纤维化动物模型，连续灌胃6周，处死动物取血，检测血清ALT、AST、ALB；ELISA法测定血清透明质酸；取肝、睥，并计算肝、脾指数，组织切片HE染色后进行组织病理学疗效评估；用SP免疫组化法测定肝组织TGF-β1、PPAR-γ及PDGF蛋白表达；半定量RT-PCR方法检测肝组织TGF-β1、MMP-1、TIMP-1、α-SMA、PPAR-γ和iNOSmRNA的表达。

Hepcidin mRNA was expressed in the mice brain.Total RNA was extracted from choroid plexus, cerebral cortex, diencephalon and mesencephalon, cerebellum, medulla oblongata and pons in mice. RT-PCR analysis demonstrated that hepcidin mRNA was expressed in all areas of mouse brain, in choroid plexus the mRNA expression was highest, although its level is generally lower than that of liver. The PCR products were purified and verified the sequence.

小鼠脑内有hepcidin基因表达提取小鼠脑脉络丛、大脑皮层、间脑及中脑、小脑和脑桥与延髓组织总RNA进行RT-PCR表明，大脑皮层、间脑和中脑、小脑、脑桥与延髓、脉络丛均有hepcidin表达，其中以脉络丛表达较高，但与肝脏相比，表达量较低。

The best optimum conditions of the Agrobacterium mediated genetic transfornation were that leaves explants were immersed in the diluter 10 times Agrobacterium bacilli at OD600 of 0.6 for 8 minutes after being pre-cultured for 2 days,and then co-cultivated for 3 days.Then these explants were transferred to selected and rooting medium which contained 50 mg/L Kanamycin and 500 mg/L Carbenicillin.According to the transformation system, 54 transgenic plants were initially screened,then after PCR analysis and RT-PCR analysis 25 transgenic plants were obtained.

根癌农杆菌介导的芪合酶基因遗传转化的最佳条件为：将预培养2 d的烟草外植体，用稀释10倍的GV3101菌株菌液（OD600=0.6）浸染8 min后，共培养3 d，然后转入含卡那霉素50 mg/L和羧苄青霉素500 mg/L的筛选分化培养基上诱导分化，待抗性芽长到2～3 cm后，转入含Km 50 mg/L和Cb 500 mg/L的筛选生根培养基上进行筛选，在以上最佳遗传转化条件下，初步筛选到54株转化体，经PCR和RT-PCR检测获得25株转化烟草植株。

It was a new mousegene in GenBank and named as SRG-L(spermatogenesis related gene expressed in the latestages of spermatogenic cells). The sequence of this cDNA has been deposited in GenBankwith the Accession No. AY352586. 2. Analyses of expression characteristics of SRG-L during mouse spermatogenesis.The results of RT-PCR and Northern Blotting indicated that no expression signal of thisgene could be observed in primitive type A spermatogonia and type B spermatogonia. Asignal first appeared in diplotene/pachytene spermatocytes, and was significantlyintensified in round spermatids and elongating spermatids, findly went down to beundetectable in mature spermatozoa.

RT-PCR和Northern Blotting分析表明：该基因在早期A型和B型精原细胞中不表达，从双线期初级精母细胞阶段开始表达并逐渐上调，在粗线期初级精母细胞、圆形精子细胞及长形精子细胞中均检测到高水平的mRNA，但在成熟精子中消失，具有明显的阶段特异性表达特征，并且组织特异性表达分析显示该基因在睾丸组织中的呈高表达。

According to the gallus colony stimulating factor(colony-stimulating factor,CSF)cDNA sequences published by GenBank,a pairs of specific primer was designed,the local dorking was injected 100 μg/mL ConA,total RNA was extracted from feeding 24 hours lymphocyte from spleen,then dorking colony stimulating factorcDNA fragment was cloned by RT-PCR technology.

根据GenBank中报道的红原锦鸡集落刺激因子(Colony-stimulatingfactor，CSF)cDNA序列，利用Primer Premier5.0软件设计一对特异性引物，对本地肉鸡注射100μg/mL ConA试剂，饲养24 h后，取脾脏提取淋巴细胞总RNA，利用RT-PCR技术克隆集落刺激因子的cDNA片段。

According to the gallus colony stimulating factor (colony-stimulating factor, CSF) cDNA sequences published by GenBank, a pairs of specific primer was designed, the local dorking was injected 100 μg/mL ConA, total RNA was extracted from feeding 24 hours lymphocyte from spleen, then dorking colony stimulating factor cDNA fragment was cloned by RT-PCR technology.

根据GenBank中报道的红原锦鸡集落刺激因子(Colony-stimulatingfactor， CSF)cDNA序列，利用Primer Premier5.0软件设计一对特异性引物，对本地肉鸡注射100μg/mL ConA试剂，饲养24 h后，取脾脏提取淋巴细胞总RNA，利用RT-PCR技术克隆集落刺激因子的cDNA片段。

Finally, according to market conditions and market products this article paper analyzes the trends in the development of camera technology, and designs a color night vision camera.

最后根据市场情况和市面上产品的情况分析了摄像机技术的发展趋势，并设计了一款彩色夜视摄像机。

Only person height weeds and the fierce looks stone idles were there.

只有半人深的荒草和龇牙咧嘴的神像。