查询词典 RT.

Gena has successfully developed a quick molecular diagnostic system through which may simultaneously identify and differentiate kinds of different subtypes of influenza A viruses including H5N1 which highly makes illness.

我们开发出了一个可同时检测流感病毒多种亚型，包括高致病的 H5N1型的快速分子诊断系统，在一个管内采用多重逆转录 RT-PCR 法扩增可能潜在的流感病毒基因组的多个特异靶点， RT-PCR 产物可用 xMAP 技术检测和鉴别。

The changes of the fruit cells during ripening and softening were studied used the scanning electron microscopy . A pectate lyase and a β-galactosidase gene were also cloned from mature banana fruit by RT-PCR and the expression of them were investigated by the semiquantitative RT-PCR.

主要结果如下： 1 通过对香蕉果实成熟软化过程中相关酶类，特别是近年报道的与果实成熟软化相关的13种酶活性的变化进行了研究，证明这些酶的活性变化多与香蕉果实成熟软化相关。

We also detected the serum of 8 patients 3 weeks after the treatment, PERV was negative in 5 patients, remained positive in 3 of the 8 patients.

术后3周收集8例病人血清，进行PCR与RT-PCR分析，发现有5例病人血清PCR与RT-PCR均阴转，3例仍为阳性。

The method was successfully used to quantitate the virus load in tiss...

最后用建立的方法对108份临床样品进行检测并同时进行病毒分离，荧光定量PCR方法检测出73份阳性样品且与病毒分离的符合率为100%，而常规RT-PCR只检测出54份阳性样品，表明本荧光定量RT-PCR法在检测猪瘟病料上具有潜在的应用价值。

The depend variables are displacement error, first saccade latency, manual RT, and eye-hand RT.

实验中记录定位距离误差、首次眼跳潜伏期、手动反应时和眼—手反应时差等指标。

Cell proliferation and viability were assayed 48h after transfection, and MDA-7 demonstrated selective inhibition of tumor cell growth, inhibitory rates of A549, Hela and HepG2 cell lines were 25%, 20% and 19%, respectively, but had no significant effect on human fetal kidney derived 293 cell line. Hela cell was screened by G418 for 2 weeks after pcDNA3-MDA-7 and monolayer colony was counted, its monolayer colony formation was 30% of cells transfected with pcDNA3. 0 vector. CMV-driven MDA-7 adenovirus vector was constructed. 293 showed no significant apoptosis during adenovirus packaging and the unpurified adenovirus titer was about 1×10〓pfu/ml. Cos 7, A549, Hela, HepG2 and Hep3B cell was infected with Ad-GFP at different MOI.

二。黑色素细胞分化相关蛋白-7（MDA-7）的克隆及功能研究：利用RT-PCR方法从5月龄人胚胎脾细胞扩增MDA-7的编码序列，经测序鉴定序列与文献报道一致后，与真核表达载体pcDNA3.0连接，构建pcDNA3-MDA-7表达载体，瞬时转染293、A549、Hela和HepG2细胞后抽提细胞总RNA，RT-PCR结果显示表达载体可介导MDA-7在不同细胞系中有效表达；转染48h后测定细胞增殖和活力，MDA-7可选择性抑制肿瘤细胞的增殖，对A549、Hela和HepG2细胞的抑制率分别为25％、20％和19％，但是对人胚肾来源的293细胞生长无明显影响。pcDNA3-MDA-7载体转染Hela细胞后，以G418筛选2周后计数单层细胞集落形成数，计数仅为转染pcDNA3.0空载体的细胞的30％左右。

By using new methods of isolating and purifying RNA of NDV, we improved the methods for detecting NDV based on Reverse transcriptase polymerase chain reaction and managed to detect velogenic NDV directly in 17 cloacal swabs specimens from different flocks including the 15 ones.

结果表明，生物学试验MDT和ICPI与ELISA阳性结果的强毒感染符合率为93.3％（14/15），而RT-PCR结果与ELISA阳性结果的强毒感染符合率为94.1％（16/17），而生物学试验加上RT-PCR结果与ELISA阳性结果的强毒感染总符合率为93.8％（30/32）。

Drug sensitive test and three-dimensional test220 strains of Pa were isolated from hospitalized patients between 2003 and 2007. K-B method was used to tested the susceptibility of 10 different antibiotics. IRPa was screened by testing the minimal inhibitory concentration of imipemem by using agar diluiion method.The susceptibility of these IRPa to the antibiotics was analysised. Three-dimensional test was used to identify the different kinds of beta lactamases from 220 strains of Pa.2.Carbarpenems hydrolytic enzyme genes and oprD2 gene were detectedamong the selected IRPa strains, PCR method was performed to detect carbapenemase genes which included GES、KPC、SPM、VIM、IMP、GIM gene and the oprD2 gene;Multiplex PCR were used to detect OXA genes and plasmid-mediated AmpC beta lactamase genes; The expression of the chromosomal AmpC beta lactamases and oprD2 genes in IRPa strains were analyzed by Real-time PCR.3.Identification and characterization of integronsIntegrase gene was detected by PCR, and the classification of integrons was performed by using restriction fragment length polymorphism.PCR was performed to detect the qacE△1-sull gene,and the gene cassetes which are located at variable region of integrons in the strains were detected to be positive.

方法1、药敏实验和三维实验收集2003～2007年临床分离的220株Pa，对这些菌株采用K-B法测定10种临床常用抗生素的药敏情况，同时采用琼脂稀释法检测亚胺培南的最低抑菌浓度(Minimal inhibitory concentration，MIC)，筛选出对亚胺培南耐药的铜绿假单胞菌，并分析其对其它抗生素的药物敏感率；采用三维实验的方法分析220株Pa产β内酰胺酶的类型。2、碳青霉烯类水解酶和oprD2蛋白的检测针对鉴定的IRPa菌株，采用普通PCR方法检测具有碳青霉烯水解作用的β内酰胺酶耐药基因(GES、KPC、SPM、VIM、IMP、GIM基因)和oprD2基因，采用多重PCR的方法检测OXA型基因和质粒携带的AmpC酶基因，用荧光定量RT-PCR方法检测oprD2蛋白基因表达情况；同时对产AmpC酶的Pa(25株，含IMP耐药和敏感株)用RT-PCR方法检测AmpC酶基因的表达量情况。3。

Dhar AK, Roux MM, Klimpel KR. Detection and quantification of infectious hypodermal and hematopoietic necrosis virus and white spot virus in shrimp using real-time quantitative PCR and SYBR Green chemistry［J］.

研究过程发现SYBR Green I实时荧光定量RT-PCR与巢式RT-PCR相比，具有线行关系好、线性范围宽等特点；并且，扩增和检测在同一管内完成，不需要开盖，不需要电泳检测PCR产物，有效解决了污染问题［10］。

MNF of RG increased significantly (P.01). Polyamines in urine before RT and after RT were 40.00±11.93 and 15.75±4.91 of RG and 38.26±11.50 and 21.51±6.24 of CG respectively. Polyamines decreased significantly after radiotherapy (P.01) especially in RG. The major toxic reactions were esophagitis, inspirative tract reaction and neutropenia.

尿多胺含量：研究组放疗前后分别为40.00±11.93和15.75±4.91；对照组为38.26±11.50和21.51±6.24，放疗后研究组值高于对照组，统计学有显著差异（P.01）。2组治疗的副反应主要是呼吸道、食管炎和骨髓抑制，研究组较重。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。