查询词典 RT.

The expression and clonality of TCR Vαand Vβsubfamilies within induced Tcells were analyzed by using RT-PCR and genescan technique.

收集诱导后第10天和第18天的细胞，RT-PCR-基因扫描分析其TCR Vα和TCR Vβ基因谱系变化特点。

Methods Recombinant adenovirus GDF-5 (Ad-GDF-5) containing green fluorescent protein and Ad-GFP were amplifiedand tittered. hBMSCs at passage 3 were infected with two viruses at different titers. At 2 days after intervention, GFP expression was observed using fluorescence microscope, and GDF-5 expression in hBMSCs was detected by RT-PCR.

将重组腺病毒GDF-5（adenovirus GDF-5，Ad-GDF-5）和空载腺病毒Ad-GFP 进行扩增并测定滴度，并以不同滴度两种病毒感染第3 代hBMSCs，干预后2 d 荧光显微镜下观察GFP 表达情况，RT-PCR 检测GDF-5 在hBMSCs中的表达。

The RT-nPCR can be used to differentiate pigs infected with wild-type CSFV from those vaccinated with the C-strain vaccine. It thus minimizes the possibility of miss-killed vaccinates during outbreaks.

本研究建立的RT-nPCR可以有效区分猪瘟强毒和弱毒，减少了未感染的免疫猪被误杀的可能性。

Mhods RT-PCR technique was used to assay the expression of MyoDI mRNA in peripheral venae blood from patients of gastrointestinal carcinoma and benign disease, 30 cases in each group.

采用RT-PCR方法检测30例胃肠恶性肿瘤恶液质和30例胃肠良性病变营养不良患者静脉血中MyoDImRNA表达。

RT-PCR was used to detect the mRNA expression of p16 and MGMT before and after treatment with 5-Aza-dC, respectively. Results: Promoter hypermethylation of the p16 and MGMT gene were detected in MGC-803 cells, and p16 and MGMT were absent before treatment.

应用5-Aza-dC处理体外培养的胃癌细胞MGC-803后，甲基化特异性PCR法检测用药前后细胞中p16和MGMT基因的甲基化状态，RT-PCR法检测用药前后细胞中p16和MGMT mRNA表达的变化。

Therefore it is interesting to clone genes related to lemma and palea. In this thesis, six genes from the lemma/palea related gene pool which is established by Liu (2003) were further studied. Those genes are salT gene (salt-induced protein), GA-SPY gene (gibberellin action negative regulator SPY-related protein), U2AF gene (U2 snRNP auxiliary factor, small subunit-related protein), kinesin-like gene, DnaJ-like gene, and EF hand gene. At first, the gene expression in leaves and 1~4 cm inflorescences of normal lemma/palea, smaller lemma/palea, and stunted lemma/palea was compared by Real Time RT-PCR.

从刘(2003)所建立水稻颖花发育相关候选基因库中，挑选6个基因：salT基因(salt-induced protein)、GA-SPY基因(gibberellin action negative regulator SPY-related protein)、U2AF基因(U2 snRNP auxiliary factor，small subunit-related protein)、kinesin-like基因、DnaJ-like基因、及EF hand基因，以水稻正常型颖花、小颖花突变体及内外颖退化突变体之叶片及1~4公分幼花序为材料，利用Real-Time RT-PCR分析，发现salT基因在突变体幼花序中大量表现。

At the same time, in situ RT-PCR of AFCVd for further detection proved the presence of AFCVd in apple leaf, mainly located in nucleus of mesophyll cell.

并利用原位RT-PCR技术做进一步检测，证明苹果叶片中有苹果皱果类病毒存在，主要分布在叶肉细胞的细胞核中。

Complementary DNA sequence of bastard halibut prion protein encoding gene was cloned and characterized through RT-PCR approach.

采用RT-PCR方法分离牙鲆蛋白质感染因子蛋白编码基因序列。

Methods In 488 cases of thyroid disease, 1% methylene blue 1.0-2.0mL was injected into the thyroid nodule or surrounding thyroid tissue 24 hours before operation. The dye-stained lymph nodes were detected at operation and RT-PCR method was used to determine MCUI in the clinically blue-stained lymph nodes.

对488例甲状腺疾病手术患者于术前24h用1%美兰1.0~2.0mL注射于甲状腺结节或周围腺体，术中显示蓝染淋巴结，采用RT-PCR法测定临床蓝染淋巴结中的MUC1。

At the same time,the mRNA levels of syk,Jak and Tnfsf13b were lower than the control by RT-PCR assay,but those of IFN-γ,IL-2 and IL-10 were higher.

RT-PCR结果提示：试验组syk，Jak和Tnfsf13b的mRNA表达水平降低，IFN-γ、IL-2和IL-10的mRNA表达水平升高。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。