查询词典 RT.

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

The replicons were detected and characterized by RT-FUR. IFA. Renilla Luciferase assay system and real time RT-FUR. respectively. Results It was shown that SLB2 mutant did out significantly affect the translation of the input RNA.

将突变体（包括相关的阳性和阴性对照复制子）分别线性化并体外转录成RNA后，取等量各转录体RNA，以脂质体法分别转染BHK-21细胞。

A m a n's he a rt is like a bird locked inside the c a ge of the body. When you d a nce, the he a rt sings like a bird a spiring to a fusion with God.

人的心就像是一只被关在肉体牢笼中的小鸟，当你跳舞的时候，心就会歌唱起来，想一只渴望与神结合和小鸟。

The unspliced and spliced forms of XBP1 stably expressing NS4B in HeLa cells, the transcriptional levels of ATF6, Grp78 and caspase-12, and the luciferase activity in XBP1 and Grp78 promoter reporter assays in HeLa and Huh-7 cells expressing NS4B were detected.

用 RT-PCR 和免疫印迹的方法检测稳定表达 NS4B 的 HeLa 细胞中的 XBP1；通过 RT-PCR 的方法在表达 NS4B 的 HeLa 和 Huh-7 细胞中检测 ATF6，Grp78 和 caspase-12 的转录，并且通过报告基因的方法分析 XBP1 和 Grp78 启动子活性。

Methods: Human luteinized granulosa cells were collected from patients undergoing in-vitro fertilization-embryo transfer.

收集体外受精－胚胎移植过程中的人黄素化颗粒细胞进行体外培养；应用RT-PCR法和免疫细胞化学法分别检测HB-EGF的mRNA和蛋白表达；应用实时RT-PCR法检测HB-EGF mRNA量的变化。

Objective To study the effect of Borna disease virus infection on the transcription of monoaminergic receptor genes in the brain tissues of neonatally inoculated rats.

目的分析博尔纳病病毒感染对新生大鼠脑内单胺类受体基因转录的影响。方法选择病毒滴度为2·0×106FFU/ml的BDV病毒液对新生大鼠进行颅内接种，接种量为10μl/只新生大鼠。30d后用RT-PCR和间接免疫荧光方法确定BDV感染情况；并采用半定量RT-PCR方法检测BDV感染大鼠脑组织中多巴胺2(D2)受体和5-羟色胺2α(5-HT2α)受体基因的mRNA转录情况。

Two cases of specimens collected in operation were studied by in situ RT????PCR, of which 50 cases were hemangioma: 26 cases proliferating hemangioma and 24 cases involuting hemangioma, and 12 cases were venous vascular malformation. The expression of Ang2 mRNA and Tie2 mRNA in hemangiomas and venous vascular malformation were detected by in situ RT????

PCR方法，对临床收集的62例手术切除的先天性皮肤血管标本（其中增生期血管瘤26例，消退期血管瘤24例，静脉型血管畸形12例）进行Ang2 mRNA， Tie2 mRNA表达水平的检测。

Total RNA was isolated from phloem of young shoots and retro-transcripted to cDNA by RT-PCR with specific primers, and the amplified cDNA fragments were sequenced for identification of subtypes. An effective RT-PCR technique was established for detecting citrus viroid types, and three types were recognized, i.e.

以田间柑橘枝条韧皮部为试材，提取总RNA，反转录成cDNA，再用不同类型类病毒的特异引物进行PCR扩增，鉴定出各个样品所携带的柑橘类病毒类型；通过测序分析各类型的序列，并与国内外报道的序列进行同源性分析，确定所测样品的亚型。

After PCR primer for FMDV and multiple PCR primers for type Asia I virus and differention of Cathay and PanAsia topotypes of FMD type O viruses were designed,the optimal conditions of RT-PCR and multiple RT-PCR were determined by comparing the products of PCR.

在大量比较国内外口蹄疫病毒流行毒株序列的基础上自行设计了检测FMDV通用型引物及FMDVAsiaI型、O型口蹄疫病毒不同基因型即：中国型与泛亚株的多重PCR引物。

PSMA7 shRNA transfection resulted in significant inhibition of CD44 expression without obvious effect on the expression of MMPs and TIMPs.

RT-PCR检测表明PSMA7 shRNA转染不影响MMP和TIMP的表达，RT-PCR和蛋白免疫印迹检测表明PSMA7 shRNA转染抑制CD44的表达。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。