查询词典 RT.

Students attend a boarding school would cultivate their independence as apa rt from their parents.

离开父母上寄宿学校的学生将会培养他们的独立性。

Methods Turning tests induced by apomorphine and forelimb functional tests were performed. Also, the expression of TH mRNA in mesencephalon of the rats was detected by semi-quantitative analysis of one step RT-PCR.

利用阿朴吗啡诱发旋转试验和前肢功能试验，检测PD大鼠的神经行为学特点，一管法逆转录聚合酶链反应半定量检测其中脑TH mRNA的表达。

A pair of primers was designed according to the coat protein gene of Arabis mosaic viru and used to amplify a 990 bp partial gene of the cp by reverse transcription-polymerase chain reaction, the 990 bp partial gene was cloned into the pEGX-9p-1, a prokaryotic expression vector fused with GST. The recombinant vector was transformed into E. coli BL21. The fusion protein was successfully expressed in E. coli BL21 induced with the IPTG at 37℃.

依据报道的ArMV外壳蛋白基因(coat Protein， Cp)序列设计引物，通过RT-PCR 扩增得到长约990bp的ArMV cp基因亲水基团部分片段，将目的片断克隆到原核表达载体pEGX-6p-1中，构建ArMV cp基因与GST蛋白融合表达载体pEGX-cp，重组载体化大肠杆菌BL21，经IPITG诱导后，融合蛋白GST-cp得到了特异表达。

Results The well-developed arborization and interconnection of podocytes were found in control group.

用RT-PCR、Western印迹和间接免疫荧光染色检测各时间点podocin mRNA和蛋白的表达及分布。

Compared with L7 cells not induced by Dox, 7 genes were upregulated and 12 genes were downregulated at Dox 6μg/ml, of which 4 were selected for RT-PCR to confirm the result from cDNA array, expression of four genes were consistent with that from cDNA array.

选择含有265个转移相关基因的cDNA微阵列膜，以Dox不诱导为对照，检测在Dox6μg／ml诱导下，L7细胞中差异表达的转移相关基因。

Objective: To investigate, 1 whether the body fluid of Ascaris lumbricoides could induce apoptosis of human alveolar epithelial cells (A549 cells) in vitro, and if there would be a possible relationship of ABF concentration or inducing time with the extent of apoptosis; 2 if the expression of bcl-2/bax would involve in and how it changes during the inducement of the apoptosis of A549 cells.

目的：用人蛔虫体腔液(Ascaris body fluid，简称ABF)体外诱导人肺上皮细胞株(A549)，观察其是否能够诱导靶细胞凋亡及凋亡与ABF浓度和作用时间的相互关系；运用RT-PCR方法检测靶细胞诱导过程中bcl-2／bax基因的表达变化，探讨其凋亡与基因表达的相互关系。

The OsCMO contains 10 exons and 9 introns and encodes a protein with 410 amino acids,which shows 62﹪, 51﹪, 51﹪, 51﹪ and 50﹪ identities in amino acids to CMOs from Arabidopsis thaliana,Atriplex hortensis, Amaranthus tricolor, Suaeda liaotungensis and Beta vulgaris respectively. Semi-quantitative RT-PCR assay revealed OsCMO was significantly up-regulated in the rice seedlings under salt, cold and drought stresses. It suggests that OsCMO might play an important role in rice responses to abiotic stresses.

该基因含有10个外显子和9个内含子，其开放阅读框编码一条长为410个氨基酸残基的多肽，与拟南芥、山菠菜、苋、碱蓬和甜菜等植物的胆碱单加氧酶的氨基酸序列的一致性50﹪～62﹪。mRNA分析表明OsCMO基因在水稻幼苗组织中的表达受高盐、低温和干旱等胁迫的上调诱导表达，表明该基因可能在抵御非生物胁迫中发挥重要作用。

All patients underwent hearing tests including pure tone audiometry and impedance audiometry before and after RT.

所有病例放疗前后均经耳部检查，电测听及声阻抗测听，放疗后随访时间为6~24个月。

It is reported that SarA regulates the transcriptions of lipA encoding lipase, zinC encoding membrane-associated zinc metalloprotease and atlE encoding autolysin. SarA exhibiting an up-regulation on lipA and zinC, and down-regulation on atlE were demonstrated by reverse-transcription PCR and reporter gene lacZ analysis respectively.

RT-PCR和lacZ报告基因的分析结果显示，在表皮葡萄球菌ATCC35984中，SarA对atlE表达起负调控作用，而对lipA和zinC（膜相关锌金属蛋白酶基因）的表达则有正调控作用。

Methods The clpE-deficient strain was constructed by LFH-PCR and identified by PCR and sequencing. The impact of clpE mutant on the virulence of S. pneumoniae was evaluated in a mouse model. In addition, we also studied the effect of clpE mutant on adherence and invasion of host cells. Real time RT-PCR was used to measure the mRNA expression levels of autolysin A, pneumococcal surface adhesion A, pneumolysin, pneumococcal surface protein A and neuraminidase The clpE gene was replaced completely by erm cassette.

用长臂同源多聚酶链式反应方法失活clpE基因，用PCR、测序鉴定缺失菌株，通过动物实验观察clpE基因缺失株毒力改变情况，同时用细胞实验比较clpE基因缺失株和野生菌对宿主细胞的粘附和侵袭能力，最后用实时荧光定量PCR分析自溶素(major autolysin A，lytA)、表面黏附素A(pneumococcal surface adhesion A，psaA)、溶血素(pneumolysin，ply)、肺炎球菌表面蛋白A（pneumococcal surface protein A， pspA和神经氨酸酶（neuraminidase， nanA）的表达。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。