查询词典 RT.

The MCP gene was amplified from Red-spotted Grouper Nervous Necrosis viral genome by RT-PCR. The recombinant expression vector pRSET A-MCP was constructed and transformed into BL21 (DE3) plysS to express proteins with induction in different media, at different pH, or at different temperatures.

方法］采用RT-PCR技术扩增出赤点石斑鱼神经坏死病毒MCP基因，构建重组表达载体pRSETA-MCP，以其转化大肠杆菌BL21(DE3)plysS，在不同培养基、温度、pH值条件下进行诱导表达。

Methods vIL-10 gene was tranferred into rat glomerular mesangial cells by retroviral-mediated gene transfer techniques.The expression of vIL-10 was detected by RT-PCR,ELISA and its bioactivity was determined by mix lymphocyte reaction.

应用逆转录病毒载体介导的基因转移技术将外源性基因vIL-10转移到大鼠肾脏系膜细胞，应用RT-PCR、ELISA方法检测其表达，并通过对混合淋巴细胞反应的影响对其活性进行分析。

Low dose Yiweitang group; 4. Diethylstilbestrol group; 5. Model control group. After 4 weeks intervention, get the left ovarie, a part of hypothalami and pituitary gland from head-off rat, and observe expression of ER, FSHR and GnRH by immune histochemistry method . Then extract RNA from the rihgt ovarie and the rest hypothalami and pituitary gland, so as to measure the expression of GnRHmRNA 、 ERmRNA 、 FSHRmRNA and P 16mRNA by RT-PCR. And the cerebrum were made to homogenate to examine SOD, GSH-PX and MDA.

各组实验动物灌药4周后，断头处死，取部分下丘脑、垂体及左侧卵巢组织固定切片，免疫组化法检测 ER 和 FSHR 表达、 GnRH ；取剩余部分下丘脑、垂体及右侧卵巢组织提取总 RNA ，用于 RT-PCR 检测下丘脑 GnRHmRNA ，下丘脑、垂体的 P 16mRNA 及下丘脑、垂体、卵巢的 ERmRNA 、 FSHRmRNA 基因表达；另外取部分大脑组织制作脑组织匀浆，用于 SOD 和 GSH-PX 酶活力以及 MDA 含量的测定。

On the successful basis of bovine hepatic cell culture and competitive DNA template of pyruvate carboxylase gene,effects of propionate sodium and pyruvate sodium on mRNA of PC gene in cultured bovine hepatocyte in vitro was determinated quantitively by competitive RT-PCR.

在成功构建牛肝细胞培养模型和PC基因DNA竞争模板的基础上，采用竞争RT-PCR方法检测了丙酸钠和丙酮酸钠对体外培养新生牛单层肝细胞PC基因mRNA丰度的影响。

Propionate sodium ; pyruvate sodium ; pyruvate carboxylase gene ; mRNA level ; competitive RT-PCR ; bovine hepatocyte

丙酸钠；丙酮酸钠；丙酮酸羟化酶基因； mRNA水平；竞争RT-PCR ；牛肝细胞

Key words: propionate sodium; pyruvate sodium; pyruvate carboxylase gene; mRNA level; competitive RT-PCR; bovine hepatocyte

丙酸钠；丙酮酸钠；丙酮酸羟化酶基因；mRNA水平；竞争RT-PCR；牛肝细胞

There are some words and phrases you should understand in the context of RT before you begin.

在你开始之前，RT的上下文中有一些单词和短语你得理解。

Now that you are clear on the concepts, you're ready to start using RT.

现在，您已经清楚了概念，您准备好开始用 RT 。

In order to study the expression, subcellular distribution and the function of NS2 protein, the coding region of NS2 was amplified from the hindgut tissue of cockroaches infected with PfDNV by RT-PCR and then the recombinant prokaryotic expression vector pET28a-NS2 was constructed. The recombinant plasmid was transformed into E. coli BL21 (DE3) to express the 6×His fusion protein in the bacteria.

为了对该基因进行深入的功能研究，从感染了黑胸大蠊浓核病毒的蟑螂的后肠组织中通过RT-PCR得到ns2基因编码序列，将其构建于原核表达载体pET-28a，转化大肠杆菌BL21(DE3)获得融合表达产物。

Methods pVAX1-GRA8 was purified and transfected into Africa green monkey kindey vero cell lines,expression of GRA8 in vero cell lines were detected by RT-PCR and immunoblot with rabbits sera infected with Toxoplasma gondii.

大量提取纯化重组真核表达质粒pVAX1-GRA8，瞬时转染培养的vero E-6细胞，RT-PCR和免疫印迹法检测GRA8在细胞中的表达。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。