查询词典 RT.

Theexpression of M_1 and M_4 subtype receptor in the retina, choroids and posterior sclerawere detected with immunohistochemistry and the mRNA level of two receptors inthe three tissues were examined via RT-PCR.

实验结束后，通过RT—PCR对各组巩膜、脉络膜和视网膜内M_1和M_4亚型受体的mRNA水平进行检测，同时使用免疫荧光法检测两种受体在各个组织中的表达情况。

After 3-8 passages, the oral CAFs and the normal mucosa fibroblasts from tissue culture were observed under light microscope. Vimentin,α-SMA and Cytokeratin protein were detected by immunohistochemistry SP method on glass coverslips. VEGF, TGF-β1, MMP-9, Tenascin-C mRNA were analyzed by semi-quantitative RT-PCR.

用组织块培养法获得原代CAFs和正常粘膜成纤维细胞，0.25%胰蛋白酶消化传代和纯化细胞；通过形态学观察和免疫细胞化学染色对口腔CAFs和NMFs进行鉴定：采用半定量RT-PCR检测口腔CAFs和NMFs 4种基因的表达。

The coat protein gene of Cucumber green mottle mosaic virus was amplified by RT-PCR from total RNA of China import-based cucurbits seed and cloned into pMD18-T vector.

以直接从中国进口源性葫芦种子中提取的总RNA为模板，经RT-PCR扩增获得种子上携带的黄瓜绿斑驳花叶病毒(Cucumber green mottle mosaic virus， CGMMV)的外壳蛋白基因(LHP， Liaoning cucurbits seedprotein)，将其克隆到pMD18-T载体上进行序列测定。

Methods According to the examination result of vagina smears, 12 female rats were killed in proestrus, estrus, metaestrus and diestrus respectively, 3 for each, and their uterus and ovary were taken out, from which the total RNA and total protein were extracted for determination of transcription of SLC26A3 mRNA by RT-PCR, and expression of SLC26A3 protein by Western blot.

根据阴道涂片检测结果，将12只雌性Wister大鼠分别在动情前期、动情期、动情后期和动情间期断头处死，取出卵巢及子宫，提取总RNA及总蛋白，用RT-PCR及Western blot方法检测卵巢及子宫内SLC26A3在动情周期中各时期的表达。

Forty SD female rats were divided randomly into four groups : control (received normal saline, CTX, S1P+NS, S1P+CTX respectively. All rats were killed between the first and the second week in the diestrus stage after stopping medicines to compare the number of the primordial follicles,the primary follicles and the growth follicles. The expression of the Bcl-2 and Bax mRNA in the ovaries were examined by the RT-PCR.

40只雌性SD大鼠随机分为4组，即实验对照组、CTX组、 S1P+ NS组及S1P+CTX组，每组10只，于结束用药的第1～2周内的动情间期处死，观察卵泡数量的变化，并采用半定量RT-PCR检测卵巢组织Bcl-2及Bax 的mRNA变化。

The viruse was detected from dilutions of 10^6 by RT-PCR with specific primers.

灵敏性测定结果表明该特异性引物RT-PCR可从稀释10^6植物总RNA中检测出病毒。

The Erns gene,which is the first envelope protein,was amplified from the spleen of rabbits which were infected with classical swine fever virus by RT-nPCR method.

从兔脾组织中提取猪瘟病毒兔化弱毒株RNA，利用RT-PCR技术，扩增了猪瘟兔化弱毒的第一个囊膜糖蛋白基因Erns，并对其进行了序列分析。

In a word, we extracted first cotyledon of buckwheat total RNA, by method of RT-PCR and 3RACE, get the C terminal cDNA sequence of PI from Ukraine buckwheat, and do some bioinformatics analyse on this cDNA sequence of PI from buckwheat, such as gene characteristics, expression mode. All of this will be help for the forward research on functional protein and high performance of procaryon expression or eukaryon expression.

综上所述，我们利用RT-PCR和3′RACE技术首次从吉乌一号甜荞第一对子叶中克隆获得荞麦蛋白酶抑制剂基因并对其基因特性、表达模式进行生物信息学分析，同时构建了该基因序列羧基端原核表达载体，为今后进一步研究荞麦功能性蛋白的分子机制及高效原核、真核表达载体的构建奠定了基础。

Methods The immunofluorescency, Western blotting and RT-PCR were performed on mouse liver tissue obtained from 4 experimens pcDNA3.1(+-HBX and 1 normal control, 24 h after delivery the HBX gene eukaryon expression vector pcDNA3.1-HBX into mouse by the means of hydrodynamic injection.

实验动物分成模型组和对照组，模型组利用已构建的含有HBX基因的真核表达质粒pcDNA3.1-HBX，对照组以等量生理盐水代替，采用流体动力学法将质粒经尾静脉高压注入小鼠体内，24h后取小鼠肝组织，行免疫荧光、RT-PCR和Western blot法从不同水平检测HBX在小鼠肝组织内的表达情况。

The RT-PCR method can extract the intrahepatic bile duct cancer cell′s hTR gene.We construct the eukaryon express vector containing the antisense RNA and hammerhead ribozyme gene successfully.

RT-PCR法可调出其hTR基因有效片段；成功构建了针对hTR模板区的反义RNA基因和核酶基因的真核表达载体。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。